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We have identified an integral membrane protein of sea urchin gametes with an apparent molecular mass of 56 kD that cross-reacts with an antibody against the nucleoplasmic NH2-terminal domain of human lamin B receptor (LBR). In mature sperm, p56 is located at the tip and base of the nucleus from where it is removed by egg cytosol in vitro. In the egg, p56 is present in a subset of cytoplasmic membranes (MV2 beta) which contributes the bulk of the nuclear envelope during male pronuclear formation. p56-containing vesicles are required for nuclear envelope assembly and have a chromatin-binding capacity that is mediated by p56. Lamin B is not present in these vesicles and is imported into the nucleus from a soluble pool at a later stage of pronuclear formation. Lamin B incorporation and addition of new membranes are necessary for pronuclear swelling and nuclear envelope growth. We suggest that p56 is a sea urchin LBR homologue that targets membranes to chromatin and later anchors the membrane to the lamina.
Aebi,
The nuclear lamina is a meshwork of intermediate-type filaments.
1986, Pubmed
Aebi,
The nuclear lamina is a meshwork of intermediate-type filaments.
1986,
Pubmed
Appelbaum,
In vivo phosphorylation of the lamin B receptor. Binding of lamin B to its nuclear membrane receptor is affected by phosphorylation.
1990,
Pubmed
Bailer,
Characterization of A 54-kD protein of the inner nuclear membrane: evidence for cell cycle-dependent interaction with the nuclear lamina.
1991,
Pubmed
Burke,
A cell free system to study reassembly of the nuclear envelope at the end of mitosis.
1986,
Pubmed
Cameron,
In vitro development of the sea urchin male pronucleus.
1994,
Pubmed
,
Echinobase
Chaudhary,
Stepwise reassembly of the nuclear envelope at the end of mitosis.
1993,
Pubmed
Collas,
Distinct egg membrane vesicles differing in binding and fusion properties contribute to sea urchin male pronuclear envelopes formed in vitro.
1996,
Pubmed
,
Echinobase
Collas,
Lamin dynamics during sea urchin male pronuclear formation in vitro.
1995,
Pubmed
,
Echinobase
Collas,
Lipophilic organizing structures of sperm nuclei target membrane vesicle binding and are incorporated into the nuclear envelope.
1995,
Pubmed
,
Echinobase
Courvalin,
The lamin B receptor of the inner nuclear membrane undergoes mitosis-specific phosphorylation and is a substrate for p34cdc2-type protein kinase.
1992,
Pubmed
Courvalin,
Identification and characterization of autoantibodies against the nuclear envelope lamin B receptor from patients with primary biliary cirrhosis.
1990,
Pubmed
Dabauvalle,
Inhibition of nuclear accumulation of karyophilic proteins in living cells by microinjection of the lectin wheat germ agglutinin.
1988,
Pubmed
Dwyer,
A modified procedure for the isolation of a pore complex-lamina fraction from rat liver nuclei.
1976,
Pubmed
Finlay,
Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores.
1987,
Pubmed
Fisher,
cDNA sequencing of nuclear lamins A and C reveals primary and secondary structural homology to intermediate filament proteins.
1986,
Pubmed
Foisner,
Integral membrane proteins of the nuclear envelope interact with lamins and chromosomes, and binding is modulated by mitotic phosphorylation.
1993,
Pubmed
Furukawa,
Cloning of a cDNA for lamina-associated polypeptide 2 (LAP2) and identification of regions that specify targeting to the nuclear envelope.
1995,
Pubmed
Georgatos,
Lamins and lamin-associated proteins.
1994,
Pubmed
Gerace,
Integral membrane proteins and dynamic organization of the nuclear envelope.
1994,
Pubmed
Gerace,
The nuclear envelope lamina is reversibly depolymerized during mitosis.
1980,
Pubmed
Glass,
Lamins A and C bind and assemble at the surface of mitotic chromosomes.
1990,
Pubmed
Harris,
Three distinct human thymopoietins are derived from alternatively spliced mRNAs.
1994,
Pubmed
Hinshaw,
Architecture and design of the nuclear pore complex.
1992,
Pubmed
Holy,
Molecular characterization and expression patterns of a B-type nuclear lamin during sea urchin embryogenesis.
1995,
Pubmed
,
Echinobase
Longo,
The fine structure of pronuclear development and fusion in the sea urchin, Arbacia punctulata.
1968,
Pubmed
,
Echinobase
Lourim,
Membrane-associated lamins in Xenopus egg extracts: identification of two vesicle populations.
1993,
Pubmed
Lourim,
Lamin-dependent nuclear envelope reassembly following mitosis: an argument.
1994,
Pubmed
Martin,
cDNA cloning and characterization of lamina-associated polypeptide 1C (LAP1C), an integral protein of the inner nuclear membrane.
1995,
Pubmed
Matsuura,
Distribution and induction of cytochrome P-450 in rat liver nuclear envelope.
1981,
Pubmed
McKeon,
Homologies in both primary and secondary structure between nuclear envelope and intermediate filament proteins.
1986,
Pubmed
Meier,
Type B lamins remain associated with the integral nuclear envelope protein p58 during mitosis: implications for nuclear reassembly.
1994,
Pubmed
Meier,
The role of lamin LIII in nuclear assembly and DNA replication, in cell-free extracts of Xenopus eggs.
1991,
Pubmed
Newport,
A lamin-independent pathway for nuclear envelope assembly.
1990,
Pubmed
Nikolakaki,
A nuclear envelope-associated kinase phosphorylates arginine-serine motifs and modulates interactions between the lamin B receptor and other nuclear proteins.
1996,
Pubmed
Poccia,
Nuclei and chromosomal proteins.
1986,
Pubmed
,
Echinobase
Schuler,
Characterization of the human gene encoding LBR, an integral protein of the nuclear envelope inner membrane.
1994,
Pubmed
Sheehan,
Steps in the assembly of replication-competent nuclei in a cell-free system from Xenopus eggs.
1988,
Pubmed
Simos,
The inner nuclear membrane protein p58 associates in vivo with a p58 kinase and the nuclear lamins.
1992,
Pubmed
Smith,
Colocalization of vertebrate lamin B and lamin B receptor (LBR) in nuclear envelopes and in LBR-induced membrane stacks of the yeast Saccharomyces cerevisiae.
1994,
Pubmed
Soullam,
Signals and structural features involved in integral membrane protein targeting to the inner nuclear membrane.
1995,
Pubmed
Soullam,
The amino-terminal domain of the lamin B receptor is a nuclear envelope targeting signal.
1993,
Pubmed
Ulitzur,
Lamin activity is essential for nuclear envelope assembly in a Drosophila embryo cell-free extract.
1992,
Pubmed
Vigers,
A distinct vesicle population targets membranes and pore complexes to the nuclear envelope in Xenopus eggs.
1991,
Pubmed
Wilson,
A trypsin-sensitive receptor on membrane vesicles is required for nuclear envelope formation in vitro.
1988,
Pubmed
Worman,
A lamin B receptor in the nuclear envelope.
1988,
Pubmed
Ye,
Interaction between an integral protein of the nuclear envelope inner membrane and human chromodomain proteins homologous to Drosophila HP1.
1996,
Pubmed
Ye,
Primary structure analysis and lamin B and DNA binding of human LBR, an integral protein of the nuclear envelope inner membrane.
1994,
Pubmed
