Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
J Bacteriol
1997 Dec 01;17923:7321-30. doi: 10.1128/jb.179.23.7321-7330.1997.
Show Gene links
Show Anatomy links
Pectate lyase PelI of Erwinia chrysanthemi 3937 belongs to a new family.
Shevchik VE
,
Robert-Baudouy J
,
Hugouvieux-Cotte-Pattat N
.
???displayArticle.abstract???
Erwinia chrysanthemi 3937 secretes five major isoenzymes of pectate lyases encoded by the pel4, pelB, pelC, pelD, and pelE genes and a set of secondary pectate lyases, two of which, pelL and pelZ, have been already identified. We cloned the pelI gene, encoding a ninth pectate lyase of E. chrysanthemi 3937. The pelI reading frame is 1,035 bases long, corresponding to a protein of 344 amino acids including a typical amino-terminal signal sequence of 19 amino acids. The purified mature PelI protein has an isoelectric point of about 9 and an apparent molecular mass of 34 kDa. PelI has a preference for partially methyl esterified pectin and presents an endo-cleaving activity with an alkaline pH optimum and an absolute requirement for Ca2+ ions. PelI is an extracellular protein secreted by the Out secretory pathway of E. chrysanthemi. The PelI protein is very active in the maceration of plant tissues. A pelI mutant displayed reduced pathogenicity on chicory leaves, but its virulence did not appear to be affected on potato tubers or Saintpaulia ionantha plants. The pelI gene constitutes an independent transcriptional unit. As shown for the other pel genes, the transcription of pelI is dependent on various environmental conditions. It is induced by pectic catabolic products and affected by growth phase, oxygen limitation, temperature, nitrogen starvation, and catabolite repression. Regulation of pelI expression appeared to be dependent on the three repressors of pectinase synthesis, KdgR, PecS, and PecT, and on the global activator of sugar catabolism, cyclic AMP receptor protein. A functional KdgR binding site was identified close to the putative pelI promoter. Analysis of the amino acid sequence of PelI revealed high homology with a pectate lyase from Erwinia carotovora subsp. carotovora (65% identity) and low homology with pectate lyases of the phytopathogenic fungus Nectria haematococca (Fusarium solani). This finding indicates that PelI belongs to pectate lyase class III. Using immunoblotting experiments, we detected PelI homologs in various strains of E. chrysanthemi and E. carotovora subsp. carotovora but not in E. carotovora subsp. atroseptica.
Alfano,
Use of Tn5tac1 to clone a pel gene encoding a highly alkaline, asparagine-rich pectate lyase isozyme from an Erwinia chrysanthemi EC16 mutant with deletions affecting the major pectate lyase isozymes.
1995, Pubmed
Alfano,
Use of Tn5tac1 to clone a pel gene encoding a highly alkaline, asparagine-rich pectate lyase isozyme from an Erwinia chrysanthemi EC16 mutant with deletions affecting the major pectate lyase isozymes.
1995,
Pubmed
Bardonnet,
'uidA-antibiotic-resistance cassettes for insertion mutagenesis, gene fusions and genetic constructions.
1992,
Pubmed
Barras,
Resolution of four pectate lyase structural genes of Erwinia chrysanthemi (EC16) and characterization of the enzymes produced in Escherichia coli.
1987,
Pubmed
Bertheau,
Detection of depolymerase isoenzymes after electrophoresis or electrofocusing, or in titration curves.
1984,
Pubmed
Brooks,
Molecular cloning of the structural gene for exopolygalacturonate lyase from Erwinia chrysanthemi EC16 and characterization of the enzyme product.
1990,
Pubmed
Condemine,
Analysis of an Erwinia chrysanthemi gene cluster involved in pectin degradation.
1991,
Pubmed
Copeland,
Renaturation and identification of periplasmic proteins in two-dimensional gels of Escherichia coli.
1982,
Pubmed
Expert,
Bacteriocin-resistant mutants of Erwinia chrysanthemi: possible involvement of iron acquisition in phytopathogenicity.
1985,
Pubmed
Gijsegem,
In vivo cloning of the pectate lyase and cellulase genes of Erwinia chrysanthemi.
1985,
Pubmed
González-Candelas,
Isolation and analysis of a novel inducible pectate lyase gene from the phytopathogenic fungus Fusarium solani f. sp. pisi (Nectria haematococca, mating population VI).
1992,
Pubmed
,
Echinobase
Guo,
Cloning of a novel constitutively expressed pectate lyase gene pelB from Fusarium solani f. sp. pisi (Nectria haematococca, mating type VI) and characterization of the gene product expressed in Pichia pastoris.
1995,
Pubmed
,
Echinobase
Guo,
Cloning of a new pectate lyase gene pelC from Fusarium solani f. sp. pisi (Nectria haematococca, mating type VI) and characterization of the gene product expressed in Pichia pastoris.
1995,
Pubmed
,
Echinobase
Heikinheimo,
Characterization of a novel pectate lyase from Erwinia carotovora subsp. carotovora.
1995,
Pubmed
Henrissat,
Functional implications of structure-based sequence alignment of proteins in the extracellular pectate lyase superfamily.
1995,
Pubmed
Hinton,
Extracellular and periplasmic isoenzymes of pectate lyase from Erwinia carotovora subspecies carotovora belong to different gene families.
1989,
Pubmed
Hugouvieux-Cotte-Pattat,
Analysis of the regulation of the pelBC genes in Erwinia chrysanthemi 3937.
1992,
Pubmed
Hugouvieux-Cotte-Pattat,
Environmental conditions affect transcription of the pectinase genes of Erwinia chrysanthemi 3937.
1992,
Pubmed
Hugouvieux-Cotte-Pattat,
Regulation of pectinolysis in Erwinia chrysanthemi.
1996,
Pubmed
Hugouvieux-Cotte-Pattat,
Isolation of Erwinia chrysanthemi mutants altered in pectinolytic enzyme production.
1989,
Pubmed
Johnson,
Recombinant proteins can be isolated from E. coli cells by repeated cycles of freezing and thawing.
1994,
Pubmed
Kelemu,
Erwinia chrysanthemi EC16 Produces a Second Set of Plant-Inducible Pectate Lyase Isozymes.
1993,
Pubmed
Liu,
Nucleotide sequence and expression of a novel pectate lyase gene (pel-3) and a closely linked endopolygalacturonase gene (peh-1) of Erwinia carotovora subsp. carotovora 71.
1994,
Pubmed
Lojkowska,
Characterization of the pelL gene encoding a novel pectate lyase of Erwinia chrysanthemi 3937.
1995,
Pubmed
Maes,
Phenol as grinding material in RNA preparations.
1992,
Pubmed
Moran,
Extracellular and intracellular polygllacturonic acid trans-eliminases of Erwinia carotovora.
1968,
Pubmed
Nasser,
Specific interactions of Erwinia chrysanthemi KdgR repressor with different operators of genes involved in pectinolysis.
1994,
Pubmed
Pissavin,
Regulation of pelZ, a gene of the pelB-pelC cluster encoding a new pectate lyase of Erwinia chrysanthemi 3937.
1996,
Pubmed
Reverchon,
pecS: a locus controlling pectinase, cellulase and blue pigment production in Erwinia chrysanthemi.
1994,
Pubmed
Reverchon,
The cyclic AMP receptor protein is the main activator of pectinolysis genes in Erwinia chrysanthemi.
1997,
Pubmed
Reverchon,
Characterization of kdgR, a gene of Erwinia chrysanthemi that regulates pectin degradation.
1991,
Pubmed
Roeder,
Marker-exchange mutagenesis of a pectate lyase isozyme gene in Erwinia chrysanthemi.
1985,
Pubmed
Shevchik,
Identification of a bacterial pectin acetyl esterase in Erwinia chrysanthemi 3937.
1997,
Pubmed
Studier,
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.
1986,
Pubmed
Surgey,
The Erwinia chrysanthemi pecT gene regulates pectinase gene expression.
1996,
Pubmed
Tabor,
A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.
1985,
Pubmed
Tardy,
Comparative analysis of the five major Erwinia chrysanthemi pectate lyases: enzyme characteristics and potential inhibitors.
1997,
Pubmed
Yoder,
Unusual structural features in the parallel beta-helix in pectate lyases.
1993,
Pubmed
Yoder,
New domain motif: the structure of pectate lyase C, a secreted plant virulence factor.
1993,
Pubmed