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The cdk7-cyclin H-MAT1 complex associated with TFIIH is localized in coiled bodies.
Jordan P
,
Cunha C
,
Carmo-Fonseca M
.
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TFIIH is a general transcription factor for RNA polymerase II that in addition is involved in DNA excision repair. TFIIH is composed of eight or nine subunits and we show that at least four of them, namely cdk7, cyclin H, MAT1, and p62 are localized in the coiled body, a distinct subnuclear structure that is transcription dependent and highly enriched in small nuclear ribonucleoproteins. Although coiled bodies do not correspond to sites of transcription, in vivo incorporation of bromo-UTP shows that they are surrounded by transcription foci. Immunofluorescence analysis using antibodies directed against the essential repair factors proliferating cell nuclear antigen and XPG did not reveal labeling of the coiled body in either untreated cells or cells irradiated with UV light, arguing that coiled bodies are probably not involved in DNA repair mechanisms. The localization of cyclin H in the coiled body was predominantly detected during the G1 and S-phases of the cell cycle, whereas in G2 coiled bodies were very small or not detected. Finally, both cyclin H and cdk7 did not colocalize with P80 coilin after disruption of the coiled body, indicating that these proteins are specifically targeted to the small nuclear ribonucleoprotein-containing domain.
Adamczewski,
MAT1, cdk7 and cyclin H form a kinase complex which is UV light-sensitive upon association with TFIIH.
1996, Pubmed
Adamczewski,
MAT1, cdk7 and cyclin H form a kinase complex which is UV light-sensitive upon association with TFIIH.
1996,
Pubmed
Andrade,
Immunocytochemical analysis of the coiled body in the cell cycle and during cell proliferation.
1993,
Pubmed
Andrade,
Human autoantibody to a novel protein of the nuclear coiled body: immunological characterization and cDNA cloning of p80-coilin.
1991,
Pubmed
Bauer,
In vitro assembly of coiled bodies in Xenopus egg extract.
1994,
Pubmed
Bentley,
Regulation of transcriptional elongation by RNA polymerase II.
1995,
Pubmed
Blangy,
Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo.
1995,
Pubmed
Bohmann,
Mutational analysis of p80 coilin indicates a functional interaction between coiled bodies and the nucleolus.
1995,
Pubmed
Bohmann,
Molecular analysis of the coiled body.
1995,
Pubmed
Bravo,
Existence of two populations of cyclin/proliferating cell nuclear antigen during the cell cycle: association with DNA replication sites.
1987,
Pubmed
Carmo-Fonseca,
Assembly of snRNP-containing coiled bodies is regulated in interphase and mitosis--evidence that the coiled body is a kinetic nuclear structure.
1993,
Pubmed
Carmo-Fonseca,
The topography of chromosomes and genes in the nucleus.
1996,
Pubmed
Drapkin,
Human cyclin-dependent kinase-activating kinase exists in three distinct complexes.
1996,
Pubmed
Du,
A functional interaction between the carboxy-terminal domain of RNA polymerase II and pre-mRNA splicing.
1997,
Pubmed
Fakan,
Perichromatin fibrils are in situ forms of nascent transcripts.
1994,
Pubmed
Ferreira,
Differential interaction of splicing snRNPs with coiled bodies and interchromatin granules during mitosis and assembly of daughter cell nuclei.
1994,
Pubmed
Ferreira,
The biogenesis of the coiled body during early mouse development.
1995,
Pubmed
Fey,
The nonchromatin substructures of the nucleus: the ribonucleoprotein (RNP)-containing and RNP-depleted matrices analyzed by sequential fractionation and resinless section electron microscopy.
1986,
Pubmed
Fischer,
Cloning of the 62-kilodalton component of basic transcription factor BTF2.
1992,
Pubmed
Frey,
Coiled bodies contain U7 small nuclear RNA and associate with specific DNA sequences in interphase human cells.
1995,
Pubmed
Friedberg,
Relationships between DNA repair and transcription.
1996,
Pubmed
Hoeijmakers,
TFIIH: a key component in multiple DNA transactions.
1996,
Pubmed
Jackson,
Sites in human nuclei where damage induced by ultraviolet light is repaired: localization relative to transcription sites and concentrations of proliferating cell nuclear antigen and the tumour suppressor protein, p53.
1994,
Pubmed
Jackson,
Visualization of focal sites of transcription within human nuclei.
1993,
Pubmed
Jiménez-García,
Nucleologenesis: U3 snRNA-containing prenucleolar bodies move to sites of active pre-rRNA transcription after mitosis.
1994,
Pubmed
Jiménez-García,
Organization of the double-stranded RNA-activated protein kinase DAI and virus-associated VA RNAI in adenovirus-2-infected HeLa cells.
1993,
Pubmed
Jordan,
In vivo evidence that TATA-binding protein/SL1 colocalizes with UBF and RNA polymerase I when rRNA synthesis is either active or inactive.
1996,
Pubmed
Lamond,
The coiled body.
1993,
Pubmed
Landberg,
Antibodies to proliferating cell nuclear antigen as S-phase probes in flow cytometric cell cycle analysis.
1991,
Pubmed
Linné,
Purification and characterization of the phosphorylated DNA-binding protein from adenovirus-type-2-infected cells.
1977,
Pubmed
Maldonado,
News on initiation and elongation of transcription by RNA polymerase II.
1995,
Pubmed
McCracken,
The C-terminal domain of RNA polymerase II couples mRNA processing to transcription.
1997,
Pubmed
Meier,
NAP57, a mammalian nucleolar protein with a putative homolog in yeast and bacteria.
1994,
Pubmed
Morgan,
Principles of CDK regulation.
1995,
Pubmed
Nigg,
Cyclin-dependent protein kinases: key regulators of the eukaryotic cell cycle.
1995,
Pubmed
Nigg,
Cyclin-dependent kinase 7: at the cross-roads of transcription, DNA repair and cell cycle control?
1996,
Pubmed
O'Donovan,
Isolation of active recombinant XPG protein, a human DNA repair endonuclease.
1994,
Pubmed
O'Keefe,
Dynamic organization of DNA replication in mammalian cell nuclei: spatially and temporally defined replication of chromosome-specific alpha-satellite DNA sequences.
1992,
Pubmed
Raska,
Association between the nucleolus and the coiled body.
1990,
Pubmed
Raska,
Immunological and ultrastructural studies of the nuclear coiled body with autoimmune antibodies.
1991,
Pubmed
Reardon,
Isolation and characterization of two human transcription factor IIH (TFIIH)-related complexes: ERCC2/CAK and TFIIH.
1996,
Pubmed
Rebelo,
The dynamics of coiled bodies in the nucleus of adenovirus-infected cells.
1996,
Pubmed
Roy,
The MO15 cell cycle kinase is associated with the TFIIH transcription-DNA repair factor.
1994,
Pubmed
Sancar,
DNA excision repair.
1996,
Pubmed
Shiekhattar,
Cdk-activating kinase complex is a component of human transcription factor TFIIH.
1995,
Pubmed
Spector,
Macromolecular domains within the cell nucleus.
1993,
Pubmed
Tassan,
In vitro assembly of a functional human CDK7-cyclin H complex requires MAT1, a novel 36 kDa RING finger protein.
1995,
Pubmed
Tassan,
Cell cycle analysis of the activity, subcellular localization, and subunit composition of human CAK (CDK-activating kinase).
1994,
Pubmed
,
Echinobase
Trinczek,
In situ localization of cAMP-dependent protein kinases in nuclear and chromosomal substructures: relation to transcriptional activity.
1993,
Pubmed
Tuma,
Identification and characterization of a sphere organelle protein.
1993,
Pubmed
Wang,
The yeast TFB1 and SSL1 genes, which encode subunits of transcription factor IIH, are required for nucleotide excision repair and RNA polymerase II transcription.
1995,
Pubmed
Wu,
Snurposomes and coiled bodies.
1993,
Pubmed
Zawel,
Common themes in assembly and function of eukaryotic transcription complexes.
1995,
Pubmed