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Single amino acid mutations in Drosophila fascin disrupt actin bundling function in vivo.
Cant K
,
Cooley L
.
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Fascins bundle actin filaments into large, tightly packed hexagonal arrays that support diverse cellular processes including microvillar projections and filopodial extensions. In Drosophila, fascin is encoded by the singed locus. Severe singed mutants have gnarled bristles and are female sterile due to a defect in rapid cytoplasm transport during oogenesis. In this paper, we report the results of a large EMS mutagenesis screen to generate new singed alleles. A mutation that changes glycine 409 to glutamic acid results in partial inactivation of fascin in vivo; singedG409E mutants have kinked bristles and are fertile with a mild nurse cell cytoplasm transport defect. This mutation is in a small conserved domain near the C-terminus of fascin. A mutation that changes serine 289 to asparagine almost completely inactivates fascin in vivo; singedS289N mutants have gnarled bristles and are sterile due to a severe defect in nurse cell cytoplasm transport caused by the absence of nurse cell cytoplasmic actin bundles. A subsequent EMS mutagenesis screen for dominant suppressors of singedS289N sterility revealed an intragenic suppressor mutation that changes serine 251 to phenylalanine and restores much of fascin''s function. These two mutations, S289N and S251F, draw attention to a central domain in fascin.
Adams,
Formation of stable microspikes containing actin and the 55 kDa actin bundling protein, fascin, is a consequence of cell adhesion to thrombospondin-1: implications for the anti-adhesive activities of thrombospondin-1.
1995, Pubmed
Adams,
Formation of stable microspikes containing actin and the 55 kDa actin bundling protein, fascin, is a consequence of cell adhesion to thrombospondin-1: implications for the anti-adhesive activities of thrombospondin-1.
1995,
Pubmed
Adams,
A yeast actin-binding protein is encoded by SAC6, a gene found by suppression of an actin mutation.
1989,
Pubmed
Adams,
Requirement of yeast fimbrin for actin organization and morphogenesis in vivo.
1991,
Pubmed
Adams,
Dominant suppressors of yeast actin mutations that are reciprocally suppressed.
1989,
Pubmed
Appel,
The Drosophila Stubble-stubbloid gene encodes an apparent transmembrane serine protease required for epithelial morphogenesis.
1993,
Pubmed
Bender,
Studies on the Expression of Various Singed Alleles in Drosophila Melanogaster.
1960,
Pubmed
Bryan,
Separation and interaction of the major components of sea urchin actin gel.
1978,
Pubmed
,
Echinobase
Bryan,
Fascin, an echinoid actin-bundling protein, is a homolog of the Drosophila singed gene product.
1993,
Pubmed
,
Echinobase
Cant,
Drosophila singed, a fascin homolog, is required for actin bundle formation during oogenesis and bristle extension.
1994,
Pubmed
,
Echinobase
Church,
Genomic sequencing.
1984,
Pubmed
Cooley,
chickadee encodes a profilin required for intercellular cytoplasm transport during Drosophila oogenesis.
1992,
Pubmed
DeRosier,
Structure of F-actin needles from extracts of sea urchin oocytes.
1981,
Pubmed
,
Echinobase
Drubin,
Yeast actin-binding proteins: evidence for a role in morphogenesis.
1988,
Pubmed
Edwards,
Fascins, a family of actin bundling proteins.
1995,
Pubmed
,
Echinobase
Edwards,
Cloning and expression of a murine fascin homolog from mouse brain.
1995,
Pubmed
Gans,
Isolation and characterization of sex-linked female-sterile mutants in Drosophila melanogaster.
1975,
Pubmed
Gutzeit,
The role of microfilaments in cytoplasmic streaming in Drosophila follicles.
1986,
Pubmed
Heberlein,
The C-terminus of the homeodomain is required for functional specificity of the Drosophila rough gene.
1994,
Pubmed
Holthuis,
A vertebrate homolog of the actin-bundling protein fascin.
1994,
Pubmed
,
Echinobase
Honts,
Actin mutations that show suppression with fimbrin mutations identify a likely fimbrin-binding site on actin.
1994,
Pubmed
Hoover,
Effects of transposable elements on the expression of the forked gene of Drosophila melanogaster.
1993,
Pubmed
Kane,
Actin polymerization and interaction with other proteins in temperature-induced gelation of sea urchin egg extracts.
1976,
Pubmed
,
Echinobase
Komitopoulou,
Isolation and Characterization of Sex-Linked Female-Sterile Mutants in DROSOPHILA MELANOGASTER with Special Attention to Eggshell Mutants.
1983,
Pubmed
Mahajan-Miklos,
The villin-like protein encoded by the Drosophila quail gene is required for actin bundle assembly during oogenesis.
1994,
Pubmed
Mosialos,
Epstein-Barr virus infection induces expression in B lymphocytes of a novel gene encoding an evolutionarily conserved 55-kilodalton actin-bundling protein.
1994,
Pubmed
,
Echinobase
Otto,
Formation of filopodia in coelomocytes: localization of fascin, a 58,000 dalton actin cross-linking protein.
1979,
Pubmed
,
Echinobase
Otto,
Redistribution of actin and fascin in sea urchin eggs after fertilization.
1980,
Pubmed
,
Echinobase
Overton,
The fine structure of developing bristles in wild type and mutant Drosophila melanogaster.
1967,
Pubmed
Paterson,
Structure and transcription of the singed locus of Drosophila melanogaster.
1991,
Pubmed
Petersen,
forked proteins are components of fiber bundles present in developing bristles of Drosophila melanogaster.
1994,
Pubmed
Qian,
Structural analysis of the Drosophila rpA1 gene, a member of the eucaryotic 'A' type ribosomal protein family.
1987,
Pubmed
Spudich,
Structure of actin filament bundles from microvilli of sea urchin eggs.
1979,
Pubmed
,
Echinobase
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Wheatley,
Drosophila nonmuscle myosin II is required for rapid cytoplasmic transport during oogenesis and for axial nuclear migration in early embryos.
1995,
Pubmed
Xue,
kelch encodes a component of intercellular bridges in Drosophila egg chambers.
1993,
Pubmed
Yamashiro-Matsumura,
Purification and characterization of an F-actin-bundling 55-kilodalton protein from HeLa cells.
1985,
Pubmed
Yamashiro-Matsumura,
Intracellular localization of the 55-kD actin-bundling protein in cultured cells: spatial relationships with actin, alpha-actinin, tropomyosin, and fimbrin.
1986,
Pubmed
