Exp Cell Res 1986 Aug 01;1652:429-40. doi: 10.1016/0014-4827(86)90596-3.

Nuclear AP4A-binding activity of sea urchin embryos changes in relation to the initiation of S phase.

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The AP4A-binding activity of sea urchin embryos was studied using radioactively labelled diadenosine 5'',5"''-P1,P4-tetraphosphate (Ap4A). Among various subcellular components that can bind [3H]AP4A, nuclei alone showed the highly specific Ap4A-binding activity which was not influenced by the presence of AP4A, AP5A and GP4G. The addition of an excess amount of ATP only slightly reduced the binding of [3H]AP4A to the nuclei. It was found that AP4A binds to the residual proteinaceous structure of nuclei which was resistant to the extraction with 2 M NaCl. The nuclear AP4A-binding activity fluctuated cyclically during each cell cycle, with a transient increase at the beginning of S phase followed by an abrupt decrease within 10 min. When the initiation of S phase was blocked, the increase in the AP4A-binding activity was also prevented. It seems that the binding of AP4A to the nuclear structural protein is involved in the initiation of S phase.

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Genes referenced: LOC100887844 LOC115919910