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Dynein-ADP as a force-generating intermediate revealed by a rapid reactivation of flagellar axoneme.
Tani T
,
Kamimura S
.
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Fragmented flagellar axonemes of sand dollar spermatozoa were reactivated by rapid photolysis of caged ATP. After a time lag of 10 ms, axonemes treated with protease started sliding disintegration. Axonemes without protease digestion started nanometer-scale high-frequency oscillation after a similar time lag. Force development in the sliding disintegration was measured with a flexible glass needle and its time course was corresponded well to that of the dynein-ADP intermediate production estimated using kinetic rates previously reported. However, with a high concentration ( approximately 80 microM) of vanadate, which binds to the dynein-ADP intermediate and forms a stable complex of dynein-ADP-vanadate, the time course of force development in sliding disintegration was not affected at all. In the case of high frequency oscillation, the time lag to start the oscillation, the initial amplitude, and the initial frequency were not affected by vanadate, though the oscillation once started was damped more quickly at higher concentrations of vanadate. These results suggest that during the initial turnover of ATP hydrolysis, force generation of dynein is not blocked by vanadate. A vanadate-insensitive dynein-ADP is postulated as a force-generating intermediate.
Brokaw,
Mechanochemical coupling in flagella. I. Movement-dependent dephosphorylation of ATP by glycerinated spermatozoa.
1968, Pubmed
Brokaw,
Mechanochemical coupling in flagella. I. Movement-dependent dephosphorylation of ATP by glycerinated spermatozoa.
1968,
Pubmed
Brokaw,
Direct measurements of sliding between outer doublet microtubules in swimming sperm flagella.
1989,
Pubmed
,
Echinobase
Burgess,
Rigor and relaxed outer dynein arms in replicas of cryofixed motile flagella.
1995,
Pubmed
Gibbons,
Flagellar movement and adenosine triphosphatase activity in sea urchin sperm extracted with triton X-100.
1972,
Pubmed
,
Echinobase
Gibbons,
Acetate anions stabilize the latency of dynein 1 ATPase and increase the velocity of tubule sliding in reactivated sperm flagella.
1982,
Pubmed
,
Echinobase
Gibbons,
Multiple nucleotide-binding sites in the sequence of dynein beta heavy chain.
1991,
Pubmed
,
Echinobase
Gibbons,
Potent inhibition of dynein adenosinetriphosphatase and of the motility of cilia and sperm flagella by vanadate.
1978,
Pubmed
,
Echinobase
Gibbons,
Sliding and bending in sea urchin sperm flagella.
1982,
Pubmed
,
Echinobase
Gibbons,
Properties of flagellar "rigor waves" formed by abrupt removal of adenosine triphosphate from actively swimming sea urchin sperm.
1974,
Pubmed
,
Echinobase
Goldman,
Relaxation of muscle fibres by photolysis of caged ATP.
1982,
Pubmed
Goodenough,
Substructure of the outer dynein arm.
1982,
Pubmed
Goodno,
Inhibition of actomyosin ATPase by vanadate.
1982,
Pubmed
Hibberd,
Phosphate release and force generation in skeletal muscle fibers.
1985,
Pubmed
Higuchi,
Kinetics of force generation by single kinesin molecules activated by laser photolysis of caged ATP.
1997,
Pubmed
Holzbaur,
ADP release is rate limiting in steady-state turnover by the dynein adenosinetriphosphatase.
1989,
Pubmed
Holzbaur,
Microtubules accelerate ADP release by dynein.
1989,
Pubmed
Inaba,
Dynamic conformational changes of 21 S dynein ATPase coupled with ATP hydrolysis revealed by proteolytic digestion.
1989,
Pubmed
,
Echinobase
Inaba,
Anthraniloyl ATP, a fluorescent analog of ATP, as a substrate for dynein ATPase and flagellar motility.
1989,
Pubmed
,
Echinobase
Johnson,
The pathway of ATP hydrolysis by dynein. Kinetics of a presteady state phosphate burst.
1983,
Pubmed
Kamimura,
Direct measurement of the force of microtubule sliding in flagella.
1981,
Pubmed
,
Echinobase
Kamimura,
High-frequency nanometre-scale vibration in 'quiescent' flagellar axonemes.
1989,
Pubmed
,
Echinobase
Kamimura,
High-frequency vibration in flagellar axonemes with amplitudes reflecting the size of tubulin.
1992,
Pubmed
,
Echinobase
Kaplan,
Rapid photolytic release of adenosine 5'-triphosphate from a protected analogue: utilization by the Na:K pump of human red blood cell ghosts.
1978,
Pubmed
Lymn,
Mechanism of adenosine triphosphate hydrolysis by actomyosin.
1971,
Pubmed
McCray,
A new approach to time-resolved studies of ATP-requiring biological systems; laser flash photolysis of caged ATP.
1980,
Pubmed
Mocz,
Probing the nucleotide binding sites of axonemal dynein with the fluorescent nucleotide analogue 2'(3')-O-(-N-Methylanthraniloyl)-adenosine 5'-triphosphate.
1998,
Pubmed
,
Echinobase
Ogawa,
Four ATP-binding sites in the midregion of the beta heavy chain of dynein.
1991,
Pubmed
,
Echinobase
Oiwa,
The force-velocity relationship for microtubule sliding in demembranated sperm flagella of the sea urchin.
1988,
Pubmed
,
Echinobase
Okuno,
Inhibition and relaxation of sea urchin sperm flagella by vanadate.
1980,
Pubmed
,
Echinobase
Omoto,
Activation of the dynein adenosinetriphosphatase by microtubules.
1986,
Pubmed
Omoto,
Sea urchin axonemal motion supported by fluorescent, ribose-modified analogues of ATP.
1992,
Pubmed
,
Echinobase
Porter,
Transient state kinetic analysis of the ATP-induced dissociation of the dynein-microtubule complex.
1983,
Pubmed
Sale,
Study of the mechanism of vanadate inhibition of the dynein cross-bridge cycle in sea urchin sperm flagella.
1979,
Pubmed
,
Echinobase
Shimizu,
Steady-state kinetic study of vanadate-induced inhibition of ciliary dynein adenosinetriphosphatase activity from Tetrahymena.
1981,
Pubmed
Shimizu,
Presteady state kinetic analysis of vanadate-induced inhibition of the dynein ATPase.
1983,
Pubmed
Shimizu,
Activation of the dynein adenosinetriphosphatase by cross-linking to microtubules.
1989,
Pubmed
Shingyoji,
Cyclical bending movements induced locally by successive iontophoretic application of ATP to an elastase-treated flagellar axoneme.
1995,
Pubmed
,
Echinobase
Summers,
Adenosine triphosphate-induced sliding of tubules in trypsin-treated flagella of sea-urchin sperm.
1971,
Pubmed
,
Echinobase
Takahashi,
Microtubule sliding in reactivated flagella.
1982,
Pubmed
,
Echinobase
Tani,
Reactivation of sea-urchin sperm flagella induced by rapid photolysis of caged ATP.
1998,
Pubmed
,
Echinobase
Thirlwell,
Inhibition of unloaded shortening velocity in permeabilized muscle fibres by caged ATP compounds.
1995,
Pubmed
Tsukita,
ATP-dependent structural changes of the outer dynein arm in Tetrahymena cilia: a freeze-etch replica study.
1983,
Pubmed
Vale,
One-dimensional diffusion of microtubules bound to flagellar dynein.
1989,
Pubmed
,
Echinobase
Vale,
Different axoplasmic proteins generate movement in opposite directions along microtubules in vitro.
1985,
Pubmed
Witman,
Dynein arm conformation and mechanochemical transduction in the eukaryotic flagellum.
1982,
Pubmed
Yagi,
Nanometer scale vibration in mutant axonemes of Chlamydomonas.
1994,
Pubmed
,
Echinobase
Yano,
Sliding velocity between outer doublet microtubules of sea-urchin sperm axonemes.
1980,
Pubmed
,
Echinobase
