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Mol Biol Cell
1998 Feb 01;92:333-43. doi: 10.1091/mbc.9.2.333.
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A specific light chain of kinesin associates with mitochondria in cultured cells.
Khodjakov A
,
Lizunova EM
,
Minin AA
,
Koonce MP
,
Gyoeva FK
.
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The motor protein kinesin is implicated in the intracellular transport of organelles along microtubules. Kinesin light chains (KLCs) have been suggested to mediate the selective binding of kinesin to its cargo. To test this hypothesis, we isolated KLC cDNA clones from a CHO-K1 expression library. Using sequence analysis, they were found to encode five distinct isoforms of KLCs. The primary region of variability lies at the carboxyl termini, which were identical or highly homologous to carboxyl-terminal regions of rat KLC B and C, human KLCs, sea urchin KLC isoforms 1-3, and squid KLCs. To examine whether the KLC isoforms associate with different cytoplasmic organelles, we made an antibody specific for a 10-amino acid sequence unique to B and C isoforms. In an indirect immunofluorescence assay, this antibody specifically labeled mitochondria in cultured CV-1 cells and human skin fibroblasts. On Western blots of total cell homogenates, it recognized a single KLC isoform, which copurified with mitochondria. Taken together, these data indicate a specific association of a particular KLC (B type) with mitochondria, revealing that different KLC isoforms can target kinesin to different cargoes.
Beushausen,
Kinesin light chains: identification and characterization of a family of proteins from the optic lobe of the squid Loligo pealii.
1993, Pubmed
Beushausen,
Kinesin light chains: identification and characterization of a family of proteins from the optic lobe of the squid Loligo pealii.
1993,
Pubmed
Bi,
Kinesin- and myosin-driven steps of vesicle recruitment for Ca2+-regulated exocytosis.
1997,
Pubmed
,
Echinobase
Bloom,
Native structure and physical properties of bovine brain kinesin and identification of the ATP-binding subunit polypeptide.
1988,
Pubmed
Bloom,
Motor proteins. 1: kinesins.
1994,
Pubmed
Cabeza-Arvelaiz,
Cloning and genetic characterization of the human kinesin light-chain (KLC) gene.
1993,
Pubmed
Cyr,
Molecular genetics of kinesin light chains: generation of isoforms by alternative splicing.
1991,
Pubmed
Fan,
Kinesin light chain isoforms in Caenorhabditis elegans.
1994,
Pubmed
Feiguin,
Kinesin-mediated organelle translocation revealed by specific cellular manipulations.
1994,
Pubmed
Gauger,
The Drosophila kinesin light chain. Primary structure and interaction with kinesin heavy chain.
1993,
Pubmed
Gindhart,
Tetratrico peptide repeats are present in the kinesin light chain.
1996,
Pubmed
Goldstein,
The kinesin superfamily: tails of functional redundancy.
1991,
Pubmed
Goodson,
Motors and membrane traffic.
1997,
Pubmed
Henson,
Immunolocalization of kinesin in sea urchin coelomocytes. Association of kinesin with intracellular organelles.
1992,
Pubmed
,
Echinobase
Hirokawa,
Submolecular domains of bovine brain kinesin identified by electron microscopy and monoclonal antibody decoration.
1989,
Pubmed
Hollenbeck,
Radial extension of macrophage tubular lysosomes supported by kinesin.
1990,
Pubmed
Hollenbeck,
Phosphorylation of neuronal kinesin heavy and light chains in vivo.
1993,
Pubmed
Hollenbeck,
The distribution, abundance and subcellular localization of kinesin.
1989,
Pubmed
Jellali,
Structural and biochemical properties of kinesin heavy chain associated with rat brain mitochondria.
1994,
Pubmed
Johnson,
Light chains of sea urchin kinesin identified by immunoadsorption.
1990,
Pubmed
,
Echinobase
Kuznetsov,
The quaternary structure of bovine brain kinesin.
1988,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Leopold,
Association of kinesin with characterized membrane-bounded organelles.
1992,
Pubmed
Matthies,
Calmodulin binding to and cAMP-dependent phosphorylation of kinesin light chains modulate kinesin ATPase activity.
1993,
Pubmed
Nakata,
Point mutation of adenosine triphosphate-binding motif generated rigor kinesin that selectively blocks anterograde lysosome membrane transport.
1995,
Pubmed
Nangaku,
KIF1B, a novel microtubule plus end-directed monomeric motor protein for transport of mitochondria.
1994,
Pubmed
Pereira,
Mitochondrial association of a plus end-directed microtubule motor expressed during mitosis in Drosophila.
1997,
Pubmed
Pfister,
Monoclonal antibodies to kinesin heavy and light chains stain vesicle-like structures, but not microtubules, in cultured cells.
1989,
Pubmed
Rodionov,
Microtubule-dependent control of cell shape and pseudopodial activity is inhibited by the antibody to kinesin motor domain.
1993,
Pubmed
Schmitz,
Intracellular distribution of kinesin in chromaffin cells.
1994,
Pubmed
Scholey,
Kinesin-II, a membrane traffic motor in axons, axonemes, and spindles.
1996,
Pubmed
Scholey,
Identification of globular mechanochemical heads of kinesin.
1989,
Pubmed
Seiler,
Kinesin is essential for cell morphogenesis and polarized secretion in Neurospora crassa.
1997,
Pubmed
Skoufias,
The carboxyl-terminal domain of kinesin heavy chain is important for membrane binding.
1994,
Pubmed
,
Echinobase
Steinberg,
The Neurospora organelle motor: a distant relative of conventional kinesin with unconventional properties.
1995,
Pubmed
Stenoien,
Immunochemical analysis of kinesin light chain function.
1997,
Pubmed
Wagner,
Copurification of kinesin polypeptides with microtubule-stimulated Mg-ATPase activity and kinetic analysis of enzymatic properties.
1989,
Pubmed
Wedaman,
Sequences of sea urchin kinesin light chain isoforms.
1993,
Pubmed
,
Echinobase
Wright,
Subcellular localization and sequence of sea urchin kinesin heavy chain: evidence for its association with membranes in the mitotic apparatus and interphase cytoplasm.
1991,
Pubmed
,
Echinobase
Yang,
A three-domain structure of kinesin heavy chain revealed by DNA sequence and microtubule binding analyses.
1989,
Pubmed