Anal Biochem 1999 Sep 10;2732:168-78. doi: 10.1006/abio.1999.4173.

Combined imaging and chemical sensing of fertilization-induced acid release from single sea urchin eggs.

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We demonstrate a microarray sensor capable of obtaining both chemical and visual information on multiple cells simultaneously with single-cell resolution. The array was fabricated by covalently immobilizing a thin, pH-sensitive polymer layer on the distal end of an optical imaging fiber. The sensor''s ability to measure localized chemical dynamics in real-time was evaluated using sea urchin fertilization biochemistry as a model system. Following sea urchin fertilization, the Na(+)/H(+) transporter is activated to exchange extracellular sodium ions for intracellular hydrogen ions, causing a release of hydrogen ions at the egg''s surface. By placing the pH sensor proximal to the egg and switching between a fluorescence image and a white light image, we were able to observe both localized pH changes following fertilization as well as morphological transformations during cell division.

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Genes referenced: LOC100887844 LOC115919910