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ECB-ART-36689
Mol Biol Cell 1997 Jul 01;87:1207-17. doi: 10.1091/mbc.8.7.1207.
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The cdk7-cyclin H-MAT1 complex associated with TFIIH is localized in coiled bodies.

Jordan P , Cunha C , Carmo-Fonseca M .


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TFIIH is a general transcription factor for RNA polymerase II that in addition is involved in DNA excision repair. TFIIH is composed of eight or nine subunits and we show that at least four of them, namely cdk7, cyclin H, MAT1, and p62 are localized in the coiled body, a distinct subnuclear structure that is transcription dependent and highly enriched in small nuclear ribonucleoproteins. Although coiled bodies do not correspond to sites of transcription, in vivo incorporation of bromo-UTP shows that they are surrounded by transcription foci. Immunofluorescence analysis using antibodies directed against the essential repair factors proliferating cell nuclear antigen and XPG did not reveal labeling of the coiled body in either untreated cells or cells irradiated with UV light, arguing that coiled bodies are probably not involved in DNA repair mechanisms. The localization of cyclin H in the coiled body was predominantly detected during the G1 and S-phases of the cell cycle, whereas in G2 coiled bodies were very small or not detected. Finally, both cyclin H and cdk7 did not colocalize with P80 coilin after disruption of the coiled body, indicating that these proteins are specifically targeted to the small nuclear ribonucleoprotein-containing domain.

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Genes referenced: btaf1 LOC105441174 LOC115919910 LOC587097 LOC765052 mnat1 polr3a

References [+] :
Adamczewski, MAT1, cdk7 and cyclin H form a kinase complex which is UV light-sensitive upon association with TFIIH. 1996, Pubmed