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J Cell Biol
1984 Nov 01;995:1878-82. doi: 10.1083/jcb.99.5.1878.
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Calcium transients during early development in single starfish (Asterias forbesi) oocytes.
Eisen A
,
Reynolds GT
.
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Maturation and fertilization of the starfish oocyte are putative calcium-dependent events. We have investigated the spatial distribution and temporal dynamics of this calcium dependence in single oocytes of Asterias forbesi. We used the calcium photoprotein, aequorin, in conjunction with a microscope-photomultiplier and microscope-image intensifier. Surprisingly, in contrast to earlier work with Marasthenias glacialis, there is no detectable increase in intracellular-free calcium in the oocyte of A. forbesi in response to the maturation hormone 1-methyl adenine. During fertilization of the same, matured, A. forbesi oocyte there is a large increase in intracellular-free calcium. The calcium concentration increases to approximately 1 microM at the point of insemination and the region of elevated free calcium expands across the oocyte in approximately 20 s (17-19 degrees C). After the entire oocyte reaches an elevated concentration of free calcium, the concentration decreases uniformly throughout the oocyte over the next several minutes.
Blinks,
Photoproteins as biological calcium indicators.
1976, Pubmed
Blinks,
Photoproteins as biological calcium indicators.
1976,
Pubmed
Dorée,
Calcium-mediated transduction of the hormonal message in meiosis reinitiation of starfish oocytes: modulation following injection of cholera toxin and cAMP-dependent protein kinase.
1981,
Pubmed
,
Echinobase
Dorée,
Hormonal control of meiosis. In vitro induced release of calcium ions from the plasma membrane in starfish oocytes.
1978,
Pubmed
,
Echinobase
Gilkey,
A free calcium wave traverses the activating egg of the medaka, Oryzias latipes.
1978,
Pubmed
,
Echinobase
Kiehart,
Studies on the in vivo sensitivity of spindle microtubules to calcium ions and evidence for a vesicular calcium-sequestering system.
1981,
Pubmed
,
Echinobase
Masui,
Oocyte maturation.
1979,
Pubmed
Meijer,
Calmodulin in starfish oocytes. I. Calmodulin antagonists inhibit meiosis reinitiation.
1981,
Pubmed
,
Echinobase
Meijer,
Calmodulin in starfish oocytes. II. Trypsin treatment suppresses the trifluoperazine-sensitive step.
1984,
Pubmed
,
Echinobase
Meijer,
Maturation and fertilization in starfish oocytes.
1984,
Pubmed
,
Echinobase
Moreau,
Hormone-induced release of intracellular Ca2+ triggers meiosis in starfish oocytes.
1978,
Pubmed
,
Echinobase
Picard,
Is calcium the second messenger of 1-methyladenine in meiosis reinitiation of starfish oocytes?
1983,
Pubmed
,
Echinobase
Reynolds,
Image intensification applied to biological problems.
1972,
Pubmed
Schuetz,
Cytoplasmic activation of starfish oocytes by sperm and divalent ionophore A-23187.
1975,
Pubmed
,
Echinobase
Shimomura,
EDTA-binding and acylation of the Ca2+-sensitive photoprotein aequorin.
1982,
Pubmed
SHIMOMURA,
Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea.
1962,
Pubmed
