Adv Exp Med Biol 1997 Jan 01;419:431-6.

Ca(2+)-signalling in human T-lymphocytes. Potential roles for cyclic ADP-ribose and 2''-phospho-cyclic ADP-ribose.

???displayArticle.abstract???
Intracellular Ca(2+)-signals belong to the major events transducing extracellular signals into living cells. The discovery of (i) a caffeine-sensitive intracellular Ca(2+)-pool in Jurkat T-lymphocytes [1] and (ii) cyclic adenosine diphosphoribose (cADPR) as an agent that mobilizes Ca2+ from a caffeine- and ryanodine sensitive Ca(2+)-store in sea urchin egg homogenates [2] prompted us to investigate the potential role of this compound in T-lymphocyte Ca(2+)-signalling. cADPR, as well as its 2''-phosphorylated derivative, 2''-phospho-cADPR (2''-cADPR), released Ca2+ in a dose-dependent, specific manner from intracellular, non-endoplasmic reticular stores of permeabilized Jurkat and HPB. ALL T cells. In addition, attempts were made to prove the presence of endogenous cADPR and 2''-P-cADPR by HPLC. Several HPLC protocols, including microbore-HPLC were tested resulting in the detection of endogenous cADPR by sequential separation on strong-anion exchange HPLC and reverse-phase ion-pair HPLC.

???displayArticle.pubmedLink??? 9193685



Genes referenced: LOC100887844 LOC115919080