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Different Ca2+-releasing abilities of sperm extracts compared with tissue extracts and phospholipase C isoforms in sea urchin egg homogenate and mouse eggs.
Jones KT
,
Matsuda M
,
Parrington J
,
Katan M
,
Swann K
.
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A soluble phospholipase C (PLC) from boar sperm generates InsP(3) and hence causes Ca(2+) release when added to sea urchin egg homogenate. This PLC activity is associated with the ability of sperm extracts to cause Ca(2+) oscillations in mammalian eggs following fractionation. A sperm PLC may, therefore, be responsible for causing the observed Ca(2+) oscillations at fertilization. In the present study we have further characterized this boar sperm PLC activity using sea urchin egg homogenate. Consistent with a sperm PLC acting on egg PtdIns(4,5)P(2), the ability of sperm extracts to release Ca(2+) was blocked by preincubation with the PLC inhibitor U73122 or by the addition of neomycin to the homogenate. The Ca(2+)-releasing activity was also detectable in sperm from other species and in whole testis extracts. However, activity was not observed in extracts from other tissues. Moreover recombinant PLCbeta1, -gamma1, -gamma2, -delta1, all of which had higher specific activities than boar sperm extracts, were not able to release Ca(2+) in the sea urchin egg homogenate. In addition these PLCs were not able to cause Ca(2+) oscillations following microinjection into mouse eggs. These results imply that the sperm PLC possesses distinct properties that allow it to hydrolyse PtdIns(4,5)P(2) in eggs.
Allen,
Regulation of inositol lipid-specific phospholipase cdelta by changes in Ca2+ ion concentrations.
1997, Pubmed
Allen,
Regulation of inositol lipid-specific phospholipase cdelta by changes in Ca2+ ion concentrations.
1997,
Pubmed
Bleasdale,
Selective inhibition of receptor-coupled phospholipase C-dependent processes in human platelets and polymorphonuclear neutrophils.
1990,
Pubmed
Bootman,
The thiol reagent, thimerosal, evokes Ca2+ spikes in HeLa cells by sensitizing the inositol 1,4,5-trisphosphate receptor.
1992,
Pubmed
Busa,
An elevated free cytosolic Ca2+ wave follows fertilization in eggs of the frog, Xenopus laevis.
1985,
Pubmed
Ciapa,
Two phases of inositol polyphosphate and diacylglycerol production at fertilisation.
1986,
Pubmed
,
Echinobase
Clapper,
Inositol trisphosphate induces calcium release from nonmitochondrial stores i sea urchin egg homogenates.
1985,
Pubmed
,
Echinobase
Cuthbertson,
Phorbol ester and sperm activate mouse oocytes by inducing sustained oscillations in cell Ca2+.
1985,
Pubmed
Dupont,
Phospholipase C in mouse oocytes: characterization of beta and gamma isoforms and their possible involvement in sperm-induced Ca2+ spiking.
1996,
Pubmed
Eisen,
Temporal sequence and spatial distribution of early events of fertilization in single sea urchin eggs.
1984,
Pubmed
,
Echinobase
Ellis,
Catalytic domain of phosphoinositide-specific phospholipase C (PLC). Mutational analysis of residues within the active site and hydrophobic ridge of plcdelta1.
1998,
Pubmed
Evans,
Molecular mechanisms of sperm-egg interactions and egg activation.
1998,
Pubmed
Fissore,
Activation of development in mammals: is there a role for a sperm cytosolic factor?
1998,
Pubmed
Galione,
Ca(2+)-induced Ca2+ release in sea urchin egg homogenates: modulation by cyclic ADP-ribose.
1991,
Pubmed
,
Echinobase
Homa,
A cytosolic sperm factor triggers calcium oscillations and membrane hyperpolarizations in human oocytes.
1994,
Pubmed
Jaffe,
The path of calcium in cytosolic calcium oscillations: a unifying hypothesis.
1991,
Pubmed
Jin,
U73122 inhibits phospholipase C-dependent calcium mobilization in neuronal cells.
1994,
Pubmed
Jones,
The passage of Ca2+ and fluorescent markers between the sperm and egg after fusion in the mouse.
1998,
Pubmed
Jones,
A mammalian sperm cytosolic phospholipase C activity generates inositol trisphosphate and causes Ca2+ release in sea urchin egg homogenates.
1998,
Pubmed
,
Echinobase
Kimura,
Analysis of mouse oocyte activation suggests the involvement of sperm perinuclear material.
1998,
Pubmed
Kyozuka,
Injection of sperm extract mimics spatiotemporal dynamics of Ca2+ responses and progression of meiosis at fertilization of ascidian oocytes.
1998,
Pubmed
Lawrence,
Sperm-egg fusion is the prelude to the initial Ca2+ increase at fertilization in the mouse.
1997,
Pubmed
Lee,
A derivative of NADP mobilizes calcium stores insensitive to inositol trisphosphate and cyclic ADP-ribose.
1995,
Pubmed
,
Echinobase
Lee,
The calcium transient in sea urchin eggs during fertilization requires the production of inositol 1,4,5-trisphosphate.
1998,
Pubmed
,
Echinobase
Lee,
U73122 blocked the cGMP-induced calcium release in sea urchin eggs.
1998,
Pubmed
,
Echinobase
Lee,
Structural determination of a cyclic metabolite of NAD+ with intracellular Ca2+-mobilizing activity.
1989,
Pubmed
,
Echinobase
McCulloh,
Fusion of membranes during fertilization. Increases of the sea urchin egg's membrane capacitance and membrane conductance at the site of contact with the sperm.
1992,
Pubmed
,
Echinobase
Miyazaki,
Block of Ca2+ wave and Ca2+ oscillation by antibody to the inositol 1,4,5-trisphosphate receptor in fertilized hamster eggs.
1992,
Pubmed
Mogami,
Phospholipase C inhibitor, U73122, releases intracellular Ca2+, potentiates Ins(1,4,5)P3-mediated Ca2+ release and directly activates ion channels in mouse pancreatic acinar cells.
1997,
Pubmed
Nuccitelli,
How do sperm activate eggs?
1991,
Pubmed
Oda,
Spatiotemporal dynamics of the [Ca2+]i rise induced by microinjection of sperm extract into mouse eggs: preferential induction of a Ca2+ wave from the cortex mediated by the inositol 1,4,5-trisphosphate receptor.
1999,
Pubmed
Palermo,
Human sperm cytosolic factor triggers Ca2+ oscillations and overcomes activation failure of mammalian oocytes.
1997,
Pubmed
Parrington,
Calcium oscillations in mammalian eggs triggered by a soluble sperm protein.
1996,
Pubmed
Parrington,
The soluble sperm factor that causes Ca2+ release from sea-urchin (Lytechinus pictus) egg homogenates also triggers Ca2+ oscillations after injection into mouse eggs.
1999,
Pubmed
,
Echinobase
Paterson,
Phospholipase C delta 1 requires a pleckstrin homology domain for interaction with the plasma membrane.
1995,
Pubmed
Rhee,
Studies of inositol phospholipid-specific phospholipase C.
1989,
Pubmed
Sette,
Parthenogenetic activation of mouse eggs by microinjection of a truncated c-kit tyrosine kinase present in spermatozoa.
1997,
Pubmed
Sette,
Involvement of phospholipase Cgamma1 in mouse egg activation induced by a truncated form of the C-kit tyrosine kinase present in spermatozoa.
1998,
Pubmed
Shilling,
Evidence for both tyrosine kinase and G-protein-coupled pathways leading to starfish egg activation.
1994,
Pubmed
,
Echinobase
Speksnijder,
Free calcium pulses following fertilization in the ascidian egg.
1989,
Pubmed
Stith,
Inositol 1,4,5-trisphosphate mass changes from fertilization through first cleavage in Xenopus laevis.
1993,
Pubmed
Stricker,
Repetitive calcium waves induced by fertilization in the nemertean worm Cerebratulus lacteus.
1996,
Pubmed
Swann,
Different triggers for calcium oscillations in mouse eggs involve a ryanodine-sensitive calcium store.
1992,
Pubmed
Swann,
Dynamics of the calcium signal that triggers mammalian egg activation.
1994,
Pubmed
Swann,
The part played by inositol trisphosphate and calcium in the propagation of the fertilization wave in sea urchin eggs.
1986,
Pubmed
,
Echinobase
Swann,
A cytosolic sperm factor stimulates repetitive calcium increases and mimics fertilization in hamster eggs.
1990,
Pubmed
Takenawa,
Properties of phospholipase C isozymes.
1991,
Pubmed
Turner,
Fertilization increases the polyphosphoinositide content of sea urchin eggs.
1984,
Pubmed
,
Echinobase
Wang,
Interaction between ryanodine and neomycin binding sites on Ca2+ release channel from skeletal muscle sarcoplasmic reticulum.
1996,
Pubmed
Williams,
Binding of neomycin and calcium to phospholipids and other anionic compounds.
1986,
Pubmed
Wolosker,
Molecularly cloned mammalian glucosamine-6-phosphate deaminase localizes to transporting epithelium and lacks oscillin activity.
1998,
Pubmed
Wu,
Injection of a porcine sperm factor triggers calcium oscillations in mouse oocytes and bovine eggs.
1997,
Pubmed
Wu,
Partial characterization of the calcium-releasing activity of porcine sperm cytosolic extracts.
1998,
Pubmed
Yule,
U73122 inhibits Ca2+ oscillations in response to cholecystokinin and carbachol but not to JMV-180 in rat pancreatic acinar cells.
1992,
Pubmed