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J Cell Biol
1971 Feb 01;482:324-39. doi: 10.1083/jcb.48.2.324.
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Selective extraction of isolated mitotic apparatus. Evidence that typical microtubule protein is extracted by organic mercurial.
Bibring T
,
Baxandall J
.
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Mitotic apparatus isolated from sea urchin eggs has been treated with meralluride sodium under conditions otherwise resembling those of its isolation. The treatment causes a selective morphological disappearance of microtubules while extracting a major protein fraction, probably consisting of two closely related proteins, which constitutes about 10% of mitotic apparatus protein. Extraction of other cell particulates under similar conditions yields much less of this protein. The extracted protein closely resembles outer doublet microtubule protein from sea urchin sperm tail in properties considered typical of microtubule proteins: precipitation by calcium ion and vinblastine, electrophoretic mobility in both acid and basic polyacrylamide gels, sedimentation coefficient, molecular weight, and, according to a preliminary determination, amino acid composition. An antiserum against a preparation of sperm tail outer doublet microtubules cross-reacts with the extract from mitotic apparatus. On the basis of these findings it appears that microtubule protein is selectively extracted from isolated mitotic apparatus by treatment with meralluride, and is a typical microtubule protein.
Adelman,
Cytoplasmic filaments and tubules.
1968, Pubmed
Adelman,
Cytoplasmic filaments and tubules.
1968,
Pubmed
Auclair,
Cilia regeneration in the sea urchin embryo: evidence for a pool of ciliary proteins.
1966,
Pubmed
,
Echinobase
BIBRING,
PERCENTAGE INCORPORATION OF LEUCINE LABELLED WITH CARBON-14 INTO ISOLATED MITOTIC APPARATUS DURING EARLY DEVELOPMENT OF SEA URCHIN EGGS.
1964,
Pubmed
,
Echinobase
Bensch,
Microtubular crystals in mammalian cells.
1969,
Pubmed
Bensch,
Induction in vitro of microtubular crystals by vinca alkaloids.
1969,
Pubmed
Bibring,
Mitotic apparatus: the selective extraction of protein with mild acid.
1968,
Pubmed
Bibring,
Immunochemical studies of 22S protein from isolated mitotic apparatus.
1969,
Pubmed
,
Echinobase
Borisy,
The mechanism of action of colchicine. Binding of colchincine-3H to cellular protein.
1967,
Pubmed
Borisy,
The mechanism of action of colchicine. Colchicine binding to sea urchin eggs and the mitotic apparatus.
1967,
Pubmed
,
Echinobase
Cohen,
An estimate of the amount of microtubule protein in the isolated mitotic apparatus.
1970,
Pubmed
DAVIS,
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.
1964,
Pubmed
DIRKSEN,
THE ISOLATION AND CHARACTERIZATION OF ASTERS FROM ARTIFICIALLY ACTIVATED SEA URCHIN EGGS.
1964,
Pubmed
,
Echinobase
Dunker,
Observations on molecular weight determinations on polyacrylamide gel.
1969,
Pubmed
Dutton,
Microtubular protein: synthesis and metabolism in developing brain.
1969,
Pubmed
Forer,
Comparisons of isolated and in vivo mitotic apparatuses.
1969,
Pubmed
Gibbons,
Chemical dissection of cilia.
1965,
Pubmed
Gibbons,
Dynein: A Protein with Adenosine Triphosphatase Activity from Cilia.
1965,
Pubmed
Goldman,
The structure and some properties of the isolated mitotic apparatus.
1969,
Pubmed
Hepler,
Intermicrotubule bridges in mitotic spindle apparatus.
1970,
Pubmed
Kane,
The mitotic apparatus. Identification of the major soluble component of the glycol-isolated mitotic apparatus.
1967,
Pubmed
,
Echinobase
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
LUFT,
Improvements in epoxy resin embedding methods.
1961,
Pubmed
Lindberg,
Molecular weight and amino acid composition of deoxyribonuclease I.
1967,
Pubmed
Marantz,
Vinblastine-induced precipitation of microtubule protein.
1969,
Pubmed
Marantz,
Structure of microtubular crystals induced by vinblastine in vitro.
1970,
Pubmed
Mazia,
The Isolation and Biochemical Characterization of the Mitotic Apparatus of Dividing Cells.
1952,
Pubmed
Mohri,
Amino-acid composition of "Tubulin" constituting microtubules of sperm flagella.
1968,
Pubmed
Moore,
Amino acid analysis: aqueous dimethyl sulfoxide as solvent for the ninhydrin reaction.
1968,
Pubmed
ORNSTEIN,
DISC ELECTROPHORESIS. I. BACKGROUND AND THEORY.
1964,
Pubmed
Olmsted,
Isolation of microtubule protein from cultured mouse neuroblastoma cells.
1970,
Pubmed
Puszkin,
Actin-like properties of colchicine binding protein isolated from brain.
1970,
Pubmed
Sakai,
Studies on sulfhydryl groups during cell division of sea-urchin eggs. 8. Some properties of mitotic apparatus proteins.
1966,
Pubmed
,
Echinobase
Shapiro,
Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels.
1967,
Pubmed
Shelanski,
Isolation of a protein subunit from microtubules.
1967,
Pubmed
,
Echinobase
Shelanski,
Properties of the protein subunit of central-pair and outer-doublet microtubules of sea urchin flagella.
1968,
Pubmed
,
Echinobase
Stephens,
The mitotic apparatus. Physical chemical characterization of the 22S protein component and its subunits.
1967,
Pubmed
,
Echinobase
Stephens,
Comparison of muscle actin and ciliary microtubule protein in the mollusk Pecten irradians.
1969,
Pubmed
Stephens,
Reassociation of microtubule protein.
1968,
Pubmed
Stephens,
Thermal fractionation of outer fiber doublet microtubules into A- and B-subfiber components. A- and B-tubulin.
1970,
Pubmed
Stephens,
Guanine nucleotide associated with the protein of the outer fibers of flagella and cilia.
1967,
Pubmed
,
Echinobase
Stephens,
On the structural protein of flagellar outer fibers.
1968,
Pubmed
VENABLE,
A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY.
1965,
Pubmed
Weber,
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
1969,
Pubmed
Weisenberg,
The colchicine-binding protein of mammalian brain and its relation to microtubules.
1968,
Pubmed
Wilson,
Arms and bridges on microtubules in the mitotic apparatus.
1969,
Pubmed
ZIMMERMAN,
Physico-chemical analysis of the isolated mitotic apparatus.
1960,
Pubmed
Zahler,
Removal of structural protein from mitochondria.
1968,
Pubmed
