Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
J Cell Biol
1986 Nov 01;1035:1911-9. doi: 10.1083/jcb.103.5.1911.
Show Gene links
Show Anatomy links
Purification and characterization of a 190-kD microtubule-associated protein from bovine adrenal cortex.
Murofushi H
,
Kotani S
,
Aizawa H
,
Hisanaga S
,
Hirokawa N
,
Sakai H
.
???displayArticle.abstract???
A heat-stable microtubule-associated protein (MAP) with molecular weight of 190,000, termed 190-kD MAP, was purified from bovine adrenal cortex. This MAP showed the same level of ability to promote tubulin polymerization as did MAP2 and tau from mammalian brains. Relatively high amounts of 190-kD MAP could bind to microtubules reconstituted in the presence of taxol. At maximum 1 mol of 190-kD MAP could bind to 2.3 mol of tubulin. 190-kD MAP was phosphorylated by a cAMP-dependent protein kinase prepared from sea urchin spermatozoa and by protein kinase(s) present in the microtubule protein fraction prepared from mammalian brains. The maximal numbers of incorporated phosphate were approximately 0.2 and approximately 0.4 mol per mole of 190-kD MAP, respectively. These values were lower than that of MAP2, which could be heavily phosphorylated by the endogenous protein kinase(s) up to 5 mol per mole of MAP2 under the same assay condition. 190-kD MAP had no effects on the low-shear viscosity of actin and did not induce an increase in turbidity of the actin solution. It was also revealed that 190-kD MAP does not cosediment with actin filaments. These data clearly show that, distinct from MAP2 and tau, this MAP does not interact with actin. Electron microscopic observation of the rotary-shadowed images of 190-kD MAP showed the molecular shape to be a long, thin, flexible rod with a contour length of approximately 100 nm. Quick-freeze, deep-etch replicas of the microtubules reconstituted from 190-kD MAP and brain tubulin revealed many cross-bridges connecting microtubules with each other.
Bader,
Immunocytochemical study of microtubules in chromaffin cells in culture and evidence that tubulin is not an integral protein of the chromaffin granule membrane.
1981, Pubmed
Bader,
Immunocytochemical study of microtubules in chromaffin cells in culture and evidence that tubulin is not an integral protein of the chromaffin granule membrane.
1981,
Pubmed
Berk,
Thyroid and brain microtubules: a comparison.
1980,
Pubmed
Berk,
Purification of a stimulator of microtubule assembly from bovine thyroid.
1981,
Pubmed
Binder,
The distribution of tau in the mammalian central nervous system.
1985,
Pubmed
Bloom,
Widespread distribution of the major polypeptide component of MAP 1 (microtubule-associated protein 1) in the nervous system.
1984,
Pubmed
Bloom,
Identification of high molecular weight microtubule-associated proteins in anterior pituitary tissue and cells using taxol-dependent purification combined with microtubule-associated protein specific antibodies.
1985,
Pubmed
Bulinski,
Widespread distribution of a 210,000 mol wt microtubule-associated protein in cells and tissues of primates.
1980,
Pubmed
Bulinski,
Microtubule-associated proteins from cultured HeLa cells. Analysis of molecular properties and effects on microtubule polymerization.
1980,
Pubmed
Burns,
The multiple phosphorylation of the microtubule-associated protein MAP2 controls the MAP2:tubulin interaction.
1984,
Pubmed
Cleveland,
Purification of tau, a microtubule-associated protein that induces assembly of microtubules from purified tubulin.
1977,
Pubmed
Cleveland,
Conservation of microtubule associated proteins. Isolation and characterization of tau and the high molecular weight microtubule associated protein from chicken brain and from mouse fibroblasts and comparison to the corresponding mammalian brain proteins.
1979,
Pubmed
Doenges,
Assembly of nonneural microtubules in the absence of glycerol and microtubule-associated proteins.
1979,
Pubmed
Drubin,
Nerve growth factor-induced neurite outgrowth in PC12 cells involves the coordinate induction of microtubule assembly and assembly-promoting factors.
1985,
Pubmed
Gottlieb,
Analysis of the microtubule-binding domain of MAP-2.
1985,
Pubmed
Greene,
Regulation of a high molecular weight microtubule-associated protein in PC12 cells by nerve growth factor.
1983,
Pubmed
Griffith,
Evidence for actin filament-microtubule interaction mediated by microtubule-associated proteins.
1978,
Pubmed
Gubler,
Molecular cloning establishes proenkephalin as precursor of enkephalin-containing peptides.
1982,
Pubmed
Heidemann,
Synergistic effects of cyclic AMP and nerve growth factor on neurite outgrowth and microtubule stability of PC12 cells.
1985,
Pubmed
Herzog,
Fractionation of brain microtubule-associated proteins. Isolation of two different proteins which stimulate tubulin polymerization in vitro.
1978,
Pubmed
Hirokawa,
Quick-freeze, deep-etch visualization of the cytoskeleton beneath surface differentiations of intestinal epithelial cells.
1981,
Pubmed
Hirokawa,
Cytoskeletal architecture and immunocytochemical localization of microtubule-associated proteins in regions of axons associated with rapid axonal transport: the beta,beta'-iminodipropionitrile-intoxicated axon as a model system.
1985,
Pubmed
Hirokawa,
270K microtubule-associated protein cross-reacting with anti-MAP2 IgG in the crayfish peripheral nerve axon.
1986,
Pubmed
Hirokawa,
Cross-linker system between neurofilaments, microtubules, and membranous organelles in frog axons revealed by the quick-freeze, deep-etching method.
1982,
Pubmed
Huber,
MAP3: characterization of a novel microtubule-associated protein.
1985,
Pubmed
Ishiguro,
Evidence that cAMP-dependent protein kinase and a protein factor are involved in reactivation of triton X-100 models of sea urchin and starfish spermatozoa.
1982,
Pubmed
,
Echinobase
Kim,
The periodic association of MAP2 with brain microtubules in vitro.
1979,
Pubmed
Klein,
A high molecular weight phosphoprotein in cultured fibroblasts that associates with polymerized tubulin.
1978,
Pubmed
Kotani,
33K protein--an inhibitory factor of tubulin polymerization in porcine brain.
1984,
Pubmed
,
Echinobase
Kotani,
Characterization of microtubule-associated proteins isolated from bovine adrenal gland.
1986,
Pubmed
Kotani,
Calmodulin inhibits interaction of actin with MAP2 and Tau, two major microtubule-associated proteins.
1985,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Livett,
Use of isolated chromaffin cells to study basic release mechanisms.
1983,
Pubmed
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
MacLean-Fletcher,
Viscometric analysis of the gelation of Acanthamoeba extracts and purification of two gelation factors.
1980,
Pubmed
Magalhães,
Effects of vinblastine and colchicine on the rat adrenal cortex: morphometric and cytochemical studies.
1985,
Pubmed
Matus,
High molecular weight microtubule-associated proteins are preferentially associated with dendritic microtubules in brain.
1981,
Pubmed
Murofushi,
Bundling of microtubules in vitro by a high molecular weight protein prepared from the squid axon.
1983,
Pubmed
Murphy,
Identity and polymerization-stimulatory activity of the nontubulin proteins associated with microtubules.
1977,
Pubmed
Nagle,
Assembly of tubulin from cultured cells and comparison with the neurotubulin model.
1977,
Pubmed
Noda,
Cloning and sequence analysis of cDNA for bovine adrenal preproenkephalin.
1982,
Pubmed
Olmsted,
A microtubule-associated protein specific to differentiated neuroblastoma cells.
1981,
Pubmed
Parysek,
MAP 4: a microtubule-associated protein specific for a subset of tissue microtubules.
1984,
Pubmed
Parysek,
MAP 4: occurrence in mouse tissues.
1984,
Pubmed
Peng,
Biochemical and immunological analyses of cytoskeletal domains of neurons.
1986,
Pubmed
Sato,
Effect upon mitogenic stimulation of calcium-dependent phosphorylation of cytoskeleton-associated 350,000- and 80,000-mol-wt polypeptides in quiescent 3Y1 cells.
1985,
Pubmed
Shelanski,
Evidence for interactions between neurofilaments and microtubules.
1981,
Pubmed
Shelanski,
Microtubule assembly in the absence of added nucleotides.
1973,
Pubmed
Sloboda,
Cyclic AMP-dependent endogenous phosphorylation of a microtubule-associated protein.
1975,
Pubmed
Sloboda,
Microtubule-associated proteins and the stimulation of tubulin assembly in vitro.
1976,
Pubmed
Solomon,
Identification with cellular microtubules of one of the co-assemlbing microtubule-associated proteins.
1979,
Pubmed
Spudich,
The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.
1971,
Pubmed
Suprenant,
Association between endocrine pancreatic secretory granules and in-vitro-assembled microtubules is dependent upon microtubule-associated proteins.
1982,
Pubmed
Theurkauf,
Molecular characterization of the cAMP-dependent protein kinase bound to microtubule-associated protein 2.
1982,
Pubmed
Tyler,
Rotary shadowing of extended molecules dried from glycerol.
1980,
Pubmed
Vallee,
A taxol-dependent procedure for the isolation of microtubules and microtubule-associated proteins (MAPs).
1982,
Pubmed
Vallee,
Structure and phosphorylation of microtubule-associated protein 2 (MAP 2).
1980,
Pubmed
Vallee,
A protein kinase bound to the projection portion of MAP 2 (microtubule-associated protein 2).
1981,
Pubmed
Voter,
Electron microscopy of MAP 2 (microtubule-associated protein 2).
1982,
Pubmed
Weatherbee,
Purification and reconstitution of HeLa cell microtubules.
1980,
Pubmed
Weatherbee,
Microtubule-associated proteins of HeLa cells: heat stability of the 200,000 mol wt HeLa MAPs and detection of the presence of MAP-2 in HeLa cell extracts and cycled microtubules.
1982,
Pubmed
Weber,
The isolation of tubulin and actin from mouse 3T3 cells transformed by simian virus 40 (SV3T3 cells), an established cell line growing in culture.
1977,
Pubmed
Weingarten,
A protein factor essential for microtubule assembly.
1975,
Pubmed
Wiche,
Widespread occurrence of polypeptides related to neurotubule-associated proteins (MAP-1 and MAP-2) in non-neuronal cells and tissues.
1984,
Pubmed
Wiche,
Microtubule protein preparations from C6 glial cells and their spontaneous polymer formation.
1979,
Pubmed
