ECB-ART-35077
Prep Biochem
1983 Jan 01;131:21-39. doi: 10.1080/00327488308068732.
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Purification and characterization of the RNA polymerases of the sea urchin, Lytechinus variegatus.
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We have identified four forms of sea urchin RNA polymerase (Ia, Ib, II and III). Three of the forms co-elute on DEAE cellulose chromatography but separate on DEAE Sephadex chromatography. The separation of these three enzyme forms by DEAE Sephadex chromatography can be eliminated with non-ionic detergent. We also demonstrate that either form I or form III RNA polymerase loses its resistance to alpha-amanitin after DEAE chromatography. A procedure for the purification of combined form I and III RNA polymerase and the purification of RNA polymerase II is also presented.
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Genes referenced: btaf1 LOC100887844 polr3a