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We have identified a clear preference of histone H1 for CpG-methylated DNA, irrespective of DNA sequence. The conditions under which this preference is observed allowed cooperative binding of H1; the H1-DNA complexes formed were shown earlier to be ''tramlines'' of two DNA duplexes bridged by an array of H1 molecules, and multiples of these. The preference for methylated DNA is clear in sedimentation assays, which also show that the preference is greater with increased methylation level, and in gel retardation assays with an oligonucleotide containing a single methyl-CpG pair; it is shared by the globular domain which also binds cooperatively to DNA. A small intrinsic preference of H1 for methylated DNA is also apparent in Southwestern assays where the immobilized H1 presumably cannot bind cooperatively. Methylated DNA in H1-DNA complexes was partially protected (relative to unmethylated DNA) against digestion by MspI but not by enzymes whose cutting sites were not methylated, consistent with a direct interaction of H1 with methylated nucleotides; this was also true of GH1-DNA complexes. H1 variants (spH1 and H5) from transcriptionally repressed nuclei have a stronger preference than H1 for methylated DNA, suggesting that this may be relevant to the stabilization of chromatin higher order structure and transcriptional repression.
Ball,
5-methylcytosine is localized in nucleosomes that contain histone H1.
1983, Pubmed
Ball,
5-methylcytosine is localized in nucleosomes that contain histone H1.
1983,
Pubmed
Bates,
Stability of the higher-order structure of chicken-erythrocyte chromatin in solution.
1981,
Pubmed
Bird,
CpG-rich islands and the function of DNA methylation.
1986,
Pubmed
Bird,
Methylated and unmethylated DNA compartments in the sea urchin genome.
1979,
Pubmed
,
Echinobase
Buschhausen,
Chromatin structure is required to block transcription of the methylated herpes simplex virus thymidine kinase gene.
1987,
Pubmed
Cedar,
DNA methylation and gene activity.
1988,
Pubmed
Cerf,
Homo- and heteronuclear two-dimensional NMR studies of the globular domain of histone H1: full assignment, tertiary structure, and comparison with the globular domain of histone H5.
1994,
Pubmed
Clark,
Differences in the binding of H1 variants to DNA. Cooperativity and linker-length related distribution.
1988,
Pubmed
,
Echinobase
Clark,
Salt-dependent co-operative interaction of histone H1 with linear DNA.
1986,
Pubmed
Clark,
Alpha-helix in the carboxy-terminal domains of histones H1 and H5.
1988,
Pubmed
,
Echinobase
Draves,
Co-operative binding of the globular domain of histone H5 to DNA.
1992,
Pubmed
Hayes,
Contacts of the globular domain of histone H5 and core histones with DNA in a "chromatosome".
1994,
Pubmed
Higurashi,
The combination of DNA methylation and H1 histone binding inhibits the action of a restriction nuclease on plasmid DNA.
1991,
Pubmed
Hill,
Histone-DNA interactions and their modulation by phosphorylation of -Ser-Pro-X-Lys/Arg- motifs.
1991,
Pubmed
,
Echinobase
Johnson,
The effect of histone H1 and DNA methylation on transcription.
1995,
Pubmed
Jost,
The repressor MDBP-2 is a member of the histone H1 family that binds preferentially in vitro and in vivo to methylated nonspecific DNA sequences.
1992,
Pubmed
Kamakaka,
Chromatin structure of transcriptionally competent and repressed genes.
1990,
Pubmed
Keshet,
DNA methylation affects the formation of active chromatin.
1986,
Pubmed
Kim,
Bending of DNA by gene-regulatory proteins: construction and use of a DNA bending vector.
1989,
Pubmed
Levine,
Histone H1-mediated inhibition of transcription initiation of methylated templates in vitro.
1993,
Pubmed
Lewis,
Purification, sequence, and cellular localization of a novel chromosomal protein that binds to methylated DNA.
1992,
Pubmed
Meehan,
Characterization of MeCP2, a vertebrate DNA binding protein with affinity for methylated DNA.
1992,
Pubmed
Meehan,
Identification of a mammalian protein that binds specifically to DNA containing methylated CpGs.
1989,
Pubmed
Miskimins,
Use of a protein-blotting procedure and a specific DNA probe to identify nuclear proteins that recognize the promoter region of the transferrin receptor gene.
1985,
Pubmed
Nightingale,
Methylation at CpG sequences does not influence histone H1 binding to a nucleosome including a Xenopus borealis 5 S rRNA gene.
1995,
Pubmed
Noll,
Preparation of native chromatin and damage caused by shearing.
1975,
Pubmed
Ramakrishnan,
Crystal structure of globular domain of histone H5 and its implications for nucleosome binding.
1993,
Pubmed
Renz,
Preferential and cooperative binding of histone I to chromosomal mammalian DNA.
1975,
Pubmed
Supakar,
Methylated DNA-binding protein is present in various mammalian cell types.
1988,
Pubmed
Tazi,
Alternative chromatin structure at CpG islands.
1990,
Pubmed
Thoma,
Involvement of histone H1 in the organization of the nucleosome and of the salt-dependent superstructures of chromatin.
1979,
Pubmed
Thomas,
The study of histone--histone associations by chemical cross-linking.
1978,
Pubmed
Thomas,
Salt-induced folding of sea urchin sperm chromatin.
1986,
Pubmed
,
Echinobase
Thomas,
Cooperative binding of the globular domains of histones H1 and H5 to DNA.
1992,
Pubmed
Vinson,
In situ detection of sequence-specific DNA binding activity specified by a recombinant bacteriophage.
1988,
Pubmed
Waalwijk,
MspI, an isoschizomer of hpaII which cleaves both unmethylated and methylated hpaII sites.
1978,
Pubmed
