Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Glycoconj J
1996 Jun 01;133:401-13. doi: 10.1007/bf00731473.
Show Gene links
Show Anatomy links
Isolation and identification of novel sulfated and nonsulfated oligosialyl glycosphingolipids from sea urchin sperm.
Ijuin T
,
Kitajima K
,
Song Y
,
Kitazume S
,
Inoue S
,
Haslam SM
,
Morris HR
,
Dell A
,
Inoue Y
.
???displayArticle.abstract???
Novel sulfated and nonsulfated oligosialylglycosphingolipids were isolated from sperm of the sea urchin, Hemicentrotus pulcherrimus, and their structures were established as follows: [formula: see text] This provides the first evidence for the natural occurrence of a tetrasialic acid structure in glycosphingolipids. The finding of sulfated oligosialyl chains is especially noteworthy in that the sulfate group exclusively resides on the C-8 of the nonreducing terminal residues of oligo/polysialyl chains and that sulfation appears to be a termination signal for elongation of oligosialyl chains. Sulfation at the nonreducing terminal Neu5Ac residues of oligosialyl chains was also found to facilitate the formation of an inter-residue lactone between the carboxyl group at the nonreducing terminal sulfated Neu5Ac and the hydroxyl group at C-9 of the penultimate Neu5Ac residue. The long chain base was 4-hydroxysphinganine (t18:0) and the major fatty acid species were identified as C20:1, C21:1, and C22:1.
Ando,
High resolution proton NMR studies of gangliosides. Structure of two types of GD3 lactones and their reactivity with monoclonal antibody R24.
1989, Pubmed
Ando,
High resolution proton NMR studies of gangliosides. Structure of two types of GD3 lactones and their reactivity with monoclonal antibody R24.
1989,
Pubmed
Ando,
Isolation and characterization of two isomers of brain tetrasialogangliosides.
1979,
Pubmed
Angata,
Identification, characterization, and developmental expression of a novel alpha 2-->8-KDN-transferase which terminates elongation of alpha 2-->8-linked oligo-polysialic acid chain synthesis in trout egg polysialoglycoproteins.
1994,
Pubmed
Anumula,
A comprehensive procedure for preparation of partially methylated alditol acetates from glycoprotein carbohydrates.
1992,
Pubmed
Dell,
Mass spectrometry of carbohydrate-containing biopolymers.
1994,
Pubmed
FOLCH,
A simple method for the isolation and purification of total lipides from animal tissues.
1957,
Pubmed
Hoshi,
Novel sialosphingolipids from spermatozoa of the sea urchin Anthocidaris crassispina.
1975,
Pubmed
,
Echinobase
Inoue,
Stability of N-acyl groups in methylated alpha 2 leads to 8-linked oligosialosyl chains toward methanolysis. Analysis by chemical ionization-mass spectrometry.
1982,
Pubmed
Ishizuka,
An isomer of trisialoganglioside and the sturcture of tetra- and pentasialogangliosides from fish brain.
1972,
Pubmed
Iwasaki,
Isolation and structures of the third major type of carbohydrate units in polysialoglycoproteins from rainbow trout eggs.
1984,
Pubmed
Kitazume,
Identification of polysialic acid-containing glycoprotein in the jelly coat of sea urchin eggs. Occurrence of a novel type of polysialic acid structure.
1994,
Pubmed
,
Echinobase
Kochetkov,
Isolation and structural studies of a sulfated sialosphingolipid from the sea urchin Echinocardium cordatum.
1976,
Pubmed
,
Echinobase
Koerner,
High-resolution proton NMR studies of gangliosides. 1. Use of homonuclear two-dimensional spin-echo J-correlated spectroscopy for determination of residue composition and anomeric configurations.
1983,
Pubmed
Nadano,
A naturally occurring deaminated neuraminic acid, 3-deoxy-D-glycero-D-galacto-nonulosonic acid (KDN). Its unique occurrence at the nonreducing ends of oligosialyl chains in polysialoglycoprotein of rainbow trout eggs.
1986,
Pubmed
Nomoto,
Structures of carbohydrate units isolated from trout egg polysialoglycoproteins: short-cored units with oligosialosyl groups.
1982,
Pubmed
Prokazova,
Unusual gangliosides of eggs and embryos of the sea urchin Strongylocentrotus intermedius. Structure and density-dependence of surface localization.
1981,
Pubmed
,
Echinobase
Song,
Isolation and structural elucidation of a novel type of ganglioside, deaminated neuraminic acid (KDN)-containing glycosphingolipid, from rainbow trout sperm. The first example of the natural occurrence of KDN-ganglioside, (KDN)GM3.
1991,
Pubmed
Song,
Expression of new KDN-gangliosides in rainbow trout testis during spermatogenesis and their structural identification.
1995,
Pubmed
Song,
Structure of novel gangliosides, deaminated neuraminic acid (KDN)-containing glycosphingolipids, isolated from rainbow trout ovarian fluid.
1993,
Pubmed
SVENNERHOLM,
Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method.
1957,
Pubmed
Tanphaichitr,
Role of a gamete-specific sulfoglycolipid immobilizing protein on mouse sperm-egg binding.
1993,
Pubmed
Yu,
Gas--liquid chromatographic assay of lipid-bound sialic acids: measurement of gangliosides in brain of several species.
1970,
Pubmed
