Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Echinobase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Echinobase
ECB-ART-36668
Exp Cell Res 1997 Jul 10;2341:115-24. doi: 10.1006/excr.1997.3597.
Show Gene links Show Anatomy links

Time-resolved imaging of protein kinase C activation during sea urchin egg fertilization.

de Barry J , Kawahara S , Takamura K , Janoshazi A , Kirino Y , Olds JL , Lester DS , Alkon DL , Yoshioka T .


???displayArticle.abstract???
To study protein kinase C (PKC) activation during sea urchin egg fertilization we used three different fluorescent probes specific for PKC, namely, fim-1, which recognizes the catalytic site of the enzyme, and BODIPY- and NBD-phorbol esters interacting with the PKC regulatory domain. We were able to follow PKC activation during the early steps of fertilization, the three different probes giving the same fluorescent pattern. Within 120 s following insemination, the fluorescent signal increased and clustered in the cortical zone of the cell. The process was Ca2+ dependent and was inhibited in the presence of staurosporine, a PKC inhibitor. According to our in vitro probe characterization, this signal increase is due to PKC activation. These findings were further confirmed by Western blot analysis. This initial phase was followed by a rapid decrease which might be attributed to PKC hydrolysis by Ca2(+)-dependent proteases. The kinetics and the site distribution of PKC activation appear in complete agreement with the putative functions previously suggested for PKC during fertilization.

???displayArticle.pubmedLink??? 9223376
???displayArticle.link??? Exp Cell Res


Genes referenced: LOC100887844 LOC115919910 LOC586799 pkcl2