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Salt-dependent compaction of di- and trinucleosomes studied by small-angle neutron scattering.
Hammermann M
,
Tóth K
,
Rodemer C
,
Waldeck W
,
May RP
,
Langowski J
.
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Using small-angle neutron scattering (SANS), we have measured the salt-dependent static structure factor of di- and trinucleosomes from chicken erythrocytes and from COS-7 cells. We also determined the sedimentation coefficients of these dinucleosomes and dinucleosomes reconstituted on a 416-bp DNA containing two nucleosome positioning sequences of the 5S rDNA of Lytechinus variegatus at low and high salt concentrations. The internucleosomal distance d was calculated by simulation as well as Fourier back-transformation of the SANS curves and by hydrodynamic simulation of sedimentation coefficients. Nucleosome dimers from chicken erythrocyte chromatin show a decrease in d from approximately 220 A at 5 mM NaCl to 150 A at 100 mM NaCl. For dinucleosomes from COS-7 chromatin, d decreases from 180 A at 5 mM to 140 A at 100 mM NaCl concentration. Our measurements on trinucleosomes are compatible with a compaction through two different mechanisms, depending on the salt concentration. Between 0 and 20 mM NaCl, the internucleosomal distance between adjacent nucleosomes remains constant, whereas the angle of the DNA strands entering and leaving the central nucleosome decreases. Above 20 mM NaCl, the adjacent nucleosomes approach each other, similar to the compaction of dinucleosomes. The internucleosomal distance of 140-150 A at 100 mM NaCl is in agreement with distances measured by scanning force microscopy and electron microscopy on long chromatin filaments.
Bednar,
Chromatin conformation and salt-induced compaction: three-dimensional structural information from cryoelectron microscopy.
1995, Pubmed
Bednar,
Chromatin conformation and salt-induced compaction: three-dimensional structural information from cryoelectron microscopy.
1995,
Pubmed
Bednar,
Nucleosomes, linker DNA, and linker histone form a unique structural motif that directs the higher-order folding and compaction of chromatin.
1998,
Pubmed
Butler,
Dinucleosomes show compaction by ionic strength, consistent with bending of linker DNA.
1998,
Pubmed
Butler,
Changes in chromatin folding in solution.
1980,
Pubmed
Chahal,
Acetylation of histone H4 and its role in chromatin structure and function.
1980,
Pubmed
Dickerson,
Helix geometry and hydration in A-DNA, B-DNA, and Z-DNA.
1983,
Pubmed
Dong,
Nucleosome positioning is determined by the (H3-H4)2 tetramer.
1991,
Pubmed
Finch,
Solenoidal model for superstructure in chromatin.
1976,
Pubmed
Fletcher,
The nucleosomal array: structure/function relationships.
1996,
Pubmed
Garcia de la Torre,
HYDRO: a computer program for the prediction of hydrodynamic properties of macromolecules.
1994,
Pubmed
Gerchman,
Chromatin higher-order structure studied by neutron scattering and scanning transmission electron microscopy.
1987,
Pubmed
Godde,
Chromatin structure of Schizosaccharomyces pombe. A nucleosome repeat length that is shorter than the chromatosomal DNA length.
1992,
Pubmed
Graziano,
Histone H1 is located in the interior of the chromatin 30-nm filament.
1994,
Pubmed
Hagerman,
Flexibility of DNA.
1988,
Pubmed
Hamiche,
Linker histone-dependent DNA structure in linear mononucleosomes.
1996,
Pubmed
Hirai,
Interparticle interactions and structural changes of nucleosome core particles in low-salt solution.
1988,
Pubmed
Leuba,
Contributions of linker histones and histone H3 to chromatin structure: scanning force microscopy studies on trypsinized fibers.
1998,
Pubmed
Leuba,
Linker histone tails and N-tails of histone H3 are redundant: scanning force microscopy studies of reconstituted fibers.
1998,
Pubmed
Luger,
Crystal structure of the nucleosome core particle at 2.8 A resolution.
1997,
Pubmed
Luger,
The histone tails of the nucleosome.
1998,
Pubmed
Rydberg,
Chromatin conformation in living cells: support for a zig-zag model of the 30 nm chromatin fiber.
1998,
Pubmed
Simon,
A new procedure for purifying histone pairs H2A + H2B and H3 + H4 from chromatin using hydroxylapatite.
1979,
Pubmed
Simpson,
Chromatin reconstituted from tandemly repeated cloned DNA fragments and core histones: a model system for study of higher order structure.
1985,
Pubmed
,
Echinobase
Sperling,
The mass per unit length of chromatin by low-angle x-ray scattering.
1976,
Pubmed
Stafford,
Boundary analysis in sedimentation velocity experiments.
1994,
Pubmed
Stafford,
Sedimentation velocity spins a new weave for an old fabric.
1997,
Pubmed
Usachenko,
Alterations in nucleosome core structure in linker histone-depleted chromatin.
1996,
Pubmed
,
Echinobase
Varga-Weisz,
Analysis of modulators of chromatin structure in Drosophila.
1999,
Pubmed
Widom,
Structure, dynamics, and function of chromatin in vitro.
1998,
Pubmed
Woodcock,
Chromatin organization re-viewed.
1995,
Pubmed
Yao,
Linker DNA bending induced by the core histones of chromatin.
1991,
Pubmed
Yao,
Direct detection of linker DNA bending in defined-length oligomers of chromatin.
1990,
Pubmed
Zlatanova,
Chromatin fiber structure: morphology, molecular determinants, structural transitions.
1998,
Pubmed
van Holde,
Chromatin higher order structure: chasing a mirage?
1995,
Pubmed
van Holde,
What determines the folding of the chromatin fiber?
1996,
Pubmed