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J Membr Biol
1988 Mar 01;1013:199-207. doi: 10.1007/bf01872835.
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Ionic and permeability requirements for exocytosis in vitro in sea urchin eggs.
Zimmerberg J
,
Liu J
.
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We study exocytosis in the planar isolated cortex of the egg of the sea urchin Lytechinus pictus. Solutions bathing the exocytotic apparatus need not contain appreciable amounts of ions: fusion follows addition of submicromolar calcium to solutions containing only nonelectrolyte. We examine the effects of altering the granule membrane permeability to small molecules with ionophores and digitonin. Introducing holes in the secretory granule membrane to the extent of allowing free passage of small molecules does not cause secretion in vitro. We add the amphipathic compound digitonin at 12 to 15 microM concentrations and demonstrate that the granule membrane can become permeable to lucifer yellow, yet that granules remain intact. Granules still undergo exocytosis after digitonin treatment at such concentrations upon subsequent addition of calcium. Higher concentrations of digitonin lead to granule content swelling and vesicle bursting. We conclude that cortical granule hydration during exocytosis is not mediated by small ionic channels.
Akabas,
Separation of the osmotically driven fusion event from vesicle-planar membrane attachment in a model system for exocytosis.
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Akabas,
Separation of the osmotically driven fusion event from vesicle-planar membrane attachment in a model system for exocytosis.
1984,
Pubmed
Andersen,
Effect of phloretin on the permeability of thin lipid membranes.
1976,
Pubmed
Andreoli,
The effect of valinomycin on the ionic permeability of thin lipid membranes.
1967,
Pubmed
Baker,
Influence of ATP and calcium on the cortical reaction in sea urchin eggs.
1978,
Pubmed
,
Echinobase
Bilinski,
Secretory protein decondensation as a distinct, Ca2+-mediated event during the final steps of exocytosis in Paramecium cells.
1981,
Pubmed
Brown,
Role of anions in parathyroid hormone release from dispersed bovine parathyroid cells.
1978,
Pubmed
Cass,
Equilibrium dialysis of ions in nystatin-treated red cells.
1973,
Pubmed
Christen,
Acidic vesicles and the uptake of amines by sea urchin eggs.
1983,
Pubmed
,
Echinobase
Cohen,
Osmotic swelling of phospholipid vesicles causes them to fuse with a planar phospholipid bilayer membrane.
1982,
Pubmed
Cohen,
Fusion of phospholipid vesicles with planar phospholipid bilayer membranes. II. Incorporation of a vesicular membrane marker into the planar membrane.
1980,
Pubmed
Cohen,
Parameters affecting the fusion of unilamellar phospholipid vesicles with planar bilayer membranes.
1984,
Pubmed
Crabb,
In vitro reconstitution of exocytosis from plasma membrane and isolated secretory vesicles.
1985,
Pubmed
,
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Dunn,
Catecholamine secretion from digitonin-treated adrenal medullary chromaffin cells.
1983,
Pubmed
Ferris,
Effects of various agents on the Mg2+-ATP stimulated incorporation and release of catecholamines by isolated bovine adrenomedullary storage vesicles and on secretion from the adrenal medulla.
1970,
Pubmed
Finkelstein,
Osmotic swelling of vesicles: its role in the fusion of vesicles with planar phospholipid bilayer membranes and its possible role in exocytosis.
1986,
Pubmed
,
Echinobase
Green,
The course of the acrosome reaction in guinea-pig sperm.
1982,
Pubmed
Green,
The induction of the acrosome reaction in guinea-pig sperm by the divalent metal cation ionophore A23187.
1978,
Pubmed
Harafuji,
Re-examination of the apparent binding constant of ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid with calcium around neutral pH.
1980,
Pubmed
Holz,
Effects of osmolality and ionic strength on secretion from adrenal chromaffin cells permeabilized with digitonin.
1986,
Pubmed
Knight,
Calcium-dependence of catecholamine release from bovine adrenal medullary cells after exposure to intense electric fields.
1982,
Pubmed
Kopf,
Isolation and characterization of sea urchin egg cortical granules.
1982,
Pubmed
,
Echinobase
Lee,
Changes in intracellular acidic compartments in sea urchin eggs after activation.
1983,
Pubmed
,
Echinobase
Marty,
Ca-dependent K channels with large unitary conductance in chromaffin cell membranes.
1981,
Pubmed
Sardet,
A marker of animal-vegetal polarity in the egg of the sea urchin Paracentrotus lividus. The pigment band.
1985,
Pubmed
,
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Stanley,
A model for exocytosis based on the opening of calcium-activated potassium channels in vesicles.
1985,
Pubmed
Vacquier,
The isolation of intact cortical granules from sea urchin eggs: calcium lons trigger granule discharge.
1975,
Pubmed
,
Echinobase
Whitaker,
Inhibition of secretory granule discharge during exocytosis in sea urchin eggs by polymer solutions.
1987,
Pubmed
,
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Whitaker,
Calcium-dependent exocytosis in an in vitro secretory granule plasma membrane preparation from sea urchin eggs and the effects of some inhibitors of cytoskeletal function.
1983,
Pubmed
,
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Wilson,
Calcium-evoked secretion from digitonin-permeabilized adrenal medullary chromaffin cells.
1983,
Pubmed
Yellen,
Single Ca2+-activated nonselective cation channels in neuroblastoma.
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Zimmerberg,
Irreversible swelling of secretory granules during exocytosis caused by calcium.
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,
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Exocytosis of sea urchin egg cortical vesicles in vitro is retarded by hyperosmotic sucrose: kinetics of fusion monitored by quantitative light-scattering microscopy.
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,
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