ECB-ART-36685
FEBS Lett
1997 Jun 30;4102-3:499-501. doi: 10.1016/s0014-5793(97)00641-8.
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Centrifugation does not alter spatial distribution of ''BEP4'' mRNA in paracentrotus lividus EGG.
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Paracentrotus lividus unfertilized eggs were centrifuged in a sucrose gradient, so to split each into two parts: a nucleated light fragment and an anucleated heavy fragment. Northern blot analyses utilizing a bep4 probe as animal marker and H2A histone gene and 12S-mit RNA as controls indicate that the eggs are elongated along the animal-vegetal axis during centrifugation and thereafter split into an animal and a vegetal half. Treatment of the eggs with colchicine before centrifugation abolishes the animal localization of bep4 mRNA.
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Genes referenced: h2a