Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
???displayArticle.abstract???
We report the use of a yeast one-hybrid system to isolate a transcriptional regulator of the sea urchin embryo hatching enzyme gene, SpHE. This gene is asymmetrically expressed along the animal-vegetal axis of sea urchin embryos under the cell-autonomous control of maternal regulatory activities and therefore provides an excellent entry point for understanding the mechanism that establishes animal-vegetal developmental polarity. To search for transcriptional regulators, we used a fragment of the SpHE promoter containing several individual elements instead of the conventional bait that contains a multimerized cis element. This screen yielded a number of positive clones that encode a new member of the Ets family, named SpEts4. This protein contains transcriptional activation activity, since expression of reporter genes in yeast does not depend on the presence of the yeast GAL4 activation domain. Sequences in the N-terminal region of SpEts4 mediate the activation activity, as shown by deletion or domain-swapping experiments. The newly identified DNA binding protein binds with a high degree of specificity to a SpHE promoter Ets element and forms a complex with a mobility identical to that obtained with 9-h sea urchin embryo nuclear extracts. SpEts4 positively regulates SpHE transcription, since mutation of the SpEts4 site in SpHE promoter transgenes reduces promoter activity in vivo while SpEts4 mRNA coinjection increases its output. As expected for a positive SpHE transcriptional regulator, the timing of SpEts4 gene expression precedes the transient expression of SpHE in the very early sea urchin blastula.
Ahne,
Characterization of a myeloid-specific enhancer of the chicken lysozyme gene. Major role for an Ets transcription factor-binding site.
1994, Pubmed
Ahne,
Characterization of a myeloid-specific enhancer of the chicken lysozyme gene. Major role for an Ets transcription factor-binding site.
1994,
Pubmed
Bassuk,
Physical interactions between Ets and NF-kappaB/NFAT proteins play an important role in their cooperative activation of the human immunodeficiency virus enhancer in T cells.
1997,
Pubmed
Boulikas,
A compilation and classification of DNA binding sites for protein transcription factors from vertebrates.
1994,
Pubmed
Buttice,
A polyomavirus enhancer A-binding protein-3 site and Ets-2 protein have a major role in the 12-O-tetradecanoylphorbol-13-acetate response of the human stromelysin gene.
1993,
Pubmed
Calzone,
Developmental appearance of factors that bind specifically to cis-regulatory sequences of a gene expressed in the sea urchin embryo.
1988,
Pubmed
,
Echinobase
Chen,
Molecular cloning of the ets proto-oncogene of the sea urchin and analysis of its developmental expression.
1988,
Pubmed
,
Echinobase
Chen,
Isolation and characterization of five Drosophila genes that encode an ets-related DNA binding domain.
1992,
Pubmed
Crepieux,
The Ets family of proteins: weak modulators of gene expression in quest for transcriptional partners.
1994,
Pubmed
Gan,
Repetitive DNA sequences linked to the sea urchin spec genes contain transcriptional enhancer-like elements.
1990,
Pubmed
,
Echinobase
Golub,
Oligomerization of the ABL tyrosine kinase by the Ets protein TEL in human leukemia.
1996,
Pubmed
Graves,
Specificity within the ets family of transcription factors.
1998,
Pubmed
Graves,
DNA binding by the ETS domain.
1996,
Pubmed
Gutman,
The collagenase gene promoter contains a TPA and oncogene-responsive unit encompassing the PEA3 and AP-1 binding sites.
1990,
Pubmed
John,
Importance of low affinity Elf-1 sites in the regulation of lymphoid-specific inducible gene expression.
1996,
Pubmed
Kim,
Isolation of a novel class of bZIP transcription factors that interact with ABA-responsive and embryo-specification elements in the Dc3 promoter using a modified yeast one-hybrid system.
1997,
Pubmed
Kozak,
The scanning model for translation: an update.
1989,
Pubmed
McMahon,
Introduction of cloned DNA into sea urchin egg cytoplasm: replication and persistence during embryogenesis.
1985,
Pubmed
,
Echinobase
Reynolds,
Early mRNAs, spatially restricted along the animal-vegetal axis of sea urchin embryos, include one encoding a protein related to tolloid and BMP-1.
1992,
Pubmed
,
Echinobase
Thompson,
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
1994,
Pubmed
Urness,
Molecular interactions within the ecdysone regulatory hierarchy: DNA binding properties of the Drosophila ecdysone-inducible E74A protein.
1990,
Pubmed
Wang,
Molecular cloning of the olfactory neuronal transcription factor Olf-1 by genetic selection in yeast.
1993,
Pubmed
Wasylyk,
The Ets family of transcription factors.
1993,
Pubmed
Wasylyk,
The c-Ets oncoprotein activates the stromelysin promoter through the same elements as several non-nuclear oncoproteins.
1991,
Pubmed
Wei,
Characterization of the SpHE promoter that is spatially regulated along the animal-vegetal axis of the sea urchin embryo.
1995,
Pubmed
,
Echinobase
Wei,
The SpHE gene is downregulated in sea urchin late blastulae despite persistence of multiple positive factors sufficient to activate its promoter.
1997,
Pubmed
,
Echinobase
Wei,
Multiple positive cis elements regulate the asymmetric expression of the SpHE gene along the sea urchin embryo animal-vegetal axis.
1997,
Pubmed
,
Echinobase
Wilson,
Identification of the DNA binding site for NGFI-B by genetic selection in yeast.
1991,
Pubmed
Yang,
Structure and tissue-specific developmental expression of a sea urchin arylsulfatase gene.
1989,
Pubmed
,
Echinobase
