ECB-ART-35002
Biochim Biophys Acta
1980 Jul 29;6082:287-94. doi: 10.1016/0005-2787(80)90174-4.
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Characterization of DNA polymerases in mature sperm of the sea urchin.
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In the extract of mature sperm of the sea urchin, Hemicentrotus pulcherrimus, two species of DNA polymerase (deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase, EC 2.7.7.7.) activity were detected. One of them was eluted from a hydroxyapatite column at 0.08 M phosphate buffer and was insensitive to both N-ethylmaleimide and aphidicolin. The sedimentation coefficient was 3.3 S and the isoelectric point was pH 8.6. This activity corresponds to the DNA polymerase-beta activity. The other was eluted from the hydroxyapatite column at 0.14 M phosphate buffer and was inhibited by N-ethylmaleimide but not by aphidicolin. The sedimentation coefficient was 3.3 S and 6.0 S and the isoelectric point was pH 4.8. The template primer preference of this enzyme was coincident with DNA polymerase-gamma. The DNA polymerase-alpha activity was not detected in the extract of mature sperm.
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Genes referenced: LOC100887844 LOC105447382 LOC115923832 LOC586304 polr3a