Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Nucleic Acids Res
1994 Mar 25;226:937-45. doi: 10.1093/nar/22.6.937.
Show Gene links
Show Anatomy links
Octamer displacement and redistribution in transcription of single nucleosomes.
O'Donohue MF
,
Duband-Goulet I
,
Hamiche A
,
Prunell A
.
???displayArticle.abstract???
Single nucleosomes were assembled on a 357bp DNA fragment containing a 5S RNA gene from sea urchin and a promoter for SP6 RNA polymerase, and were fractionated as a function of their positions by gel electrophoresis. Transcribed nucleosome positions were detected by observing band disappearance in gels, which in turn provided evidence for the displacement of the histone octamer upon transcription. Differential band disappearance showed that nucleosomes closer to the promoter were harder to transcribe, and transcription was blocked when the nucleosome proximal boundary was at the start site. Nucleosomes located at discrete positions were also eluted from the gel bands and transcribed. In this case, new bands appeared as a consequence of octamer redistribution. Such redistribution occurred over all untranscribed positions, as well as over transcribed positions close enough to the promoter. Similar conclusions were derived from another previously investigated fragment containing a Xenopus 5S RNA gene.
Adams,
Nucleosome displacement in transcription.
1993, Pubmed
Adams,
Nucleosome displacement in transcription.
1993,
Pubmed
Ausio,
Structure and dynamics of transcriptionally active chromatin.
1992,
Pubmed
Bates,
Stability of the higher-order structure of chicken-erythrocyte chromatin in solution.
1981,
Pubmed
Clark,
A nucleosome core is transferred out of the path of a transcribing polymerase.
1992,
Pubmed
Duband-Goulet,
Chromatin reconstitution on small DNA rings. IV. DNA supercoiling and nucleosome sequence preference.
1992,
Pubmed
,
Echinobase
Felsenfeld,
Chromatin as an essential part of the transcriptional mechanism.
1992,
Pubmed
Gould,
An examination of models for chromatin transcription.
1980,
Pubmed
Kirov,
In vitro transcription through nucleosomes by T7 RNA polymerase.
1992,
Pubmed
Kornberg,
Irresistible force meets immovable object: transcription and the nucleosome.
1991,
Pubmed
Kornberg,
Chromatin structure and transcription.
1992,
Pubmed
Lorch,
Nucleosomes inhibit the initiation of transcription but allow chain elongation with the displacement of histones.
1987,
Pubmed
Lorch,
On the displacement of histones from DNA by transcription.
1988,
Pubmed
Losa,
A bacteriophage RNA polymerase transcribes in vitro through a nucleosome core without displacing it.
1987,
Pubmed
,
Echinobase
Meersseman,
Mobile nucleosomes--a general behavior.
1992,
Pubmed
,
Echinobase
Morse,
Nucleosomes inhibit both transcriptional initiation and elongation by RNA polymerase III in vitro.
1989,
Pubmed
Morse,
Transcribed chromatin.
1992,
Pubmed
O'Neill,
Histone octamer dissociation is not required for transcript elongation through arrays of nucleosome cores by phage T7 RNA polymerase in vitro.
1993,
Pubmed
O'Neill,
Nucleosome arrays inhibit both initiation and elongation of transcripts by bacteriophage T7 RNA polymerase.
1992,
Pubmed
,
Echinobase
Prunell,
Periodicity of exonuclease III digestion of chromatin and the pitch of deoxyribonucleic acid on the nucleosome.
1983,
Pubmed
Shrader,
Artificial nucleosome positioning sequences.
1989,
Pubmed
Simpson,
Structural features of a phased nucleosome core particle.
1983,
Pubmed
,
Echinobase
Simpson,
Chromatin reconstituted from tandemly repeated cloned DNA fragments and core histones: a model system for study of higher order structure.
1985,
Pubmed
,
Echinobase
Stein,
DNA folding by histones: the kinetics of chromatin core particle reassembly and the interaction of nucleosomes with histones.
1979,
Pubmed
Thoma,
Structural changes in nucleosomes during transcription: strip, split or flip?
1991,
Pubmed
Voordouw,
Binding of additional histones to chromatin core particles.
1978,
Pubmed
Wasylyk,
Studies on the mechanism of transcription of nucleosomal complexes.
1980,
Pubmed
Wasylyk,
Transcription of in vitro assembled chromatin by Escherichia coli RNA polymerase.
1979,
Pubmed
Williamson,
Transcription of histone-covered T7 DNA by Escherichia coli RNA polymerase.
1978,
Pubmed
Wolffe,
Initiation of transcription on nucleosomal templates.
1989,
Pubmed
Wu,
The locus of sequence-directed and protein-induced DNA bending.
1984,
Pubmed
Zivanovic,
Chromatin reconstitution on small DNA rings. III. Histone H5 dependence of DNA supercoiling in the nucleosome.
1990,
Pubmed
ten Heggeler-Bordier,
The apical localization of transcribing RNA polymerases on supercoiled DNA prevents their rotation around the template.
1992,
Pubmed
van Holde,
What happens to nucleosomes during transcription?
1992,
Pubmed
von Hippel,
Protein-nucleic acid interactions in transcription: a molecular analysis.
1984,
Pubmed
