Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Biochem J
1994 Dec 15;304 ( Pt 3):911-6. doi: 10.1042/bj3040911.
Show Gene links
Show Anatomy links
Identification and partial sequence analysis of novel annexins in Lytechinus pictus oocytes.
Shen WJ
,
Avery J
,
Totty NF
,
Hsuan JJ
,
Whitaker M
,
Moss SE
.
???displayArticle.abstract???
The annexins are a major class of calcium-binding proteins with unknown functions. In an attempt to define novel model systems in which to study members of the annexin family, we have investigated the expression of annexins in eggs from the sea urchin Lytechinus pictus. Western blot analysis of L. pictus eggs using antisera raised against human annexins I, V and VI revealed the presence of immunoreactive proteins of approximately 34 kDa, 35 kDa and 68 kDa respectively. The sea urchin annexins behaved similarly to their mammalian counterparts, both during purification and in their ability to bind calcium-dependently to anionic phospholipids. Of the three sea urchin annexins, the 34 kDa form was most abundant, yielding sufficient quantities for peptide microsequencing. The amino acid sequences derived in this way showed the L. pictus annexin to be closely related both to mammalian annexin I and to annexins IX, X and XII from Drosophila and Hydra. However, N-terminal sequence from the L. pictus annexin showed it to be a novel member of the annexin super-gene family. The results are interesting in view of the complex evolution of the annexin gene family, and also point to the potential usefulness of echinoderm eggs as a model system in which to study annexin function.
Ali,
A role for calpactin in calcium-dependent exocytosis in adrenal chromaffin cells.
1989, Pubmed
Ali,
A role for calpactin in calcium-dependent exocytosis in adrenal chromaffin cells.
1989,
Pubmed
Baker,
Influence of ATP and calcium on the cortical reaction in sea urchin eggs.
1978,
Pubmed
,
Echinobase
Chiang,
Annexin II expression is regulated during mammalian cell cycle.
1993,
Pubmed
Creutz,
Differential recognition of secretory vesicles by annexins. European Molecular Biology Organization Course "Advanced Techniques for Studying Secretion".
1992,
Pubmed
Creutz,
The annexins and exocytosis.
1992,
Pubmed
Crompton,
Diversity in the lipocortin/calpactin family.
1988,
Pubmed
Davidson,
Biological relevance of lipocortins and related proteins as inhibitors of phospholipase A2.
1989,
Pubmed
Döring,
Dictyostelium annexin VII (synexin). cDNA sequence and isolation of a gene disruption mutant.
1991,
Pubmed
Drust,
Aggregation of chromaffin granules by calpactin at micromolar levels of calcium.
1988,
Pubmed
Edwards,
Ca(2+)-dependent phospholipid and arachidonic acid binding by the placental annexins VI and IV.
1991,
Pubmed
Emans,
Annexin II is a major component of fusogenic endosomal vesicles.
1993,
Pubmed
Gerke,
Consensus peptide antibodies reveal a widespread occurrence of Ca2+/lipid-binding proteins of the annexin family.
1989,
Pubmed
Hitti,
Structure of the gene encoding columbid annexin Icp35.
1991,
Pubmed
Huber,
The crystal and molecular structure of human annexin V, an anticoagulant protein that binds to calcium and membranes.
1990,
Pubmed
Johnston,
Two novel annexins from Drosophila melanogaster. Cloning, characterization, and differential expression in development.
1990,
Pubmed
Keutzer,
The growth-regulated gene 1B6 is identified as the heavy chain of calpactin I.
1990,
Pubmed
Kovacic,
Correlation of gene and protein structure of rat and human lipocortin I.
1991,
Pubmed
Lin,
Annexin VI is required for budding of clathrin-coated pits.
1992,
Pubmed
Maekawa,
Two Ca2(+)-binding proteins in the mitotic apparatus of sea urchin eggs.
1991,
Pubmed
,
Echinobase
Moss,
A growth-dependent post-translational modification of annexin VI.
1992,
Pubmed
Moss,
Molecular cloning of murine p68, a Ca2+-binding protein of the lipocortin family.
1988,
Pubmed
Robitzki,
cDNA structure and expression of calpactin, a peptide involved in Ca2(+)-dependent cell aggregation in sponges.
1990,
Pubmed
Schlaepfer,
Inhibition of protein kinase C by annexin V.
1992,
Pubmed
Schlaepfer,
Identification of a novel annexin in Hydra vulgaris. Characterization, cDNA cloning, and protein kinase C phosphorylation of annexin XII.
1992,
Pubmed
Smith,
Structure of the human annexin VI gene.
1994,
Pubmed
Smith,
Structural evolution of the annexin supergene family.
1994,
Pubmed
Smythe,
Endocytosis occurs independently of annexin VI in human A431 cells.
1994,
Pubmed
Spano,
Characterization of the human lipocortin-2-encoding multigene family: its structure suggests the existence of a short amino acid unit undergoing duplication.
1990,
Pubmed
Tait,
Placental anticoagulant proteins: isolation and comparative characterization four members of the lipocortin family.
1988,
Pubmed
Tait,
Structure and polymorphisms of the human annexin III (ANX3) gene.
1993,
Pubmed
Totty,
Accelerated high-sensitivity microsequencing of proteins and peptides using a miniature reaction cartridge.
1992,
Pubmed
Vacquier,
The isolation of intact cortical granules from sea urchin eggs: calcium lons trigger granule discharge.
1975,
Pubmed
,
Echinobase
Whalley,
Phosphoprotein inhibition of calcium-stimulated exocytosis in sea urchin eggs.
1991,
Pubmed
,
Echinobase
