Zoolog Sci 1996 Dec 01;136:833-41. doi: 10.2108/zsj.13.833.

cDNA cloning of Na+, K(+)-ATPase alpha-subunit from embryos of the sea urchin, Hemicentrotus pulcherrimus.

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Na+, K(+)-ATPase alpha-subunit cDNA of the sea urchin, Hemicentrotus pulcherrimus, was obtained by twice screening prism and gastrula lambda gt10 cDNA libraries using an oligonucleotide probe derived from a mostly conserved region, FSBA (5''-p-(fluorosulfonyl)-benzoyladenosine) binding site of cation transport ATPases. The 5''-end of the non-coding region was determined by primer extension and the region was amplified by 5''-RACE method. The sea urchin alpha-subunit cDNA consists of 4401 nucleotides and encodes 1038 amino acid residues (MW, 114 kDa). The predicted primary structure, except N-terminal region, has similar degree of high homology to various metazoan Na+, K(+)-ATPase alpha-subunits. Alignment of amino acid sequence and a hydropathy profile also predicts eight putative transmembrane segments at least. The phylogenetic tree suspected from alignment of amino acid sequences of 21 species suggests that sea urchin and vertebrate Na+, K(+)-ATPase alpha-subunits seem to have evolved from a common origin, before vertebrate alpha-subunit divided into three isoforms.

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Genes referenced: LOC100887844 LOC581395