ECB-ART-35907
J Cell Biol
1994 Nov 01;1274:973-84. doi: 10.1083/jcb.127.4.973.
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Polyribosome targeting to microtubules: enrichment of specific mRNAs in a reconstituted microtubule preparation from sea urchin embryos.
Hamill D
,
Davis J
,
Drawbridge J
,
Suprenant KA
.
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A subset of mRNAs, polyribosomes, and poly(A)-binding proteins copurify with microtubules from sea urchin embryos. Several lines of evidence indicate that the interaction of microtubules with ribosomes is specific: a distinct stalk-like structure appears to mediate their association; ribosomes bind to microtubules with a constant stoichiometry through several purification cycles; and the presence of ribosomes in these preparations depends on the presence of intact microtubules. Five specific mRNAs are enriched with the microtubule-bound ribosomes, indicating that translation of specific proteins may occur on the microtubule scaffolding in vivo.
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Genes referenced: LOC100887844 LOC100893907
References [+] :
Ainger,
Transport and localization of exogenous myelin basic protein mRNA microinjected into oligodendrocytes.
1993, Pubmed
Ainger, Transport and localization of exogenous myelin basic protein mRNA microinjected into oligodendrocytes. 1993, Pubmed
Berleth, The role of localization of bicoid RNA in organizing the anterior pattern of the Drosophila embryo. 1988, Pubmed
Drawbridge, Identification and characterization of the poly(A)-binding proteins from the sea urchin: a quantitative analysis. 1990, Pubmed , Echinobase
Dubé, The hierarchy of requirements for an elevated intracellular pH during early development of sea urchin embryos. 1985, Pubmed , Echinobase
Edgar, Cytoarchitecture and the patterning of fushi tarazu expression in the Drosophila blastoderm. 1987, Pubmed
Etter, Ribosomal heterogeneity from chromatin diminution in Ascaris lumbricoides. 1994, Pubmed
Euteneuer, Polarity of some motility-related microtubules. 1981, Pubmed
Fulton, Spatial organization of the synthesis of cytoskeletal proteins. 1993, Pubmed
Gavis, Localization of nanos RNA controls embryonic polarity. 1992, Pubmed
Gibbons, Dynein: A Protein with Adenosine Triphosphatase Activity from Cilia. 1965, Pubmed
Goldman, The structure and some properties of the isolated mitotic apparatus. 1969, Pubmed
Gottlieb, The 3' untranslated region of localized maternal messages contains a conserved motif involved in mRNA localization. 1992, Pubmed
Goustin, Protein synthesis, polyribosomes, and peptide elongation in early development of Strongylocentrotus purpuratus. 1981, Pubmed , Echinobase
Grainger, Intracellular pH controls protein synthesis rate in the sea urchine egg and early embryo. 1979, Pubmed , Echinobase
Haimo, Dynein-microtubule interactions: ATP-sensitive dynein binding and the structural polarity of mitotic microtubules. 1982, Pubmed
Heuser, Filament organization revealed in platinum replicas of freeze-dried cytoskeletons. 1980, Pubmed
Hille, The protein synthetic machinery: ribosomes and cell-free systems. 1986, Pubmed , Echinobase
Hirokawa, Cytoskeletal architecture of isolated mitotic spindle with special reference to microtubule-associated proteins and cytoplasmic dynein. 1985, Pubmed , Echinobase
Isaacs, Cotranslational assembly of myosin heavy chain in developing cultured skeletal muscle. 1987, Pubmed
Isaacs, Biosynthesis of titin in cultured skeletal muscle cells. 1989, Pubmed
Jeffery, Localized mRNA and the egg cytoskeleton. 1989, Pubmed
Jessus, Interaction between rat brain microtubule associated proteins (MAPs) and free ribosomes from Xenopus oocyte: a possible mechanism for the in ovo distribution of MAPs. 1984, Pubmed
Jessus, In vitro inhibition of tubulin assembly by a ribonucleoprotein complex associated with the free ribosome fraction isolated from Xenopus laevis oocytes: effect at the level of microtubule-associated proteins. 1984, Pubmed
Johnson, Intracellular pH and activation of sea urchin eggs after fertilisation. 1976, Pubmed , Echinobase
Kislauskis, Isoform-specific 3'-untranslated sequences sort alpha-cardiac and beta-cytoplasmic actin messenger RNAs to different cytoplasmic compartments. 1993, Pubmed
Lake, Evolving ribosome structure: domains in archaebacteria, eubacteria, eocytes and eukaryotes. 1985, Pubmed
Lenk, A cytoskeletal structure with associated polyribosomes obtained from HeLa cells. 1977, Pubmed
Loomis, Identification of nuclear tau isoforms in human neuroblastoma cells. 1990, Pubmed
LOWRY, Protein measurement with the Folin phenol reagent. 1951, Pubmed
Lye, Identification of a microtubule-based cytoplasmic motor in the nematode C. elegans. 1987, Pubmed
Macdonald, cis-acting sequences responsible for anterior localization of bicoid mRNA in Drosophila embryos. 1988, Pubmed
Macdonald, RNA regulatory element BLE1 directs the early steps of bicoid mRNA localization. 1993, Pubmed
Martin, Polysome structure in sea urchin eggs and embryos: an electron microscopic analysis. 1983, Pubmed , Echinobase
Mitchison, Dynamic instability of microtubule growth. 1984, Pubmed
Moon, The cytoskeletal framework of sea urchin eggs and embryos: developmental changes in the association of messenger RNA. 1983, Pubmed , Echinobase
Mowry, Vegetal messenger RNA localization directed by a 340-nt RNA sequence element in Xenopus oocytes. 1992, Pubmed
Pachter, Association of mRNA with the cytoskeletal framework: its role in the regulation of gene expression. 1992, Pubmed
Papasozomenos, Phosphorylation determines two distinct species of Tau in the central nervous system. 1987, Pubmed
Paschal, MAP 1C is a microtubule-activated ATPase which translocates microtubules in vitro and has dynein-like properties. 1987, Pubmed
Pelham, An efficient mRNA-dependent translation system from reticulocyte lysates. 1976, Pubmed
Pokrywka, Microtubules mediate the localization of bicoid RNA during Drosophila oogenesis. 1991, Pubmed
Raff, Two distinct mechanisms localise cyclin B transcripts in syncytial Drosophila embryos. 1990, Pubmed
Ramagopal, Are eukaryotic ribosomes heterogeneous? Affirmations on the horizon. 1992, Pubmed
Raymond, Isolation of a 50 kDa polypeptide from the detergent-resistant unfertilized sea urchin egg cytomatrix and evidence for its change in organization during mitosis. 1988, Pubmed , Echinobase
Rebhun, In vitro reactivation of anaphase B in isolated spindles of the sea urchin egg. 1988, Pubmed , Echinobase
Ris, The cytoplasmic filament system in critical point-dried whole mounts and plastic-embedded sections. 1985, Pubmed
Sachs, Messenger RNA degradation in eukaryotes. 1993, Pubmed
Salmon, Mitotic spindles isolated from sea urchin eggs with EGTA lysis buffers. 1982, Pubmed , Echinobase
Salmon, Calcium-labile mitotic spindles isolated from sea urchin eggs (Lytechinus variegatus). 1980, Pubmed , Echinobase
Schatten, Intracellular pH shift leads to microtubule assembly and microtubule-mediated motility during sea urchin fertilization: correlations between elevated intracellular pH and microtubule activity and depressed intracellular pH and microtubule disassembly. 1985, Pubmed , Echinobase
Schatten, The supramolecular organization of the cytoskeleton during fertilization. 1984, Pubmed
Schwartz, A 69-kDa RNA-binding protein from Xenopus oocytes recognizes a common motif in two vegetally localized maternal mRNAs. 1992, Pubmed
Silver, Isolation of mitotic apparatus containing vesicles with calcium sequestration activity. 1980, Pubmed , Echinobase
Singer, The cytoskeleton and mRNA localization. 1992, Pubmed
Steffen, Evidence for a non-tubulin spindle matrix and for spindle components immunologically related to tektin filaments. 1992, Pubmed , Echinobase
Steward, Getting the message from the gene to the synapse: sorting and intracellular transport of RNA in neurons. 1992, Pubmed
St Johnston, Multiple steps in the localization of bicoid RNA to the anterior pole of the Drosophila oocyte. 1989, Pubmed
Suprenant, Assembly of unfertilized sea urchin egg tubulin at physiological temperatures. 1983, Pubmed , Echinobase
Suprenant, Temperature and pH govern the self-assembly of microtubules from unfertilized sea-urchin egg extracts. 1987, Pubmed , Echinobase
Suprenant, Association of ribosomes with in vitro assembled microtubules. 1989, Pubmed , Echinobase
Suprenant, Microtubules, ribosomes, and RNA: evidence for cytoplasmic localization and translational regulation. 1993, Pubmed
Suprenant, EMAP, an echinoderm microtubule-associated protein found in microtubule-ribosome complexes. 1993, Pubmed , Echinobase
Theodorakis, Physical evidence for cotranslational regulation of beta-tubulin mRNA degradation. 1992, Pubmed
Towbin, Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. 1979, Pubmed
Vale, Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility. 1985, Pubmed
Valentine, Regulation of glutamine synthetase. XII. Electron microscopy of the enzyme from Escherichia coli. 1968, Pubmed
Vallee, Purification of microtubules and microtubule-associated proteins from sea urchin eggs and cultured mammalian cells using taxol, and use of exogenous taxol-stabilized brain microtubules for purifying microtubule-associated proteins. 1986, Pubmed , Echinobase
Verschoor, Three-dimensional structure of the mammalian cytoplasmic ribosome. 1990, Pubmed
Weatherbee, Binding of adenovirus to microtubules. II. Depletion of high-molecular-weight microtubule-associated protein content reduces specificity of in vitro binding. 1977, Pubmed
Wilhelm, RNA on the move: the mRNA localization pathway. 1993, Pubmed
Winkler, Multiple levels of regulation of protein synthesis at fertilization in sea urchin eggs. 1985, Pubmed , Echinobase
Wolosewick, Stereo high-voltage electron microscopy of whole cells of the human diploid line, WI-38. 1976, Pubmed
Yisraeli, A two-step model for the localization of maternal mRNA in Xenopus oocytes: involvement of microtubules and microfilaments in the translocation and anchoring of Vg1 mRNA. 1990, Pubmed