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Drosophila singed, a fascin homolog, is required for actin bundle formation during oogenesis and bristle extension.
Cant K
,
Knowles BA
,
Mooseker MS
,
Cooley L
.
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Drosophila singed mutants were named for their gnarled bristle phenotype but severe alleles are also female sterile. Recently, singed protein was shown to have 35% peptide identity with echinoderm fascin. Fascin is found in actin filament bundles in microvilli of sea urchin eggs and in filopodial extensions in coelomocytes. We show that Drosophila singed is required for actin filament bundle formation in the cytoplasm of nurse cells during oogenesis; in severe mutants, the absence of cytoplasmic actin filament bundles allows nurse cell nuclei to lodge in ring canals and block nurse cell cytoplasm transport. Singed is also required for organized actin filament bundle formation in the cellular extension that forms a bristle; in severe mutants, the small disorganized actin filament bundles lack structural integrity and allow bristles to bend and branch during extension. Singed protein is also expressed in migratory cells of the developing egg chamber and in the socket cell of the developing bristle, but no defect is observed in these cells in singed mutants. Purified, bacterially expressed singed protein bundles actin filaments in vitro with the same stoichiometry reported for purified sea urchin fascin. Singed-saturated actin bundles have a molar ratio of singed/actin of approximately 1:4.3 and a transverse cross-banding pattern of 12 nm seen using electron microscopy. Our results suggest that singed protein is required for actin filament bundle formation and is a Drosophila homolog of echinoderm fascin.
Appel,
The Drosophila Stubble-stubbloid gene encodes an apparent transmembrane serine protease required for epithelial morphogenesis.
1993, Pubmed
Appel,
The Drosophila Stubble-stubbloid gene encodes an apparent transmembrane serine protease required for epithelial morphogenesis.
1993,
Pubmed
Bender,
Studies on the Expression of Various Singed Alleles in Drosophila Melanogaster.
1960,
Pubmed
Bryan,
Fascin, an echinoid actin-bundling protein, is a homolog of the Drosophila singed gene product.
1993,
Pubmed
,
Echinobase
Bryan,
Separation and interaction of the major components of sea urchin actin gel.
1978,
Pubmed
,
Echinobase
Cooley,
chickadee encodes a profilin required for intercellular cytoplasm transport during Drosophila oogenesis.
1992,
Pubmed
DeRosier,
Structure of actin-containing filaments from two types of non-muscle cells.
1977,
Pubmed
,
Echinobase
DeRosier,
Structure of F-actin needles from extracts of sea urchin oocytes.
1981,
Pubmed
,
Echinobase
Gutzeit,
The role of microfilaments in cytoplasmic streaming in Drosophila follicles.
1986,
Pubmed
Hoover,
Effects of transposable elements on the expression of the forked gene of Drosophila melanogaster.
1993,
Pubmed
Kane,
Actin polymerization and interaction with other proteins in temperature-induced gelation of sea urchin egg extracts.
1976,
Pubmed
,
Echinobase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Maekawa,
A protein in starfish sperm head which bundles actin filaments in vitro: purification and characterization.
1982,
Pubmed
,
Echinobase
Montell,
slow border cells, a locus required for a developmentally regulated cell migration during oogenesis, encodes Drosophila C/EBP.
1992,
Pubmed
Otto,
Formation of filopodia in coelomocytes: localization of fascin, a 58,000 dalton actin cross-linking protein.
1979,
Pubmed
,
Echinobase
Otto,
Redistribution of actin and fascin in sea urchin eggs after fertilization.
1980,
Pubmed
,
Echinobase
Otto,
Assembly-disassembly of actin bundles in starfish oocytes: an analysis of actin-associated proteins in the isolated cortex.
1984,
Pubmed
,
Echinobase
Overton,
The fine structure of developing bristles in wild type and mutant Drosophila melanogaster.
1967,
Pubmed
Paterson,
Structure and transcription of the singed locus of Drosophila melanogaster.
1991,
Pubmed
Perrimon,
Clonal analysis of the tissue specificity of recessive female-sterile mutations of Drosophila melanogaster using a dominant female-sterile mutation Fs(1)K1237.
1983,
Pubmed
Petersen,
forked proteins are components of fiber bundles present in developing bristles of Drosophila melanogaster.
1994,
Pubmed
Roiha,
P element insertions and rearrangements at the singed locus of Drosophila melanogaster.
1988,
Pubmed
Schweisguth,
Suppressor of Hairless, the Drosophila homolog of the mouse recombination signal-binding protein gene, controls sensory organ cell fates.
1992,
Pubmed
Smith,
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
1988,
Pubmed
Spudich,
Structure of actin filament bundles from microvilli of sea urchin eggs.
1979,
Pubmed
,
Echinobase
Spudich,
The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.
1971,
Pubmed
Stokes,
Growth conditions control the size and order of actin bundles in vitro.
1991,
Pubmed
,
Echinobase
Xue,
kelch encodes a component of intercellular bridges in Drosophila egg chambers.
1993,
Pubmed
Yamashiro-Matsumura,
Purification and characterization of an F-actin-bundling 55-kilodalton protein from HeLa cells.
1985,
Pubmed
Yamashiro-Matsumura,
Intracellular localization of the 55-kD actin-bundling protein in cultured cells: spatial relationships with actin, alpha-actinin, tropomyosin, and fimbrin.
1986,
Pubmed