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J Cell Biol
1984 Oct 01;994 Pt 1:1251-8. doi: 10.1083/jcb.99.4.1251.
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Cytoplasmic dynein-like ATPase cross-links microtubules in an ATP-sensitive manner.
Hollenbeck PJ
,
Suprynowicz F
,
Cande WZ
.
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We have prepared dynein-like ATPase from the eggs of the sea urchin Strongylocentrotus purpuratus using differential centrifugation and column chromatography. This ATPase preparation is inhibited by vanadate and erythro-9-(3-[2-hydroxynonyl]) adenine (EHNA) at concentrations similar to those that inhibit reactivated flagellar beating and spindle elongation in lysed cell models. Using microtubule affinity and ATP-induced release, we can purify this ATPase activity to a composition on SDS PAGE of four peptides ranging in molecular weight from 180,000-300,000. When viewed in darkfield optics, this affinity-purified ATPase caused extensive parallel bundling of microtubule-associated protein-free microtubules. These bundles were dispersed by 1 mM ATP but not by ATP gamma S or AMP-5''-adenylimidodiphosphate. The reformation of microtubule bundles after dispersal by ATP required ATP hydrolysis; bundles did not reform in the presence of 10 microM vanadate. Negative stain electron microscopy of these bundled microtubules revealed that they are arranged in parallel networks with extensive close lateral association.
Beckerle,
Inhibitors of dynein activity block intracellular transport in erythrophores.
1982, Pubmed
Beckerle,
Inhibitors of dynein activity block intracellular transport in erythrophores.
1982,
Pubmed
Bloodgood,
Freeze-fracture of microtubules and bridges in motile axostyles.
1974,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Cande,
Nucleotide requirements for anaphase chromosome movements in permeabilized mitotic cells: anaphase B but not anaphase A requires ATP.
1982,
Pubmed
Clark,
Pigment particle translocation in detergent-permeabilized melanophores of Fundulus heteroclitus.
1982,
Pubmed
Forman,
Fast axonal transport in permeabilized lobster giant axons is inhibited by vanadate.
1983,
Pubmed
Forman,
Vanadate inhibits saltatory organelle movement in a permeabilized cell model.
1982,
Pubmed
Gibbons,
Functional recombination of dynein 1 with demembranated sea urchin sperm partially extracted with KC1.
1976,
Pubmed
,
Echinobase
Gibbons,
Potent inhibition of dynein adenosinetriphosphatase and of the motility of cilia and sperm flagella by vanadate.
1978,
Pubmed
,
Echinobase
Goodno,
Inhibition of myosin ATPase by vanadate ion.
1979,
Pubmed
Gratecos,
Adenosine 5'-O(3-thiotriphosphate) in the control of phosphorylase activity.
1974,
Pubmed
GRIMSTONE,
THE FINE STRUCTURE AND FUNCTION OF THE CONTRACTILE AXOSTYLES OF CERTAIN FLAGELLATES.
1965,
Pubmed
Haimo,
Dynein binds to and crossbridges cytoplasmic microtubules.
1979,
Pubmed
Haimo,
Dynein-microtubule interactions: ATP-sensitive dynein binding and the structural polarity of mitotic microtubules.
1982,
Pubmed
Hepler,
Intermicrotubule bridges in mitotic spindle apparatus.
1970,
Pubmed
Hisanaga,
Cytoplasmic dynein of the sea urchin egg. II. Purification, characterization and interactions with microtubules and Ca-calmodulin.
1983,
Pubmed
,
Echinobase
Kobayashi,
Inhibition of dynein ATPase by vanadate, and its possible use as a probe for the role of dynein in cytoplasmic motility.
1978,
Pubmed
,
Echinobase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Langford,
Length and appearance of projections on neuronal microtubules in vitro after negative staining: evidence against a crosslinking function for MAPs.
1983,
Pubmed
MAZIA,
Adenosine triphosphatase in the mitotic apparatus.
1961,
Pubmed
McDonald,
Cross-sectional structure of the central mitotic spindle of Diatoma vulgare. Evidence for specific interactions between antiparallel microtubules.
1979,
Pubmed
McDonald,
On the mechanism of anaphase spindle elongation in Diatoma vulgare.
1977,
Pubmed
Mitchell,
Interactions of dynein arms with b subfibers of Tetrahymena cilia: quantitation of the effects of magnesium and adenosine triphosphate.
1980,
Pubmed
Murofushi,
Bundling of microtubules in vitro by a high molecular weight protein prepared from the squid axon.
1983,
Pubmed
Murphy,
Association of high-molecular-weight proteins with microtubules and their role in microtubule assembly in vitro.
1975,
Pubmed
Pallini,
Cytoplasmic nucleoside-triphosphatase similar to axonemal dynein occur widely in different cell types.
1983,
Pubmed
Penningroth,
Dynein ATPase is inhibited selectively in vitro by erythro-9-[3-2-(hydroxynonyl)]adenine.
1982,
Pubmed
,
Echinobase
Penningroth,
Mechanochemical coupling in the relaxation of rigor-wave sea urchin sperm flagella.
1982,
Pubmed
,
Echinobase
Pratt,
The identification of a dynein ATPase in unfertilized sea urchin eggs.
1980,
Pubmed
,
Echinobase
Pratt,
Dynein-like Mg2+-ATPase in mitotic spindles isolated from sea urchin embryos (Strongylocentrotus droebachiensis).
1980,
Pubmed
,
Echinobase
Sakai,
The isolated mitotic apparatus and chromosome motion.
1978,
Pubmed
,
Echinobase
Sale,
Study of the mechanism of vanadate inhibition of the dynein cross-bridge cycle in sea urchin sperm flagella.
1979,
Pubmed
,
Echinobase
Satir,
The mechanochemical cycle of the dynein arm.
1981,
Pubmed
Shelanski,
Microtubule assembly in the absence of added nucleotides.
1973,
Pubmed
Shimizu,
Presteady state kinetic analysis of vanadate-induced inhibition of the dynein ATPase.
1983,
Pubmed
Shimizu,
Steady-state kinetic study of vanadate-induced inhibition of ciliary dynein adenosinetriphosphatase activity from Tetrahymena.
1981,
Pubmed
Summers,
Effects of trypsin digestion on flagellar structures and their relationship to motility.
1973,
Pubmed
,
Echinobase
Takahashi,
Binding of 30s dynein with the B-tubule of the outer doublet of axonemes from Tetrahymena pyriformis and adenosine triphosphate-induced dissociation of the complex.
1978,
Pubmed
Tucker,
Fine structure and function of the cytopharyngeal basket in the ciliate Nassula.
1968,
Pubmed
Warner,
Dynein: the mechanochemical coupling adenosine triphosphatase of microtubule-based sliding filament mechanisms.
1980,
Pubmed
Warner,
Polarity of dynein-microtubule interactions in vitro: cross-bridging between parallel and antiparallel microtubules.
1981,
Pubmed
Zanetti,
Effects of divalent cations on dynein cross bridging and ciliary microtubule sliding.
1979,
Pubmed
