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Nucleic Acids Res
1982 Dec 20;1024:7977-91. doi: 10.1093/nar/10.24.7977.
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Chromatin structure of histone genes in sea urchin sperms and embryos.
Spinelli G
,
Albanese I
,
Anello L
,
Ciaccio M
,
Di Liegro I
.
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The nucleosomal organization of active and repressed alpha subtype histone genes has been investigated by micrococcal nuclease digestion of P. lividus sperm, 32-64 cell embryo and mesenchyme blastula nuclei, followed by hybridization with 32P-labeled specific DNA probes. In sperms, fully repressed histone genes are regularly folded in nucleosomes, and exhibit a greater resistance to micrococcal nuclease cleavage than bulk chromatin. In contrast, both coding and spacer alpha subtype histone DNA sequences acquire an altered conformation in nuclei from early cleavage stage embryos, i.e., when these genes are maximally expressed. Switching off of the alpha subtype histone genes, in mesenchyme blastulae, restores the typical nucleosomal organization on this chromatin region. As probed by hybridization to D.melanogaster actin cDNA, actin genes retain a regular nucleosomal structure in all the investigated stages.
Albanese,
Unusual properties of sea urchin unfertilized egg chromatin.
1980,
Pubmed
,
Echinobase
Arceci,
Histone variants and chromatin structure during sea urchin development.
1980,
Pubmed
,
Echinobase
Bellard,
Disruption of the typical chromatin structure in a 2500 base-pair region at the 5' end of the actively transcribed ovalbumin gene.
1982,
Pubmed
Bellard,
Differential nuclease sensitivity of the ovalbumin and beta-globin chromatin regions in erythrocytes and oviduct cells of laying hen.
1980,
Pubmed
Bryan,
Nucleosome arrangement on tRNA genes of Xenopus laevis.
1981,
Pubmed
Camerini-Otero,
Nucleosomal packaging of the thymidine kinase gene of herpes simplex virus transferred into mouse cells: an actively expressed single-copy gene.
1980,
Pubmed
Childs,
Histone gene expression during sea urchin embryogenesis: isolation and characterization of early and late messenger RNAs of Strongylocentrotus purpuratus by gene-specific hybridization and template activity.
1979,
Pubmed
,
Echinobase
Childs,
Orphons: dispersed genetic elements derived from tandem repetitive genes of eucaryotes.
1981,
Pubmed
,
Echinobase
Crain,
Actin gene expression in developing sea urchin embryos.
1981,
Pubmed
,
Echinobase
Denhardt,
A membrane-filter technique for the detection of complementary DNA.
1966,
Pubmed
Durica,
Organization of actin gene sequences in the sea urchin: molecular cloning of an intron-containing DNA sequence coding for a cytoplasmic actin.
1980,
Pubmed
,
Echinobase
Felsenfeld,
Methylation and gene control.
1982,
Pubmed
Grunstein,
Hatching in the sea urchin Lytechinus pictus is accompanied by a shift in histone H4 gene activity.
1978,
Pubmed
,
Echinobase
Hentschel,
The organization and expression of histone gene families.
1981,
Pubmed
Igo-Kemenes,
Chromatin.
1982,
Pubmed
Kedes,
Histone genes and histone messengers.
1979,
Pubmed
Kornberg,
The location of nucleosomes in chromatin: specific or statistical.
1981,
Pubmed
Levy,
Chromatin fine structure of active and repressed genes.
1981,
Pubmed
Lohr,
Detailed analysis of the nucleosomal organization of transcribed DNA in yeast chromatin.
1981,
Pubmed
Maniatis,
Nucleotide sequence of the rightward operator of phage lambda.
1975,
Pubmed
Maniatis,
Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.
1975,
Pubmed
Nedospasov,
Non-random cleavage of SV40 DNA in the compact minichromosome and free in solution by micrococcal nuclease.
1980,
Pubmed
Newrock,
Histone changes during chromatin remodeling in embryogenesis.
1978,
Pubmed
,
Echinobase
Newrock,
Stage-specific mRNAs coding for subtypes of H2A and H2B histones in the sea urchin embryo.
1978,
Pubmed
,
Echinobase
Noll,
Action of micrococcal nuclease on chromatin and the location of histone H1.
1977,
Pubmed
Palmiter,
Regulation of gene expression in the chick oviduct by steroid hormones.
1978,
Pubmed
Samal,
Chromatin structure of the histone genes of D. melanogaster.
1981,
Pubmed
Scheller,
Organization and expression of multiple actin genes in the sea urchin.
1981,
Pubmed
,
Echinobase
Spadafora,
The DNA repeat lengths in chromatins from sea urchin sperm and gastrule cells are markedly different.
1976,
Pubmed
,
Echinobase
Spinelli,
Evidences of two different sets of histone genes active during embryogenesis of the sea urchin Paracentrotus lividus.
1979,
Pubmed
,
Echinobase
Weisbrod,
Active chromatin.
1982,
Pubmed
Wu,
The chromatin structure of specific genes: II. Disruption of chromatin structure during gene activity.
1979,
Pubmed
Wu,
The 5' ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I.
1980,
Pubmed
Zachau,
Face to phase with nucleosomes.
1981,
Pubmed
