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Modeling electroporation in a single cell. I. Effects Of field strength and rest potential.
DeBruin KA
,
Krassowska W
.
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This study develops a model for a single cell electroporated by an external electric field and uses it to investigate the effects of shock strength and rest potential on the transmembrane potential V(m) and pore density N around the cell. As compared to the induced potential predicted by resistive-capacitive theory, the model of electroporation predicts a smaller magnitude of V(m) throughout the cell. Both V(m) and N are symmetric about the equator with the same value at both poles of the cell. Larger shocks do not increase the maximum magnitude of V(m) because more pores form to shunt the excess stimulus current across the membrane. In addition, the value of the rest potential does not affect V(m) around the cell because the electroporation current is several orders of magnitude larger than the ionic current that supports the rest potential. Once the field is removed, the shock-induced V(m) discharges within 2 micros, but the pores persist in the membrane for several seconds. Complete resealing to preshock conditions requires approximately 20 s. These results agree qualitatively and quantitatively with the experimental data reported by Kinosita and coworkers for unfertilized sea urchin eggs exposed to large electric fields.
Aguel,
Effects of electroporation on the transmembrane potential distribution in a two-dimensional bidomain model of cardiac tissue.
1999, Pubmed
Aguel,
Effects of electroporation on the transmembrane potential distribution in a two-dimensional bidomain model of cardiac tissue.
1999,
Pubmed
Barnett,
The current-voltage relation of an aqueous pore in a lipid bilayer membrane.
1990,
Pubmed
Benz,
Reversible electrical breakdown of lipid bilayer membranes: a charge-pulse relaxation study.
1979,
Pubmed
Benz,
Properties of the large ion-permeable pores formed from protein F of Pseudomonas aeruginosa in lipid bilayer membranes.
1981,
Pubmed
Chambers,
Membrane potential, action potential and activation potential of eggs of the sea urchin, Lytechinus variegatus.
1979,
Pubmed
,
Echinobase
DeBruin,
Electroporation and shock-induced transmembrane potential in a cardiac fiber during defibrillation strength shocks.
1998,
Pubmed
Djuzenova,
Effect of medium conductivity and composition on the uptake of propidium iodide into electropermeabilized myeloma cells.
1996,
Pubmed
Freeman,
Theory of electroporation of planar bilayer membranes: predictions of the aqueous area, change in capacitance, and pore-pore separation.
1994,
Pubmed
Gabriel,
Direct observation in the millisecond time range of fluorescent molecule asymmetrical interaction with the electropermeabilized cell membrane.
1997,
Pubmed
Genco,
Electroporation in symmetric and asymmetric membranes.
1993,
Pubmed
Glaser,
Reversible electrical breakdown of lipid bilayers: formation and evolution of pores.
1988,
Pubmed
Gross,
Optical imaging of cell membrane potential changes induced by applied electric fields.
1986,
Pubmed
Hibino,
Membrane conductance of an electroporated cell analyzed by submicrosecond imaging of transmembrane potential.
1991,
Pubmed
,
Echinobase
Hibino,
Time courses of cell electroporation as revealed by submicrosecond imaging of transmembrane potential.
1993,
Pubmed
,
Echinobase
Jones,
Microlesion formation in myocardial cells by high-intensity electric field stimulation.
1987,
Pubmed
Jones,
Response of cultured myocardial cells to countershock-type electric field stimulation.
1978,
Pubmed
Kinosita,
Voltage-induced conductance in human erythrocyte membranes.
1979,
Pubmed
Kinosita,
Dual-view microscopy with a single camera: real-time imaging of molecular orientations and calcium.
1991,
Pubmed
,
Echinobase
Kinosita,
Electroporation of cell membrane visualized under a pulsed-laser fluorescence microscope.
1988,
Pubmed
,
Echinobase
Knisley,
Asymmetrical electrically induced injury of rabbit ventricular myocytes.
1995,
Pubmed
Krassowska,
Effects of electroporation on transmembrane potential induced by defibrillation shocks.
1995,
Pubmed
Krassowska,
Response of a single cell to an external electric field.
1994,
Pubmed
Lojewska,
Analysis of the effect of medium and membrane conductance on the amplitude and kinetics of membrane potentials induced by externally applied electric fields.
1989,
Pubmed
Mehrle,
Electric pulse induced membrane permeabilization. Spatial orientation and kinetics of solute efflux in freely suspended and dielectrophoretically aligned plant mesophyll protoplasts.
1989,
Pubmed
Rosenberg,
Semiconductive properties of lipids and their possible relationship to lipid bilayer conductivity.
1968,
Pubmed
Rossignol,
Induction of calcium-dependent, localized cortical granule breakdown in sea-urchin eggs by voltage pulsation.
1983,
Pubmed
,
Echinobase
Tekle,
Selective and asymmetric molecular transport across electroporated cell membranes.
1994,
Pubmed
Tekle,
Electro-permeabilization of cell membranes: effect of the resting membrane potential.
1990,
Pubmed
Teruel,
Electroporation-induced formation of individual calcium entry sites in the cell body and processes of adherent cells.
1997,
Pubmed
Tovar,
Electroporation and recovery of cardiac cell membrane with rectangular voltage pulses.
1992,
Pubmed
Tsong,
Electroporation of cell membranes.
1991,
Pubmed
Tung,
Effects of strong electrical shock on cardiac muscle tissue.
1994,
Pubmed
Zhang,
Depth-targeted efficient gene delivery and expression in the skin by pulsed electric fields: an approach to gene therapy of skin aging and other diseases.
1996,
Pubmed
Zhou,
Transmembrane potential changes caused by shocks in guinea pig papillary muscle.
1996,
Pubmed
Zimmermann,
Electric field-mediated fusion and related electrical phenomena.
1982,
Pubmed
