Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Echinobase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Echinobase
ECB-ART-34991
J Biochem 1984 Apr 01;954:1187-92. doi: 10.1093/oxfordjournals.jbchem.a134708.
Show Gene links Show Anatomy links

Purification and properties of a DNA ligase from sea urchin embryos.

Oishi N , Shimada H .


???displayArticle.abstract???
DNA ligase was purified about 2,000-fold from blastulae of sea urchin, Hemicentrotus pulcherrimus, by means of 1 M KCl-extraction, phosphocellulose, DEAE-cellulose, Sepharose CL-6B, and double-stranded DNA cellulose column chromatography. The purified DNA ligase had a molecular weight of 80,000 (determined by Sephadex G-150) and a sedimentation coefficient of 4.1S (by glycerol gradient centrifugation). The purified enzyme required ATP and Mg2+ (or Mn2+) as cofactors for activity, and was inhibited by N-ethylmaleimide. Apparent Km values for ATP, Mg2+, and Mn2+ were 4 microM, 2.7 mM, and 0.3 mM, respectively.

???displayArticle.pubmedLink??? 6746596
???displayArticle.link??? J Biochem


Genes referenced: LOC100887844