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Echinobase
ECB-ART-32972
Experientia 1985 Aug 15;418:1068-70. doi: 10.1007/bf01952148.
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Injection of a soluble sperm fraction into sea-urchin eggs triggers the cortical reaction.

Dale B , DeFelice LJ , Ehrenstein G .


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Fertilization membranes form around unfertilized sea-urchin eggs after microinjection of a soluble spermatozoa fraction isosmotic with seawater. This demonstrates that the spermatozoon contains a chemical that triggers an increase in cytosolic calcium, leading to exocytosis of cortical granules. It also demonstrates that the triggering mechanism does not require an externally-activated egg-membrane process. Further experiments show that the chemical trigger is not calcium.

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Genes referenced: LOC100887844 LOC593358

References [+] :
Hamaguchi, Activation of sea urchin eggs by microinjection of calcium buffers. 1981, Pubmed, Echinobase