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Proc Natl Acad Sci U S A
1993 Feb 01;903:928-32. doi: 10.1073/pnas.90.3.928.
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Primary structure of two-chain botrocetin, a von Willebrand factor modulator purified from the venom of Bothrops jararaca.
Usami Y
,
Fujimura Y
,
Suzuki M
,
Ozeki Y
,
Nishio K
,
Fukui H
,
Titani K
.
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The complete amino acid sequence and location of the disulfide bonds of two-chain botrocetin, which promotes platelet agglutination in the presence of von Willebrand factor, from venom of the snake Bothrops jararaca are presented. Sequences of the alpha and beta subunits were determined by analysis of peptides generated by digestion of the S-pyridylethylated protein with Achromobacter protease I or alpha-chymotrypsin and by chemical cleavage with cyanogen bromide or 2-(2''-nitrophenylsulfenyl)-3-methyl-3-bromoindolenine. Two-chain botrocetin is a heterodimer composed of the alpha subunit (consisting of 133 amino acid residues) and the beta subunit (consisting of 125 amino acid residues) held together by a disulfide bond. Seven disulfide bonds link half-cystine residues 2 to 13, 30 to 128, and 103 to 120 of the alpha subunit; 2 to 13, 30 to 121, and 98 to 113 of the beta subunit; and 80 of the alpha subunit to 75 of the beta subunit. In terms of amino acid sequence and disulfide bond location, two-chain botrocetin is homologous to echinoidin (a sea urchin lectin) and other C-type (Ca(2+)-dependent) lectins.
Andrews,
Purification of botrocetin from Bothrops jararaca venom. Analysis of the botrocetin-mediated interaction between von Willebrand factor and the human platelet membrane glycoprotein Ib-IX complex.
1989, Pubmed
Andrews,
Purification of botrocetin from Bothrops jararaca venom. Analysis of the botrocetin-mediated interaction between von Willebrand factor and the human platelet membrane glycoprotein Ib-IX complex.
1989,
Pubmed
Brinkhous,
Botrocetin (venom coagglutinin): reaction with a broad spectrum of multimeric forms of factor VIII macromolecular complex.
1983,
Pubmed
De Marco,
Properties of human asialo-factor VIII. A ristocetin-independent platelet-aggregating agent.
1981,
Pubmed
Drickamer,
Two distinct classes of carbohydrate-recognition domains in animal lectins.
1988,
Pubmed
Fujimura,
Studies on anti-von Willebrand factor (vWF) monoclonal antibody NMC-4, which inhibits both ristocetin- and botrocetin-induced vWF binding to platelet glycoprotein Ib.
1991,
Pubmed
Fujimura,
The interaction of botrocetin with normal or variant von Willebrand factor (types IIA and IIB) and its inhibition by monoclonal antibodies that block receptor binding.
1992,
Pubmed
,
Echinobase
Fujimura,
Isolation and chemical characterization of two structurally and functionally distinct forms of botrocetin, the platelet coagglutinin isolated from the venom of Bothrops jararaca.
1991,
Pubmed
Fujimura,
The von willebrand factor domain-mediating botrocetin-induced binding to glycoprotein IB lies between Val449 and Lys728.
1987,
Pubmed
Giga,
The complete amino acid sequence of echinoidin, a lectin from the coelomic fluid of the sea urchin Anthocidaris crassispina. Homologies with mammalian and insect lectins.
1987,
Pubmed
,
Echinobase
Hermodson,
Determination of the amino acid sequence of porcine trypsin by sequenator aalysis.
1973,
Pubmed
Hirabayashi,
Complete primary structure of a galactose-specific lectin from the venom of the rattlesnake Crotalus atrox. Homologies with Ca2(+)-dependent-type lectins.
1991,
Pubmed
Howard,
Ristocetin--a new tool in the investigation of platelet aggregation.
1971,
Pubmed
Howard,
The agglutination of human platelets by botrocetin: evidence that botrocetin and ristocetin act at different sites on the factor VIII molecule and platelet membrane.
1984,
Pubmed
Knecht,
Liquid chromatographic determination of amino acids after gas-phase hydrolysis and derivatization with (dimethylamino)azobenzenesulfonyl chloride.
1986,
Pubmed
Krusius,
A fibroblast chondroitin sulfate proteoglycan core protein contains lectin-like and growth factor-like sequences.
1987,
Pubmed
Mahoney,
Separation of large denatured peptides by reverse phase high performance liquid chromatography. Trifluoroacetic acid as a peptide solvent.
1980,
Pubmed
Masaki,
Studies on a new proteolytic enzyme from A chromobacter lyticus M497-1. I. Purification and some enzymatic properties.
1981,
Pubmed
Mohri,
Structure of the von Willebrand factor domain interacting with glycoprotein Ib.
1988,
Pubmed
Nishio,
Enhanced botrocetin-induced type IIB von Willebrand factor binding to platelet glycoprotein Ib initiates hyperagglutination of normal platelets.
1990,
Pubmed
Omenn,
Modification of the single tryptophan residue of staphylococcal nuclease by a new mild oxidizing agent.
1970,
Pubmed
Read,
Venom coagglutinin: an activator of platelet aggregation dependent on von Willebrand factor.
1978,
Pubmed
Read,
Venom coagglutinin for detection of von Willebrand factor activity in animal plasmas.
1983,
Pubmed
Read,
Role of botrocetin in platelet agglutination: formation of an activated complex of botrocetin and von Willebrand factor.
1989,
Pubmed
Rüegg,
Reductive cleavage of cystine disulfides with tributylphosphine.
1977,
Pubmed
Sadler,
von Willebrand factor.
1991,
Pubmed
Spiess,
Sequence of a second human asialoglycoprotein receptor: conservation of two receptor genes during evolution.
1985,
Pubmed
Sugimoto,
Identification of discontinuous von Willebrand factor sequences involved in complex formation with botrocetin. A model for the regulation of von Willebrand factor binding to platelet glycoprotein Ib.
1991,
Pubmed
Weiss,
Von Willebrand factor: dissociation from antihemophilic factor procoagulant activity.
1973,
Pubmed
Wilbur,
Rapid similarity searches of nucleic acid and protein data banks.
1983,
Pubmed
