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A gene (NhKIN1) encoding a kinesin was cloned from Nectria haematococca genomic DNA by polymerase chain reaction amplification, using primers corresponding to conserved regions of known kinesin-encoding genes. Sequence analysis showed that NhKIN1 belongs to the subfamily of conventional kinesins and is distinct from any of the currently designated kinesin-related protein subfamilies. Deletion of NhKIN1 by transformation-mediated homologous recombination caused several dramatic phenotypes: a 50% reduction in colony growth rate, helical or wavy hyphae with reduced diameter, and subcellular abnormalities including withdrawal of mitochondria from the growing hyphal apex and reduction in the size of the Spitzenkörper, an apical aggregate of secretory vesicles. The effects on mitochondria and Spitzenkörper were not due to altered microtubule distribution, as microtubules were abundant throughout the length of hyphal tip cells of the mutant. The rate of spindle elongation during anaphase B of mitosis was reduced 11%, but the rate was not significantly different from that of wild type. This lack of a substantial mitotic phenotype is consistent with the primary role of the conventional kinesins in organelle motility rather than mitosis. Our results provide further evidence that the microtubule-based motility mechanism has a direct role in apical transport of secretory vesicles and the first evidence for its role in apical transport of mitochondria in a filamentous fungus. They also include a unique demonstration that a microtubule-based motor protein is essential for normal positioning of the Spitzenkörper, thus providing a new insight into the cellular basis for the aberrant hyphal morphology.
Aist,
Direct experimental evidence for the existence, structural basis and function of astral forces during anaphase B in vivo.
1991, Pubmed,
Echinobase
Aist,
Direct experimental evidence for the existence, structural basis and function of astral forces during anaphase B in vivo.
1991,
Pubmed
,
Echinobase
Allan,
Membrane traffic motors.
1995,
Pubmed
Allen,
Effects of cytochalasins on Neurospora crassa. I. Growth and ultrastructure.
1980,
Pubmed
Bairoch,
PROSITE: a dictionary of sites and patterns in proteins.
1992,
Pubmed
Bartnicki-Garcia,
Evidence that Spitzenkörper behavior determines the shape of a fungal hypha: a test of the hyphoid model.
1995,
Pubmed
Barton,
Going mobile: microtubule motors and chromosome segregation.
1996,
Pubmed
Baumann,
Microtubule-associated movement of mitochondria and small particles in Acanthamoeba castellanii.
1995,
Pubmed
Bloom,
Motor proteins 1: kinesins.
1995,
Pubmed
Brady,
A kinesin medley: biochemical and functional heterogeneity.
1995,
Pubmed
Cole,
Organization of organelles and membrane traffic by microtubules.
1995,
Pubmed
Goldstein,
Functional redundancy in mitotic force generation.
1993,
Pubmed
Grove,
Protoplasmic organization of hyphal tips among fungi: vesicles and Spitzenkörper.
1970,
Pubmed
Hirokawa,
Organelle transport along microtubules - the role of KIFs.
1996,
Pubmed
Howard,
Ultrastructural analysis of hyphal tip cell growth in fungi: Spitzenkörper, cytoskeleton and endomembranes after freeze-substitution.
1981,
Pubmed
Howard,
Effects of MBC on hyphal tip organization, growth, and mitosis of Fusarium acuminatum, and their antagonism by D2O.
1977,
Pubmed
Howard,
Cytoplasmic microtubules and fungal morphogenesis: ultrastructural effects of methyl benzimidazole-2-ylcarbamate determined by freeze-substitution of hyphal tip cells.
1980,
Pubmed
Hoyt,
Cellular roles of kinesin and related proteins.
1994,
Pubmed
Hurst,
Transcription factors 1: bZIP proteins.
1995,
Pubmed
Jellali,
Structural and biochemical properties of kinesin heavy chain associated with rat brain mitochondria.
1994,
Pubmed
Lehmler,
Identification of a motor protein required for filamentous growth in Ustilago maydis.
1997,
Pubmed
Leopold,
Association of kinesin with characterized membrane-bounded organelles.
1992,
Pubmed
Li,
Disruption of mitotic spindle orientation in a yeast dynein mutant.
1993,
Pubmed
,
Echinobase
Lillie,
Suppression of a myosin defect by a kinesin-related gene.
1992,
Pubmed
Logemann,
Improved method for the isolation of RNA from plant tissues.
1987,
Pubmed
McGoldrick,
myoA of Aspergillus nidulans encodes an essential myosin I required for secretion and polarized growth.
1995,
Pubmed
Meluh,
KAR3, a kinesin-related gene required for yeast nuclear fusion.
1990,
Pubmed
Messing,
New M13 vectors for cloning.
1983,
Pubmed
Moore,
Kinesin proteins: a phylum of motors for microtubule-based motility.
1996,
Pubmed
Morris,
Axonal transport of mitochondria along microtubules and F-actin in living vertebrate neurons.
1995,
Pubmed
Nangaku,
KIF1B, a novel microtubule plus end-directed monomeric motor protein for transport of mitochondria.
1994,
Pubmed
Navone,
Cloning and expression of a human kinesin heavy chain gene: interaction of the COOH-terminal domain with cytoplasmic microtubules in transfected CV-1 cells.
1992,
Pubmed
Novick,
Phenotypic analysis of temperature-sensitive yeast actin mutants.
1985,
Pubmed
Patel,
Cloning by insertional mutagenesis of a cDNA encoding Caenorhabditis elegans kinesin heavy chain.
1993,
Pubmed
Plamann,
Cytoplasmic dynein and actin-related protein Arp1 are required for normal nuclear distribution in filamentous fungi.
1994,
Pubmed
Raudaskoski,
Microtubule cytoskeleton in hyphal growth. Response to nocodazole in a sensitive and a tolerant strain of the homobasidiomycete Schizophyllum commune.
1994,
Pubmed
Reynolds,
RAD6 gene of Saccharomyces cerevisiae encodes a protein containing a tract of 13 consecutive aspartates.
1985,
Pubmed
Rodionov,
Microtubule-dependent control of cell shape and pseudopodial activity is inhibited by the antibody to kinesin motor domain.
1993,
Pubmed
Rossier,
Secretion of an Mr 60000 protein by benomyl-treated cells of Neurospora crassa.
1989,
Pubmed
Saraste,
The P-loop--a common motif in ATP- and GTP-binding proteins.
1990,
Pubmed
Saunders,
Mitotic spindle pole separation.
1993,
Pubmed
Seiler,
Kinesin is essential for cell morphogenesis and polarized secretion in Neurospora crassa.
1997,
Pubmed
Sherwood-Higham,
Helical growth of hyphae of Candida albicans.
1994,
Pubmed
Simon,
Actin-dependent mitochondrial motility in mitotic yeast and cell-free systems: identification of a motor activity on the mitochondrial surface.
1995,
Pubmed
Soshi,
The mus-8 gene of Neurospora crassa encodes a structural and functional homolog of the Rad6 protein of Saccharomyces cerevisiae.
1996,
Pubmed
Steele,
Effect of temperature and temperature shifts on growth and branching of a wild type and a temperature sensitive colonial mutant (Cot 1) of Neurospora crassa.
1977,
Pubmed
Steinberg,
Organelle movements in the wild type and wall-less fz;sg;os-1 mutants of Neurospora crassa are mediated by cytoplasmic microtubules.
1993,
Pubmed
Steinberg,
The Neurospora organelle motor: a distant relative of conventional kinesin with unconventional properties.
1995,
Pubmed
Sullivan,
Cloning of a 16-kDa ubiquitin carrier protein from wheat and Arabidopsis thaliana. Identification of functional domains by in vitro mutagenesis.
1991,
Pubmed
Turgeon,
Development of a fungal transformation system based on selection of sequences with promoter activity.
1987,
Pubmed
Turgeon,
Cloning and analysis of the mating type genes from Cochliobolus heterostrophus.
1993,
Pubmed
Tzeng,
A restriction fragment length polymorphism map and electrophoretic karyotype of the fungal maize pathogen Cochliobolus heterostrophus.
1992,
Pubmed
Vallee,
Targeting of motor proteins.
1996,
Pubmed
Wu,
A fungal kinesin required for organelle motility, hyphal growth, and morphogenesis.
1998,
Pubmed
,
Echinobase
Xiang,
Characterization and localization of the cytoplasmic dynein heavy chain in Aspergillus nidulans.
1995,
Pubmed
Yaffe,
Microtubules mediate mitochondrial distribution in fission yeast.
1996,
Pubmed
Yang,
A three-domain structure of kinesin heavy chain revealed by DNA sequence and microtubule binding analyses.
1989,
Pubmed
