Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Mary J
,
Redeker V
,
Le Caer JP
,
Rossier J
,
Schmitter JM
.
???displayArticle.abstract??? Axonemal tubulin exhibits a high degree of heterogeneity mostly due to several posttranslational modifications (PTM). The aim of this work was to chemically characterize the different PTM occurring in the C-terminal tail of axonemaltubulin purified from sea urchin, Paracentrotus lividus, spermatozoa. After its purification, tubulin was enzymatically cleaved. The C-terminal peptides were chromatographically isolated, first by anion exchange and then by reverse-phase HPLC. Peptides were characterized by their sequence, determined by Edman degradation, and by their mass, determined by MALDI-TOF/MS. The two major conclusions are that the majority of the isolated C-terminal peptides were unmodified and that polyglycylation and polyglutamylation can occur simultaneously on one molecule of alpha-tubulin.
Barra,
A soluble preparation from rat brain that incorporates into its own proteins ( 14 C)arginine by a ribonuclease-sensitive system and ( 14 C)tyrosine by a ribonuclease-insensitive system.
1973, Pubmed
Barra,
A soluble preparation from rat brain that incorporates into its own proteins ( 14 C)arginine by a ribonuclease-sensitive system and ( 14 C)tyrosine by a ribonuclease-insensitive system.
1973,
Pubmed
Binder,
The in vitro assembly of flagellar outer doublet tubulin.
1978,
Pubmed
Bré,
Axonemal tubulin polyglycylation probed with two monoclonal antibodies: widespread evolutionary distribution, appearance during spermatozoan maturation and possible function in motility.
1996,
Pubmed
,
Echinobase
Díaz-Nido,
Phosphorylation of a neuronal-specific beta-tubulin isotype.
1990,
Pubmed
Eddé,
Posttranslational glutamylation of alpha-tubulin.
1990,
Pubmed
Gagnon,
The polyglutamylated lateral chain of alpha-tubulin plays a key role in flagellar motility.
1996,
Pubmed
,
Echinobase
Geuens,
Microtubule dynamics investigated by microinjection of Paramecium axonemal tubulin: lack of nucleation but proximal assembly of microtubules at the kinetochore during prometaphase.
1989,
Pubmed
LeDizet,
Identification of an acetylation site of Chlamydomonas alpha-tubulin.
1987,
Pubmed
Ludueña,
Identification of the phosphorylated beta-tubulin isotype in differentiated neuroblastoma cells.
1988,
Pubmed
Mary,
Posttranslational modifications in the C-terminal tail of axonemal tubulin from sea urchin sperm.
1996,
Pubmed
,
Echinobase
Paturle-Lafanechère,
Characterization of a major brain tubulin variant which cannot be tyrosinated.
1991,
Pubmed
Redeker,
Polyglycylation of tubulin: a posttranslational modification in axonemal microtubules.
1994,
Pubmed
Sullivan,
Structure and utilization of tubulin isotypes.
1988,
Pubmed
Wolff,
High level of tubulin microheterogeneity in the mouse brain.
1982,
Pubmed