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Presence of invertebrate dystrophin-like products in obliquely striated muscle of the leech, Pontobdella muricata (Annelida, Hirudinea).
Royuela M
,
Paniagua R
,
Rivier F
,
Hugon G
,
Robert A
,
Mornet D
.
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Dystrophin is a 427-kDa cytoskeletal protein, which occurs in scant amounts in vertebrate muscle and nerve cells. No previous references to dystrophin or associated proteins in invertebrates at the protein level have been found, while two recent studies investigated the presence of genes encoding proteins homologous to dystrophin in sea urchin and other invertebrates such as Drosophila melanogaster. In this study, the possible presence and distribution of dystrophin-like proteins were studied in different invertebrate muscle cell types and species through Western blot analysis and light and electron microscope immunohistochemistry using a panel of antibodies whose specificities have been determined in vertebrates. Crude protein extracts of leech Pontobdella muricata were analysed by Western blotting. The revealed protein band, with 140 kDa molecular weight, was related to dystrophin, utrophin or dystrophin-related protein-2 (DRP2) according to the specificities of the antibodies used to detect them. The immunofluorescence study showed positive immunoreactions in obliquely striated muscle of this hyrudinean. The immunoelectron microscopy study confirmed specific immunogold labelling beneath the sarcolemma of muscle cells. We thus assume that this protein is an invertebrate dystrophin-like product that is referred to as IDLp140. The potential functions of this invertebrate dystrophin-like protein in invertebrate muscles are discussed relative to previous data in vertebrate tissues.
Ahn,
The three human syntrophin genes are expressed in diverse tissues, have distinct chromosomal locations, and each bind to dystrophin and its relatives.
1996, Pubmed
Ahn,
The three human syntrophin genes are expressed in diverse tissues, have distinct chromosomal locations, and each bind to dystrophin and its relatives.
1996,
Pubmed
Arahata,
Dystrophin and the membrane hypothesis of muscular dystrophy.
1989,
Pubmed
Campbell,
Three muscular dystrophies: loss of cytoskeleton-extracellular matrix linkage.
1995,
Pubmed
Cartaud,
Identification of dystrophin-binding protein(s) in membranes from Torpedo electrocyte and rat muscle.
1993,
Pubmed
Chang,
Dystrophin in electric organ of Torpedo californica homologous to that in human muscle.
1989,
Pubmed
Fabbrizio,
Dystrophin superfamily complexes are different in muscle and the nervous system.
1997,
Pubmed
Fabbrizio,
Re-evaluation of the distributions of dystrophin and utrophin in sciatic nerve.
1995,
Pubmed
Fabbrizio,
Monoclonal antibodies targeted against the C-terminal domain of dystrophin or utrophin.
1993,
Pubmed
Hoffman,
Cell and fiber-type distribution of dystrophin.
1988,
Pubmed
Labeit,
Evidence that nebulin is a protein-ruler in muscle thin filaments.
1991,
Pubmed
Lanzavecchia,
Superelongation in helical muscles of leeches.
1985,
Pubmed
Lidov,
Dp140: a novel 140 kDa CNS transcript from the dystrophin locus.
1995,
Pubmed
NORTH,
The fine structure of the myofibers in the heart of the snail Helix aspersa.
1963,
Pubmed
Nave,
A myofibrillar protein of insect muscle related to vertebrate titin connects Z band and A band: purification and molecular characterization of invertebrate mini-titin.
1990,
Pubmed
Ozawa,
From dystrophinopathy to sarcoglycanopathy: evolution of a concept of muscular dystrophy.
1998,
Pubmed
Paniagua,
Ultrastructure of invertebrate muscle cell types.
1996,
Pubmed
Petrof,
The molecular basis of activity-induced muscle injury in Duchenne muscular dystrophy.
1998,
Pubmed
Pons,
Isolated dystrophin molecules as seen by electron microscopy.
1990,
Pubmed
Pons,
Utrophin localization in normal and dystrophin-deficient heart.
1994,
Pubmed
Ravin,
Multiple dystrophin isoforms are associated with the postsynaptic membrane of Torpedo electric organ.
1991,
Pubmed
Rivier,
Presence of long and short dystrophin and/or utrophin products in Torpedo marmorata peripheral nerves.
1996,
Pubmed
Rivier,
Different utrophin and dystrophin properties related to their vascular smooth muscle distributions.
1997,
Pubmed
Roberts,
Characterization of DRP2, a novel human dystrophin homologue.
1996,
Pubmed
Roberts,
Dystrophins in vertebrates and invertebrates.
1998,
Pubmed
Royuela,
Immunocytochemical electron microscopic study and western blot analysis of myosin, paramyosin and miniparamyosin in the striated muscle of the fruit fly Drosophila melanogaster and in obliquely striated and smooth muscles of the earthworm Eisenia foetida.
1997,
Pubmed
Royuela,
Nebulin-like protein in the earthworm Eisenia foetida. Immunocytochemical electron microscopic study and western blot analysis of several muscle cell types.
1997,
Pubmed
Smith,
THE STRUCTURE OF INSECT FIBRILLAR FLIGHT MUSCLE : A Study Made with Special Reference to the Membrane Systems of the Fiber.
1961,
Pubmed
Vainzof,
The sarcoglycan complex in the six autosomal recessive limb-girdle muscular dystrophies.
1996,
Pubmed
Wang,
A sea urchin gene encoding dystrophin-related proteins.
1998,
Pubmed
,
Echinobase
Winder,
The membrane-cytoskeleton interface: the role of dystrophin and utrophin.
1997,
Pubmed
Yeadon,
Dystrophin is a component of the subsynaptic membrane.
1991,
Pubmed
Ziegler,
Titin-related proteins in invertebrate muscles.
1994,
Pubmed
de Eguileor,
Architecture of the T-system in helical paramyosin muscles of an annelid (Branchiobdella).
1980,
Pubmed