Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Functional domains of chlamydial histone H1-like protein.
Remacha M
,
Kaul R
,
Sherburne R
,
Wenman WM
.
???displayArticle.abstract???
Chlamydial trachomatis is one of the few prokaryotic organisms known to contain proteins that bear amino acid similarity to eukaryotic histone H1. It is also appreciated that chlamydial histone-like proteins, designated Hc1 and Hc2, can bind DNA and are presumably involved in the condensation of infectious elementary bodies. However, there is no information on either the orientation of Hc1 and Hc2 or the mechanism of their DNA-protein and protein-protein interactions. Whereas the C-terminal domain of Hc1 between amino acids 63 and 125 shows best alignment with sea-urchin histone H1, and N-terminus between amino acids 1 and 62 is highly conserved among various chlamydial species, suggesting a bifunctional role for this unique protein. In order to delineate the regions responsible for the Hc1 characteristics, we have expressed these two fragments independently in Escherichia coli and studied the binding of double-stranded DNA to either whole Hc1 protein or its two termini. Our results support the role of the carboxyl portion in DNA-protein interaction, a function similar to its eukaryotic counterpart. Although this interaction initiates DNA condensation in the absence of the N-terminal domain, it is not sufficient to produce complete compaction. Intra- or inter-molecular protein-protein interactions may be necessary to achieve such an effect.
Allan,
The structure of histone H1 and its location in chromatin.
1980, Pubmed
Allan,
The structure of histone H1 and its location in chromatin.
1980,
Pubmed
Armbruster,
Specimen preparation for electron microscopy using low temperature embedding resins.
1982,
Pubmed
Barry,
Nucleoid condensation in Escherichia coli that express a chlamydial histone homolog.
1992,
Pubmed
Barry,
Hc1-mediated effects on DNA structure: a potential regulator of chlamydial development.
1993,
Pubmed
Birnboim,
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
1979,
Pubmed
Brickman,
Molecular cloning and expression of hctB encoding a strain-variant chlamydial histone-like protein with DNA-binding activity.
1993,
Pubmed
Christiansen,
Interaction between the Chlamydia trachomatis histone H1-like protein (Hc1) and DNA.
1993,
Pubmed
Cole,
Microheterogeneity in H1 histones and its consequences.
1987,
Pubmed
Comanducci,
The structure of a plasmid of Chlamydia trachomatis believed to be required for growth within mammalian cells.
1988,
Pubmed
Costerton,
Ultrastructural studies of the nucleoids of the pleomorphic forms of Chlamydia psittaci 6BC: a comparison with bacteria.
1976,
Pubmed
Eick,
Human H1 histones: conserved and varied sequence elements in two H1 subtype genes.
1989,
Pubmed
Feinberg,
"A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.
1984,
Pubmed
Hackstadt,
Chlamydia trachomatis developmentally regulated protein is homologous to eukaryotic histone H1.
1991,
Pubmed
Hackstadt,
Diversity in the Chlamydia trachomatis histone homologue Hc2.
1993,
Pubmed
Hanahan,
Studies on transformation of Escherichia coli with plasmids.
1983,
Pubmed
Holmes,
A rapid boiling method for the preparation of bacterial plasmids.
1981,
Pubmed
Kaul,
Expression of the Chlamydia trachomatis major outer membrane protein-encoding gene in Escherichia coli: role of the 3' end in mRNA stability.
1990,
Pubmed
Kaul,
Interspecies structural diversity among chlamydial genes encoding histone H1.
1992,
Pubmed
,
Echinobase
Kunkel,
Rapid and efficient site-specific mutagenesis without phenotypic selection.
1987,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Moulder,
Interaction of chlamydiae and host cells in vitro.
1991,
Pubmed
Olmsted,
Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples.
1981,
Pubmed
Pedersen,
Interaction of the Chlamydia trachomatis histone H1-like protein (Hc1) with DNA and RNA causes repression of transcription and translation in vitro.
1994,
Pubmed
Perara,
A developmentally regulated chlamydial gene with apparent homology to eukaryotic histone H1.
1992,
Pubmed
,
Echinobase
Roth,
Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue.
1981,
Pubmed
Short,
Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties.
1988,
Pubmed
Studier,
Use of T7 RNA polymerase to direct expression of cloned genes.
1990,
Pubmed
Tao,
Identification and nucleotide sequence of a developmentally regulated gene encoding a eukaryotic histone H1-like protein from Chlamydia trachomatis.
1991,
Pubmed
,
Echinobase
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Wagar,
Developmental-form-specific DNA-binding proteins in Chlamydia spp.
1988,
Pubmed
Wenman,
Chlamydia trachomatis elementary bodies possess proteins which bind to eucaryotic cell membranes.
1986,
Pubmed