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J Muscle Res Cell Motil
1985 Aug 01;64:507-12. doi: 10.1007/bf00712586.
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Resolution of the competitive inhibitory effects of lithium and AMPPNP on the beat frequency of ATP-reactivated, demembranated, sea urchin sperm flagella.
Pate EF
,
Brokaw CJ
.
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About half of the inhibitory effect on the beat frequency of sea urchin spermatozoa flagella by beta, gamma-imido-adenosine-5''-triphosphate (AMPPNP) previously reported by Okuno & Brokaw (1981) was actually the result of inhibition by lithium resulting from the use of a tetralithium salt of AMPPNP. Li+ (or LiATP3-) acts as a competitive inhibitor of beat frequency, with a Ki for Li+ of about 30 mM. Under improved reactivation conditions which reduce the Km for beat frequency to about 0.1 mM, the Ki for lithium-free MgAMPPNP2- is about 2.3 mM, and magnesium pyrophosphate (MgPPi-), which may be an equivalent ligand, gives a Ki of 3.4 mM.
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Properties of flagellar "rigor waves" formed by abrupt removal of adenosine triphosphate from actively swimming sea urchin sperm.
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The pathway of ATP hydrolysis by dynein. Kinetics of a presteady state phosphate burst.
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Inhibition of movement of trition-demembranated sea-urchin sperm flagella by Mg2+, ATP4-, ADP and P1.
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Okuno,
Effects of AMPPNP and vanadate on the mechanochemical crossbridge cycle in flagella.
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Pate,
The inhibition of muscle contraction by adenosine 5' (beta, gamma-imido) triphosphate and by pyrophosphate.
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Effects of adenylyl imidodiphosphate, a nonhydrolyzable adenosine triphosphate analog, on reactivated and rigor wave sea urchin sperm.
1978,
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Porter,
Transient state kinetic analysis of the ATP-induced dissociation of the dynein-microtubule complex.
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Binding of 30s dynein with the B-tubule of the outer doublet of axonemes from Tetrahymena pyriformis and adenosine triphosphate-induced dissociation of the complex.
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Takahashi,
Kinetic properties of dynein ATPase from Tetrahymena pyriformis. The initial phosphate burst of dynein ATPase and its interaction with ATP analogs.
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Yount,
Adenylyl imidodiphosphate, an adenosine triphosphate analog containing a P--N--P linkage.
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