Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Nucleic Acids Res
1984 Feb 10;123:1737-47. doi: 10.1093/nar/12.3.1737.
Show Gene links
Show Anatomy links
Nucleotide sequence of the 18S-26S rRNA intergene region of the sea urchin.
Hindenach BR
,
Stafford DW
.
???displayArticle.abstract???
The DNA sequence which spans the internal transcribed spacers of a cloned ribosomal transcription unit from the sea urchin, Lytechinus variegatus, has been determined. The region extends from the conserved Eco RI site near the 3'' end of the 18S rDNA to a Bam HI site in the 26S rDNA and includes 232 nucleotides coding for 18S rRNA, 367 nucleotides of internal transcribed spacer, 159 nucleotides coding for 5.8S rRNA, 338 nucleotides of internal transcribed spacer, and 505 nucleotides coding for 26S rRNA. The rRNA coding regions were identified by direct analysis of 3''-labeled 18S and 5.8S rRNA and 5''-labeled 5.8S rRNA, and by sequence homology of the 26S rDNA with yeast and vertebrate 26/28S rRNAs. The internal transcribed spacers are GC-rich, similar to those of vertebrates. The 5.8S and 5'' 26S rDNA sequences support a proposed model for a structural domain of the yeast large subunit ribosomal RNA (Veldman et al. [1981] Nucleic Acids Res. 9, 6935-6952).
Azad,
The 3'-terminal primary structure of five eukaryotic 18S rRNAs determined by the direct chemical method of sequencing. The highly conserved sequences include an invariant region complementary to eukaryotic 5S rRNA.
1980, Pubmed
Azad,
The 3'-terminal primary structure of five eukaryotic 18S rRNAs determined by the direct chemical method of sequencing. The highly conserved sequences include an invariant region complementary to eukaryotic 5S rRNA.
1980,
Pubmed
Blin,
Organization of the ribosomal RNA gene cluster in Lytechinus variegatus. Restriction analysis and cloning of restriction fragments.
1979,
Pubmed
,
Echinobase
Brownlee,
The sequence of 5 s ribosomal ribonucleic acid.
1968,
Pubmed
Challberg,
Specific labeling of 3' termini with T4 DNA polymerase.
1980,
Pubmed
Colman,
Rapid purification of plasmid DNAs by hydroxyapatite chromatography.
1978,
Pubmed
Erdmann,
Collection of published 5S and 5.8S ribosomal RNA sequences.
1983,
Pubmed
Eshaghpour,
Preparative separation of the complementary strands of DNA restriction fragments by alkaline RPC-5 chromatography.
1978,
Pubmed
Fujiwara,
Primary and secondary structures of Tetrahymena and aphid 5.8S rRNAs: structural features of 5.8S rRNA which interacts with the 28S rRNA containing the hidden break.
1982,
Pubmed
Furlong,
Patterns of major divergence between the internal transcribed spacers of ribosomal DNA in Xenopus borealis and Xenopus laevis, and of minimal divergence within ribosomal coding regions.
1983,
Pubmed
Georgiev,
The structure of the yeast ribosomal RNA genes. 4. Complete sequence of the 25 S rRNA gene from Saccharomyces cerevisae.
1981,
Pubmed
Griffith,
Ribosomal ribonucleic acid synthesis and processing in embryos of the hawaiian sea urchin Tripneustes gratilla.
1979,
Pubmed
,
Echinobase
Hagenbüchle,
Conservation of the primary structure at the 3' end of 18S rRNA from eucaryotic cells.
1978,
Pubmed
Hall,
Nucleotide sequence through the 18S-28S intergene region of a vertebrate ribosomal transcription unit.
1980,
Pubmed
Jordan,
Coding and spacer sequences in the 5.8S-2S region of Sciara coprophila ribosomal DNA.
1980,
Pubmed
Michot,
Sequence and secondary structure of mouse 28S rRNA 5'terminal domain. Organisation of the 5.8S-28S rRNA complex.
1982,
Pubmed
Michot,
Structure of mouse rRNA precursors. Complete sequence and potential folding of the spacer regions between 18S and 28S rRNA.
1983,
Pubmed
Otsuka,
Nucleotide sequence of Physarum polycephalum 5.8S rRNA gene and its flanking regions.
1982,
Pubmed
Otsuka,
Complete nucleotide sequence of the 26S rRNA gene of Physarum polycephalum: its significance in gene evolution.
1983,
Pubmed
Peattie,
Direct chemical method for sequencing RNA.
1979,
Pubmed
Perry,
Processing of RNA.
1976,
Pubmed
Reddy,
The nucleotide sequence of 8 S RNA bound to preribosomal RNA of Novikoff hepatoma. The 5'-end of 8 S RNA is 5.8 S RNA.
1983,
Pubmed
Subrahmanyam,
Nucleotide sequence of the region between the 18S rRNA sequence and the 28S rRNA sequence of rat ribosomal DNA.
1982,
Pubmed
Veldman,
Some characteristics of processing sites in ribosomal precursor RNA of yeast.
1980,
Pubmed
Veldman,
The nucleotide sequence of the intergenic region between the 5.8S and 26S rRNA genes of the yeast ribosomal RNA operon. Possible implications for the interaction between 5.8S and 26S rRNA and the processing of the primary transcript.
1981,
Pubmed
Veldman,
The primary and secondary structure of yeast 26S rRNA.
1981,
Pubmed
Walker,
Location of 5.8 S rRNA contact sites in 28 S rRNA and the effect of alpha-sarcin on the association of 5.8 S rRNA with 28 S rRNA.
1983,
Pubmed
Wildeman,
Studies on the secondary structure of 5.8 S rRNA from a thermophile, Thermomyces lanuginosus.
1981,
Pubmed
