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Secondary and tertiary structural differences between histone H1 molecules from calf thymus and sea-urchin (Sphaerechinus granularis) sperm.
Giancotti V
,
Russo E
,
Cosimi S
,
Cary PD
,
Crane-Robinson C
.
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Tryptic digestion of histone H1 from the sperm of the sea urchin Sphaerechinus granularis leaves a limiting peptide of approx. 80 residues that is of similar size to the limit peptide from calf thymus H1 or chicken erythrocyte H5. The S. granularis limit peptide folds to form tertiary structure similar to that of the intact parent histone H1 (shown by n.m.r. spectra), but the helical content is decreased by the digestion from 64 residues to 28. In contrast, intact calf thymus H1 and chicken erythrocyte H5 histones have only about 28 helical residues, which are preserved in their limit peptides. The extra helix in S. granularis is shown to be rapidly digested away by trypsin, and its location in histone H1 is discussed. A possible relationship of this structural feature to the length of linker DNA is proposed.
Allan,
The structure of histone H1 and its location in chromatin.
1980, Pubmed
Allan,
The structure of histone H1 and its location in chromatin.
1980,
Pubmed
Aviles,
The conformation of histone H5. Isolation and characterisation of the globular segment.
1978,
Pubmed
Barbero,
Structural studies on histones H1. Circular dichroism and difference spectroscopy of the histones H1 and their trypsin-resistant cores from calf thymus and from the fruit fly Ceratitis capitata.
1980,
Pubmed
Böhm,
Purification of the five main calf thymus histone fractions by gel exclusion chromatography.
1973,
Pubmed
Bradbury,
Studies on the role and mode of operation of the very-lysine-rich histone H1 (F1) in eukaryote chromatin. The conformation of histone H1.
1975,
Pubmed
Chen,
Determination of the helix and beta form of proteins in aqueous solution by circular dichroism.
1974,
Pubmed
Giancotti,
Tyrosine fluorescence of two tryptophan-free proteins: histones H1 and H5.
1977,
Pubmed
Giancotti,
Preparation and characterization of histone H1 from the sperm of the sea-urchin Sphaerechinus granularis.
1981,
Pubmed
,
Echinobase
Hartman,
Studies on the role and mode of operation of the very-lysine-rich histone H1 in eukaryote chromatin. The three structural regions of the histone H1 molecule.
1977,
Pubmed
Johns,
Studies on histones. 7. Preparative methods for histone fractions from calf thymus.
1964,
Pubmed
Moss,
A pH-dependent interaction between histones H2A and H2B involving secondary and tertiary folding.
1976,
Pubmed
Noll,
Differences and similarities in chromatin structure of Neurospora crassa and higher eucaryotes.
1976,
Pubmed
Puigdomenech,
The structure of sea-urchin-sperm histone phi 1 (H1) in chromatin and in free solution. Trypsin digestion and spectroscopic studies.
1980,
Pubmed
,
Echinobase
Smerdon,
Interactions between the subfractons of calf thymus H1 and nonhistone chromosomal proteins HMG1 and HMG2.
1976,
Pubmed
Spadafora,
The DNA repeat lengths in chromatins from sea urchin sperm and gastrule cells are markedly different.
1976,
Pubmed
,
Echinobase
Weintraub,
The nucleosome repeat length increases during erythropoiesis in the chick.
1978,
Pubmed
