Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
???displayArticle.abstract???
In unfertilized eggs of the sea urchin, Strongylocentrotus purpuratus, glucose-6-phosphate dehydrogenase (G6PDH) associates with the particulate elements remaining either after homogenization or extraction of eggs with non-ionic detergent in low ionic-strength media. At physiological ionic strength, the extent of G6PDH binding to these particulate elements is proportional to the total protein concentration in the extracts. In fertilized eggs this association is prevented by one or more low molecular weight solutes. The dissociation is reversible, and there are no permanent modifications of either G6PDH or its particulate binding site that affect binding. After fertilization, the time course of dissociation of G6PDH from particulate elements is too fast to be caused by a change in intracellular pH, but it could be triggered, but not maintained, by an increase in the intracellular calcium concentration. Binding of G6PDH to the particulate fraction lowers its catalytic activity at all substrate concentrations. Therefore, release of the enzyme into the cytoplasm may be an important part of the suite of events causing metabolic activation of the egg at fertilization.
Barber,
Mechanisms of G6PD isozyme pattern changes at fertilization.
1982, Pubmed,
Echinobase
Barber,
Mechanisms of G6PD isozyme pattern changes at fertilization.
1982,
Pubmed
,
Echinobase Clegg,
Properties and metabolism of the aqueous cytoplasm and its boundaries.
1984,
Pubmed Epel,
Mechanisms of activation of sperm and egg during fertilization of sea urchin gametes.
1978,
Pubmed
,
Echinobase Esen,
A simple method for quantitative, semiquantitative, and qualitative assay of protein.
1978,
Pubmed Fulton,
How crowded is the cytoplasm?
1982,
Pubmed Helmerhorst,
Microcentrifuge desalting: a rapid, quantitative method for desalting small amounts of protein.
1980,
Pubmed Ii,
Release of glucose-6-phosphate dehydrogenase from cortex of Spisula eggs at fertilization and its recombination after meiosis.
1982,
Pubmed Isono,
Pathways of carbohydrate breakdown in sea urchin eggs.
1968,
Pubmed
,
Echinobase Johnson,
Intracellular pH of sea urchin eggs measured by the dimethyloxazolidinedione (DMO) method.
1981,
Pubmed
,
Echinobase Kliman,
Association of glyceraldehyde-3-phosphate dehydrogenase with the human red cell membrane. A kinetic analysis.
1980,
Pubmed KRAHL,
Oxidative pathways for glucose in eggs of the sea urchin.
1956,
Pubmed
,
Echinobase Levy,
Glucose-6-phosphate dehydrogenases.
1979,
Pubmed Liu,
Hemoglobin enhances the self-association of spectrin heterodimers in human erythrocytes.
1984,
Pubmed Masters,
Interactions between soluble enzymes and subcellular structure.
1981,
Pubmed Minton,
The effect of volume occupancy upon the thermodynamic activity of proteins: some biochemical consequences.
1983,
Pubmed Mirande,
Association of an aminoacyl-tRNA synthetase complex and of phenylalanyl-tRNA synthetase with the cytoskeletal framework fraction from mammalian cells.
1985,
Pubmed Poenie,
Changes of free calcium levels with stages of the cell division cycle.
1985,
Pubmed
,
Echinobase Schmidt,
High hydrostatic pressure and the dissection of fertilization responses. I. The relationship between cortical granule exocytosis and proton efflux during fertilization of the sea urchin egg.
1983,
Pubmed
,
Echinobase Shen,
Intracellular pH and the sodium requirement at fertilisation.
1979,
Pubmed
,
Echinobase Strapazon,
Interaction of the aldolase and the membrane of human erythrocytes.
1977,
Pubmed Suprynowicz,
Fluctuation of the Ca-sequestering activity of permeabilized sea urchin embryos during the cell cycle.
1985,
Pubmed
,
Echinobase Van Noorden,
Histochemistry and cytochemistry of glucose-6-phosphate dehydrogenase.
1984,
Pubmed Whitaker,
The relation between the increase in reduced nicotinamide nucleotides and the initiation of DNA synthesis in sea urchin eggs.
1981,
Pubmed
,
Echinobase Whitaker,
Ionic regulation of egg activation.
1982,
Pubmed
,
Echinobase
