Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Virchows Arch
1995 Jan 01;4274:401-6. doi: 10.1007/bf00199389.
Show Gene links
Show Anatomy links
Immunohistochemical analysis of rat and human respiratory cilia with anti-dynein antibody: comparison between normal cilia and pathological cilia in primary ciliary dyskinesia.
Umeda A
,
Yoshida T
,
Yamaguchi K
,
Kanazawa M
,
Torikata C
.
???displayArticle.abstract???
Wistar Imamichi rat and human respiratory cilia were examined with anti-dynein antibody (AD2), which is specific for sea urchin sperm flagellar dynein. AD2-labelled fresh-frozen normal rat and human cilia stained clearly by immunofluorescence and the peroxidase-antiperoxidase (PAP) technique. On immunoelectron microscopy, AD2 labelled the outer dynein arms of normal human cilia. Paraffin-embedded normal human cilia also stained by immunofluorescence, although not always clearly. Neither the cilia of WIC-Hyd male rats, an animal model of Kartagener''s syndrome, nor human cilia from patients with primary ciliary dyskinesia (PCD) reacted positively by the immunofluorescence or PAP technique. Western blots of normal rat cilia yielded a single band of about 450 kDa. In conclusion, AD2 recognizes the outer arm dynein heavy chains of healthy cilia and may be useful in diagnosing and classifying PCD light microscopically especially when only paraffin-embedded specimens are available. This approach may be of potential use for better defining and classifying PCD.
Afzelius,
A human syndrome caused by immotile cilia.
1976, Pubmed
Afzelius,
A human syndrome caused by immotile cilia.
1976,
Pubmed
Brokaw,
Bending patterns of Chlamydomonas flagella: IV. Mutants with defects in inner and outer dynein arms indicate differences in dynein arm function.
1987,
Pubmed
Camner,
Evidence of congenitally nonfunctioning cilia in the tracheobronchial tract in two subjects.
1975,
Pubmed
Gibbons,
Dynein: A Protein with Adenosine Triphosphatase Activity from Cilia.
1965,
Pubmed
Koto,
Inherited hydrocephalus in Csk: Wistar-Imamichi rats; Hyd strain: a new disease model for hydrocephalus.
1987,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Nogawa,
[A case of immotile cilia syndrome and a review of Japanese cases].
1989,
Pubmed
Pollard,
Fixation, processing, and immunochemical reagent effects on preservation of T-lymphocyte surface membrane antigens in paraffin-embedded tissue.
1987,
Pubmed
Rott,
Kartagener's syndrome and the syndrome of immotile cilia.
1979,
Pubmed
Rutland,
Non-invasive sampling of nasal cilia for measurement of beat frequency and study of ultrastructure.
1980,
Pubmed
Sato,
The AMeX method. A simplified technique of tissue processing and paraffin embedding with improved preservation of antigens for immunostaining.
1986,
Pubmed
Sleigh,
Primary ciliary dyskinesia.
1981,
Pubmed
Torikata,
Nine Japanese patients with immotile-dyskinetic cilia syndrome: an ultrastructural study using tannic acid-containing fixation.
1991,
Pubmed
Torikata,
Ultrastructure of respiratory cilia of WIC-Hyd male rats. An animal model for human immotile cilia syndrome.
1991,
Pubmed
Witman,
Axonemal dyneins.
1992,
Pubmed
Yokota,
Ultrastructure and function of cilia and spermatozoa flagella in a patient with Kartagener's syndrome.
1993,
Pubmed
Yoshida,
Analysis of mammalian dynein using antibodies against A polypeptides of sea urchin sperm flagellar dynein.
1989,
Pubmed
,
Echinobase
