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Proc Natl Acad Sci U S A
1995 Mar 14;926:2393-7. doi: 10.1073/pnas.92.6.2393.
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Conserved nucleoprotein structure at the ends of vertebrate and invertebrate chromosomes.
Lejnine S
,
Makarov VL
,
Langmore JP
.
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Eukaryotic chromosomes terminate with telomeres, nucleoprotein structures that are essential for chromosome stability. Vertebrate telomeres consist of terminal DNA tracts of sequence (TTAGGG)n, which in rat are predominantly organized into nucleosomes regularly spaced by 157 bp. To test the hypothesis that telomeres of other animals have nucleosomes, we compared telomeres from eight vertebrate tissues and cell cultures, as well as two tissues from an invertebrate. All telomeres have substantial tracts of (TTAGGG)n comprising 0.01-0.2% of the genome. All telomeres are long (20-100 kb), except for those of sea urchin, human, and some chicken chromosomes, which are 3-10 kb in length. All of the animal telomeres contained nucleosome arrays, consistent with the original hypothesis. The telomere repeat lengths vary from 151 to 205 bp, seemingly uncorrelated with telomere size, regularity of nucleosome spacing, species, or state of differentiation but surprisingly correlated with the repeat of bulk chromatin within the same cells. The telomere nucleosomes were consistently approximately 40 bp smaller than bulk nucleosomes. Thus, animal telomeres have highly conserved sequences and unusually short nucleosomes with cell-specific structure.
Allshire,
Telomeric repeat from T. thermophila cross hybridizes with human telomeres.
1988, Pubmed
Allshire,
Telomeric repeat from T. thermophila cross hybridizes with human telomeres.
1988,
Pubmed
Almouzni,
Nuclear assembly, structure, and function: the use of Xenopus in vitro systems.
1993,
Pubmed
Blackburn,
Structure and function of telomeres.
1991,
Pubmed
Blackburn,
Telomerases.
1992,
Pubmed
Blasquez,
Simian virus 40 protein VP1 is involved in spacing nucleosomes in minichromosomes.
1986,
Pubmed
Conconi,
Two different chromatin structures coexist in ribosomal RNA genes throughout the cell cycle.
1989,
Pubmed
Counter,
Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity.
1992,
Pubmed
de Lange,
Structure and variability of human chromosome ends.
1990,
Pubmed
Dilworth,
Chromatin assembly in vitro and in vivo.
1988,
Pubmed
Drew,
Reconstitution of short-spaced chromatin from the histone octamer and either HMG-14,17 or histone H1.
1993,
Pubmed
German,
The chromosomal complement of blastomeres in Arbacia punctulata.
1966,
Pubmed
,
Echinobase
Harley,
Telomeres shorten during ageing of human fibroblasts.
1990,
Pubmed
Hastie,
Telomere reduction in human colorectal carcinoma and with ageing.
1990,
Pubmed
Kipling,
Hypervariable ultra-long telomeres in mice.
1990,
Pubmed
Leffak,
Chromatin assembled in the presence of cytosine arabinoside has a short nucleosome repeat.
1983,
Pubmed
Makarov,
Nucleosomal organization of telomere-specific chromatin in rat.
1993,
Pubmed
Meyne,
Conservation of the human telomere sequence (TTAGGG)n among vertebrates.
1989,
Pubmed
Moyzis,
A highly conserved repetitive DNA sequence, (TTAGGG)n, present at the telomeres of human chromosomes.
1988,
Pubmed
Nanda,
Localization of the telomeric (TTAGGG)n sequence in chicken (Gallus domesticus) chromosomes.
1994,
Pubmed
Poccia,
Transitions in histone variants during sea urchin spermatogenesis.
1987,
Pubmed
,
Echinobase
RodrÃguez-Campos,
Assembly and properties of chromatin containing histone H1.
1989,
Pubmed
Starling,
Extensive telomere repeat arrays in mouse are hypervariable.
1990,
Pubmed
Stein,
A model chromatin assembly system. Factors affecting nucleosome spacing.
1984,
Pubmed
Tolley,
A high-yield, high-purity elutriation method for preparing human granulocytes demonstrating enhanced experimental lifetimes.
1987,
Pubmed
Tommerup,
Unusual chromatin in human telomeres.
1994,
Pubmed
Tremethick,
Partial purification, from Xenopus laevis oocytes, of an ATP-dependent activity required for nucleosome spacing in vitro.
1992,
Pubmed
Tremethick,
High mobility group proteins 14 and 17 can space nucleosomes in vitro.
1993,
Pubmed
Vincenz,
Nucleoprotein hybridization: a method for isolating active and inactive genes as chromatin.
1991,
Pubmed
,
Echinobase
Wu,
The chromatin structure of specific genes: II. Disruption of chromatin structure during gene activity.
1979,
Pubmed
Zakian,
Structure and function of telomeres.
1989,
Pubmed
