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ECB-ART-34406
FEBS Lett 1984 Oct 01;1752:419-21. doi: 10.1016/0014-5793(84)80780-2.
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NAD+ kinase: molecular weight determination by low-angle laser-light scattering.

Gabriel MK , McGuinness ET .


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Low-angle laser-light scattering (LALLS) was employed to measure the absolute molecular weight of chicken liver NAD+ kinase (NADK). The weight-average molecular weight (Mw) was found to be 275 000 +/- 15 000. The corresponding value for the second virial coefficient was -1.65 X 10(-3) ml X mol X g2. The value for Mw is in close accord with estimates reported for pigeon liver (270 000) and C. utilis (260 000) NADK. If the active enzyme is a dimer, the weight difference between pigeon/chicken liver and rabbit liver (136 000) NADK would indicate that the latter enzyme is an active monomer unit.

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Genes referenced: nadk