Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
???displayArticle.abstract???
B(alpha beta) tubulin was obtained from a homogeneous class of microtubules, the incomplete B subfiber of sea urchin sperm flagellar doublet microtubules, by thermal fractionation. The thermally derived soluble B tubulin fraction (100, 000 g-h) repolymerizes in vitro, yielding microtubule-like structures. The microtubule-associated protein (MAP) composition and certain assembly parameters of thermally derived B tubulin are different from those reported for sonication-derived flageller tubulin and purified vertebrate tubulin. The "microtubules" reassembled from thermally prepared B tubulin are composed of 12-15 protofilaments (73% possess 14 protofilaments). A certain number possess a single "adlumenal component" applied to their inside walls, regardless of the number of protofilaments. Following the first cycle of polymerization, 81% of the B tubulin and essentially 100% of the MAPs remain cold insoluble. Evidence suggests that B tubulin assembles faithfully into a B lattice, creating a j seam between two protofilaments that are laterally bonded in a A-lattice configuration. The significance of these seams is discussed in relation to the mechanism of microtubule assembly, the stability of observed ribbons of protofilaments, and the three-dimensional organization of microtubule-associated components.
Amos,
Arrangement of subunits in flagellar microtubules.
1974, Pubmed
Amos,
Arrangement of subunits in flagellar microtubules.
1974,
Pubmed
Bell,
Polypeptide subunits of dynein 1 from sea urchin sperm flagella.
1979,
Pubmed
,
Echinobase
Berkowitz,
Separation and characterization of microtubule proteins from calf brain.
1977,
Pubmed
Bibring,
Heterogeneity of the alpha subunit of tubulin and the variability of tubulin within a single organism.
1976,
Pubmed
,
Echinobase
Binder,
The in vitro assembly of flagellar outer doublet tubulin.
1978,
Pubmed
Bryan,
Biochemical properties of microtubules.
1974,
Pubmed
,
Echinobase
CASPAR,
Physical principles in the construction of regular viruses.
1962,
Pubmed
Cleveland,
Physical and chemical properties of purified tau factor and the role of tau in microtubule assembly.
1977,
Pubmed
Cleveland,
Purification of tau, a microtubule-associated protein that induces assembly of microtubules from purified tubulin.
1977,
Pubmed
Crepeau,
Differences in alpha and beta polypeptide chains of tubulin resolved by electron microscopy with image reconstruction.
1978,
Pubmed
Dentler,
Ultrastructural localization of the high molecular weight proteins associated with in vitro-assembled brain microtubules.
1975,
Pubmed
Erickson,
Microtubule surface lattice and subunit structure and observations on reassembly.
1974,
Pubmed
Farrell,
Characterization of the in vitro reassembly of tubulin derived from stable Strongylocentrotus purpuratus outer doublet microtubules.
1979,
Pubmed
,
Echinobase
Farrell,
Microtubule reassembly in vitro of Strongylocentrotus purpuratus sperm tail outer doublet tubulin.
1978,
Pubmed
,
Echinobase
Gaskin,
Turbidimetric studies of the in vitro assembly and disassembly of porcine neurotubules.
1974,
Pubmed
Gibbons,
Chemical dissection of cilia.
1965,
Pubmed
Gibbons,
A latent adenosine triphosphatase form of dynein 1 from sea urchin sperm flagella.
1979,
Pubmed
,
Echinobase
Haimo,
Dynein binds to and crossbridges cytoplasmic microtubules.
1979,
Pubmed
Kirschner,
Microtubule assembly and nucleation.
1978,
Pubmed
Kuriyama,
In vitro polymerization of flagellar and ciliary outer fiber tubulin into microtubules.
1976,
Pubmed
,
Echinobase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Langford,
In vitro assembly of dogfish brain tubulin and the induction of coiled ribbon polymers by calcium.
1978,
Pubmed
Langford,
Arrangement of subunits in microtubules with 14 profilaments.
1980,
Pubmed
Ledbetter,
Morphology of Microtubules of Plant Cell.
1964,
Pubmed
Lee,
In vitro reconstitution of calf brain microtubules: effects of solution variables.
1977,
Pubmed
Lee,
In vitro reconstitution of calf brain microtubules: effects of macromolecules.
1978,
Pubmed
Lee,
The chemical characterization of calf brain microtubule protein subunits.
1973,
Pubmed
Linck,
The hands of helical lattices in flagellar doublet microtubules.
1974,
Pubmed
Linck,
Arrangement of tubulin subunits and microtubule-associated proteins in the central-pair microtubule apparatus of squid (Loligo pealei) sperm flagella.
1981,
Pubmed
Linck,
Chemical and structural differences between cilia and flagella from the lamellibranch mollusc, Aequipecten irradians.
1973,
Pubmed
Linck,
Flagellar doublet microtubules: fractionation of minor components and alpha-tubulin from specific regions of the A-tubule.
1976,
Pubmed
,
Echinobase
Little,
Identification of a second beta chain in pig brain tubulin.
1979,
Pubmed
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
Ludueńa,
Structure of the tubulin dimer.
1977,
Pubmed
Mandelkow,
Tubulin hoops.
1977,
Pubmed
Mandelkow,
Junctions between microtubule walls.
1979,
Pubmed
Mandelkow,
Correlation between structural polarity and polar assembly of brain tubulin.
1979,
Pubmed
Mandelkow,
Microtubule structure at low resolution by x-ray diffraction.
1977,
Pubmed
McEwen,
Evidence for a mixed lattice in microtubules reassembled in vitro.
1980,
Pubmed
Meza,
Chemical heterogeneity of protofilaments forming the outer doublets from sea urchin flagella.
1972,
Pubmed
,
Echinobase
Murphy,
Association of high-molecular-weight proteins with microtubules and their role in microtubule assembly in vitro.
1975,
Pubmed
Murphy,
Role of tubulin-associated proteins in microtubule nucleation and elongation.
1977,
Pubmed
Olmsted,
Microtuble assembly: some possible regulatory mechanisms.
1974,
Pubmed
Olmsted,
Characterization of microtubule assembly in porcine brain extracts by viscometry.
1973,
Pubmed
Olson,
Observations of the structural components of flagellar axonemes and central pair microtubules from rat sperm.
1977,
Pubmed
Pierson,
Wall substructure of microtubules polymerized in vitro from tubulin of crayfish nerve cord and fixed with tannic acid.
1979,
Pubmed
Pierson,
Alterations in number of protofilaments in microtubules assembled in vitro.
1978,
Pubmed
Pollard,
Actin and myosin and cell movement.
1974,
Pubmed
Sandoval,
Different tubulin polymers are produced by microtubule-associated proteins MAP2 and tau in the presence of guanosine 5'-(alpha, beta-methylene)triphosphate.
1980,
Pubmed
Sandoval,
Polymerization of tubulin in the presence of colchicine or podophyllotoxin. Formation of a ribbon structure induced by guanylyl-5'-methylene diphosphonate.
1979,
Pubmed
Shapiro,
Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels.
1967,
Pubmed
Sloboda,
Cyclic AMP-dependent endogenous phosphorylation of a microtubule-associated protein.
1975,
Pubmed
Stephens,
Microtubules: structure, chemistry, and function.
1976,
Pubmed
,
Echinobase
Stephens,
Structural chemistry of the axoneme: evidence for chemically and functionally unique tubulin dimers in outer fibers.
1975,
Pubmed
,
Echinobase
Stephens,
Thermal fractionation of outer fiber doublet microtubules into A- and B-subfiber components. A- and B-tubulin.
1970,
Pubmed
Tilney,
Microtubules: evidence for 13 protofilaments.
1973,
Pubmed
,
Echinobase
Ullmann,
On the determination of molecular weight of proteins and protein subunits in the presence of 6 M guanidine hydrochloride.
1968,
Pubmed
Warner,
The substructure of ciliary microtubules.
1973,
Pubmed
Webb,
Cold-stable microtubules from brain.
1980,
Pubmed
Weingarten,
A protein factor essential for microtubule assembly.
1975,
Pubmed
Witman,
Chlamydomonas flagella. II. The distribution of tubulins 1 and 2 in the outer doublet microtubules.
1972,
Pubmed
Witman,
Chlamydomonas flagella. I. Isolation and electrophoretic analysis of microtubules, matrix, membranes, and mastigonemes.
1972,
Pubmed
