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Chromosoma
1985 Jan 01;915:377-90. doi: 10.1007/bf00291012.
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The layered organization of nucleosomes in 30 nm chromatin fibers.
Subirana JA
,
Muñoz-Guerra S
,
Aymamí J
,
Radermacher M
,
Frank J
.
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We have used electron microscopy and established methods of three-dimensional reconstruction to obtain structural information on the 30 nm chromatin fibers from sea cucumber sperm and chicken erythrocytes. The fibers show a longitudinal periodicity of 10-11 nm. We have interpreted this periodicity as due to a grouping of nucleosomes into disks, each disk containing about 5-6 nucleosomes. These disks are closely stacked to form the chromatin fiber. We have built a detailed model for four fibers and we have determined the approximate coordinates of all the nucleosomes in them. The average distance found between neighboring nucleosomes has a value close to 11 nm. They may be connected either as a regularly distorted helix or as a layered zigzag. The second model appears more appropriate, since in the constrictions of the fibers the nucleosomes can only be connected as a zigzag.
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,
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Bates,
Stability of the higher-order structure of chicken-erythrocyte chromatin in solution.
1981,
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Burgoyne,
Avian erythrocyte chromatin degradation: the progressive exposure of the dinucleosomal repeat by bovine-pancreatic-DNAase-I-armed probes and free DNAase-I.
1982,
Pubmed
Butler,
Changes in chromatin folding in solution.
1980,
Pubmed
Cornudella,
Nucleosome organization during germ cell development in the sea cucumber Holothuria tubulosa.
1979,
Pubmed
,
Echinobase
Derenzini,
Visualization of a repeating subunit organization in rat hepatocyte chromatin fixed in situ.
1983,
Pubmed
Finch,
Solenoidal model for superstructure in chromatin.
1976,
Pubmed
Finch,
Structure of nucleosome core particles of chromatin.
1977,
Pubmed
Gilbert,
The reconstruction of a three-dimensional structure from projections and its application to electron microscopy. II. Direct methods.
1972,
Pubmed
Gordon,
Algebraic reconstruction techniques (ART) for three-dimensional electron microscopy and x-ray photography.
1970,
Pubmed
Houssier,
The structural organization of dinucleosomes and oligonucleosomes. Electric dichroism and birefringence study.
1981,
Pubmed
Igo-Kemenes,
Chromatin.
1982,
Pubmed
Itkes,
Repeating oligonucleosomal units. A new element of chromatin structure.
1980,
Pubmed
Marion,
The structural organization of oligonucleosomes.
1984,
Pubmed
Marion,
Conformation of chromatin oligomers. A new argument for a change with the hexanucleosome.
1981,
Pubmed
McGhee,
Higher order structure of chromatin: orientation of nucleosomes within the 30 nm chromatin solenoid is independent of species and spacer length.
1983,
Pubmed
,
Echinobase
McGhee,
Orientation of the nucleosome within the higher order structure of chromatin.
1980,
Pubmed
Olins,
Visualization of nucleosomes in thin sections by stereo electron microscopy.
1980,
Pubmed
Olins,
Electron microscope tomography: transcription in three dimensions.
1983,
Pubmed
Olins,
Spheroid chromatin units (v bodies).
1974,
Pubmed
Olins,
nu bodies are close-packed in chromatin fibers.
1978,
Pubmed
Olins,
Ultrastructural features of chromatin nu bodies.
1976,
Pubmed
Osipova,
The role of histone H1 in compaction of nucleosomes. Sedimentation behaviour of oligonucleosomes in solution.
1980,
Pubmed
Rattner,
Higher order structure in metaphase chromosomes. I. The 250 A fiber.
1978,
Pubmed
Rattner,
Higher order structure in metaphase chromosomes. II. The relationship between the 250 A fiber, superbeads and beads-on-a-string.
1978,
Pubmed
Renz,
Involvement of histone H1 in the organization of the chromosome fiber.
1977,
Pubmed
Ruiz-Carrillo,
Stability and reversibility of higher ordered structure of interphase chromatin: continuity of deoxyribonucleic acid is not required for maintenance of folded structure.
1980,
Pubmed
Simpson,
Structure of the chromatosome, a chromatin particle containing 160 base pairs of DNA and all the histones.
1978,
Pubmed
Staynov,
Nuclease digestion patterns as a criterion for nucleosome orientation in the higher order structure of chromatin.
1983,
Pubmed
Strogatz,
Topology of zigzag chromatin.
1983,
Pubmed
Subirana,
The subunit structure of chromatin fibres.
1981,
Pubmed
,
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Subirana,
Three-dimensional reconstruction of chromatin fibers.
1983,
Pubmed
Thoma,
Involvement of histone H1 in the organization of the nucleosome and of the salt-dependent superstructures of chromatin.
1979,
Pubmed
Thomas,
Cross-linking of histone H1 in chromatin.
1980,
Pubmed
Thomas,
Size-dependence of a stable higher-order structure of chromatin.
1980,
Pubmed
Woodcock,
Ultrastructure of chromatin subunits during unfolding, histone depletion, and reconstitution.
1978,
Pubmed
Worcel,
Structure of chromatin and the linking number of DNA.
1981,
Pubmed
Zentgraf,
Differences of supranucleosomal organization in different kinds of chromatin: cell type-specific globular subunits containing different numbers of nucleosomes.
1984,
Pubmed
,
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