Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
???displayArticle.abstract???
The role of cell surface glycoproteins of the sea urchin egg in binding sperm has been examined by studying the biological activity of glycopeptides derived from these glycoproteins. Glycopeptides were produced from egg surface glycoproteins by Pronase digestion. After fractionation by gel filtration the glycopeptides were tested for their ability to inhibit the binding of sperm to eggs, presumably by competing with the egg surface glycoproteins for binding sites on the sperm. One glycopeptide fraction with an apparent molecular weight of approximately 6,000 was found to be a potent inhibitor of sperm-egg binding, as well as fertilization, even at nanomolar concentrations. This activity was heat stable and exerted its effect against the sperm and not the egg. Experiments with a radiolabeled form of the glycopeptide fraction directly demonstrated that at least one component of it bound to sperm. Specific binding of the radiolabeled glycopeptide occurred only to acrosome-reacted sperm. Because the isolated glycopeptide fraction has many of the characteristics that one would expect of a biologically active fragment of an egg surface receptor for sperm, these findings are consistent with the idea that one or more glycoconjugates on the surface of the egg are involved in sperm binding.
Aketa,
Tryptic disruption of sperm-binding site of sea urchin egg surface.
1972, Pubmed,
Echinobase
Aketa,
Tryptic disruption of sperm-binding site of sea urchin egg surface.
1972,
Pubmed
,
Echinobase
Aketa,
A sperm factor as the counterpart to the sperm-binding factor of the homologous eggs.
1978,
Pubmed
,
Echinobase
Bellet,
Characterization and comparison of "bindin" isolated from sperm of two species of sea urchins.
1977,
Pubmed
,
Echinobase
Bleil,
Mammalian sperm-egg interaction: identification of a glycoprotein in mouse egg zonae pellucidae possessing receptor activity for sperm.
1980,
Pubmed
Bolwell,
Fertilization in brown algae. III. Preliminary characterization of putative gamete receptors from eggs and sperm of Fucus serratus.
1980,
Pubmed
Carroll,
Isolation and biological activity of the proteases released by sea urchin eggs following fertilization.
1975,
Pubmed
,
Echinobase
Decker,
A study of factors involved in induction of the acrosomal reaction in sperm of the sea urchin, Arbacia punctulata.
1976,
Pubmed
,
Echinobase
Glabe,
Species specific agglutination of eggs by bindin isolated from sea urchin sperm.
1977,
Pubmed
,
Echinobase
Glabe,
Species-specific sperm adhesion in sea urchins. A quantitative investigation of bindin-mediated egg agglutination.
1979,
Pubmed
,
Echinobase
Glabe,
Egg surface glycoprotein receptor for sea urchin sperm bindin.
1978,
Pubmed
,
Echinobase
Jourdian,
The sialic acids. XI. A periodate-resorcinol method for the quantitative estimation of free sialic acids and their glycosides.
1971,
Pubmed
Kinsey,
Studies on the specificity of sperm binding in echinoderm fertilization.
1980,
Pubmed
,
Echinobase
Kinsey,
The effect of the acrosome reaction on the respiratory activity and fertilizing capacity of echinoid sperm.
1979,
Pubmed
,
Echinobase
Lallier,
Effects of concanavalin A on the development of sea urchin egg.
1972,
Pubmed
,
Echinobase
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
Means,
Reductive alkylation of amino groups in proteins.
1968,
Pubmed
Misaki,
Glycosyl moiety of the lima bean lectin.
1977,
Pubmed
Nexł,
Lectin-agarose immobilization, a new method for detecting soluble membrane receptors. Application to studies with epidermal growth factor-urogastrone and transcobalamin-II.
1979,
Pubmed
Schmell,
Identification of a sperm receptor on the surface of the eggs of the sea urchin Arbacia punctulata.
1977,
Pubmed
,
Echinobase
Summers,
Primary gamete binding. Quantitative determination of its specificity in echinoid fertilization.
1976,
Pubmed
,
Echinobase
Summers,
Species-specificity of acrosome reaction and primary gamete binding in echinoids.
1975,
Pubmed
,
Echinobase
SVENNERHOLM,
The determination of hexosamines with special reference to nervous tissue.
1957,
Pubmed
Tsuzuki,
Purification of the sperm-binding factor from the egg of the sea urchin, Hemicentrotus pulcherrimus.
1976,
Pubmed
,
Echinobase
Vacquier,
Protease activity establishes the block against polyspermy in sea urchin eggs.
1972,
Pubmed
,
Echinobase
Vacquier,
Isolation of bindin: the protein responsible for adhesion of sperm to sea urchin eggs.
1977,
Pubmed
,
Echinobase
Vacquier,
Methods for quantitating sea urchin sperm-egg binding.
1973,
Pubmed
,
Echinobase
