Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Mol Cell Biol
1986 Nov 01;611:4008-18. doi: 10.1128/mcb.6.11.4008-4018.1986.
Show Gene links
Show Anatomy links
Tripartite sequences within and 3'' to the sea urchin H2A histone gene display properties associated with a transcriptional termination process.
Johnson MR
,
Norman C
,
Reeve MA
,
Scully J
,
Proudfoot NJ
.
???displayArticle.abstract???
We have defined a DNA sequence that behaves as an RNA polymerase II termination signal by using the human HeLa cell transient expression system. Surprisingly, this sequence is tripartite, including part of the coding region of the sea urchin H2A histone gene together with two separate sequences in the 3'' flanking region of the gene. We demonstrate that this signal functions both in its normal gene environment and also when placed within the human alpha-globin gene. However, we have failed to detect a discrete 3'' terminus. Rather, our data indicate the presence of an extremely heterogeneous series of nonpolyadenylated RNAs. These heterogeneous nonpolyadenylated RNAs are stable when transcribed from the intact histone gene but are highly unstable within the human alpha-globin gene. This provides evidence for the role of poly(A) in the stability of mRNA.
Bendig,
Transcription of sea urchin histone genes in HeLa cells.
1983, Pubmed,
Echinobase
Bendig,
Transcription of sea urchin histone genes in HeLa cells.
1983,
Pubmed
,
Echinobase
Birchmeier,
The terminal RNA stem-loop structure and 80 bp of spacer DNA are required for the formation of 3' termini of sea urchin H2A mRNA.
1983,
Pubmed
,
Echinobase
Birchmeier,
3' editing of mRNAs: sequence requirements and involvement of a 60-nucleotide RNA in maturation of histone mRNA precursors.
1984,
Pubmed
,
Echinobase
Birchmeier,
Generation of authentic 3' termini of an H2A mRNA in vivo is dependent on a short inverted DNA repeat and on spacer sequences.
1982,
Pubmed
,
Echinobase
Birnstiel,
Transcription termination and 3' processing: the end is in site!
1985,
Pubmed
Bogenhagen,
Nucleotide sequences in Xenopus 5S DNA required for transcription termination.
1981,
Pubmed
Citron,
Transcription termination occurs within a 1000 base pair region downstream from the poly(A) site of the mouse beta-globin (major) gene.
1984,
Pubmed
Conway,
A sequence downstream of A-A-U-A-A-A is required for formation of simian virus 40 late mRNA 3' termini in frog oocytes.
1985,
Pubmed
Falck-Pedersen,
Transcription termination within the E1A gene of adenovirus induced by insertion of the mouse beta-major globin terminator element.
1985,
Pubmed
Ford,
Transcription pattern of in vivo-labeled late simian virus 40 RNA: equimolar transcription beyond the mRNA 3' terminus.
1978,
Pubmed
Fraser,
The major late adenovirus type-2 transcription unit: termination is downstream from the last poly(A) site.
1979,
Pubmed
Galli,
Biochemical complementation with RNA in the Xenopus oocyte: a small RNA is required for the generation of 3' histone mRNA termini.
1983,
Pubmed
,
Echinobase
Gil,
A sequence downstream of AAUAAA is required for rabbit beta-globin mRNA 3'-end formation.
1985,
Pubmed
Grosschedl,
Spacer DNA sequences upstream of the T-A-T-A-A-A-T-A sequence are essential for promotion of H2A histone gene transcription in vivo.
1980,
Pubmed
,
Echinobase
Grosschedl,
Identification of regulatory sequences in the prelude sequences of an H2A histone gene by the study of specific deletion mutants in vivo.
1980,
Pubmed
,
Echinobase
Grosveld,
DNA sequences necessary for transcription of the rabbit beta-globin gene in vivo.
1982,
Pubmed
Groudine,
Transcriptional regulation of hemoglobin switching in chicken embryos.
1981,
Pubmed
Hagenbüchle,
Termination of transcription in the mouse alpha-amylase gene Amy-2a occurs at multiple sites downstream of the polyadenylation site.
1984,
Pubmed
Hart,
Poly(A) site cleavage in a HeLa nuclear extract is dependent on downstream sequences.
1985,
Pubmed
Henikoff,
Transcription terminates in yeast distal to a control sequence.
1983,
Pubmed
Hentschel,
The organization and expression of histone gene families.
1981,
Pubmed
Hentschel,
Homocopolymer sequences in the spacer of a sea urchin histone gene repeat are sensitive to S1 nuclease.
1982,
Pubmed
,
Echinobase
Hofer,
Globin RNA transcription: a possible termination site and demonstration of transcriptional control correlated with altered chromatin structure.
1982,
Pubmed
Krämer,
The 5' terminus of the RNA moiety of U1 small nuclear ribonucleoprotein particles is required for the splicing of messenger RNA precursors.
1984,
Pubmed
Krieg,
Formation of the 3' end of histone mRNA by post-transcriptional processing.
1984,
Pubmed
,
Echinobase
LeMeur,
Termination of the ovalbumin gene transcription.
1984,
Pubmed
Lerner,
Are snRNPs involved in splicing?
1980,
Pubmed
Manley,
Accurate and specific polyadenylation of mRNA precursors in a soluble whole-cell lysate.
1983,
Pubmed
Mather,
Mode of regulation of immunoglobulin mu- and delta-chain expression varies during B-lymphocyte maturation.
1984,
Pubmed
Maxam,
A new method for sequencing DNA.
1977,
Pubmed
McDevitt,
Requirement of a downstream sequence for generation of a poly(A) addition site.
1984,
Pubmed
McLauchlan,
The consensus sequence YGTGTTYY located downstream from the AATAAA signal is required for efficient formation of mRNA 3' termini.
1985,
Pubmed
Mellon,
Identification of DNA sequences required for transcription of the human alpha 1-globin gene in a new SV40 host-vector system.
1981,
Pubmed
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Moore,
Accurate cleavage and polyadenylation of exogenous RNA substrate.
1985,
Pubmed
Nevins,
Transcription units of adenovirus type 2. Termination of transcription beyond the poly(A) addition site in early regions 2 and 4.
1980,
Pubmed
Nevins,
Steps in the processing of Ad2 mRNA: poly(A)+ nuclear sequences are conserved and poly(A) addition precedes splicing.
1978,
Pubmed
Nevins,
The pathway of eukaryotic mRNA formation.
1983,
Pubmed
Nordstrom,
Polyadenylylation of sea urchin histone RNA sequences in transfected COS cells.
1985,
Pubmed
,
Echinobase
Padgett,
Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein.
1983,
Pubmed
Price,
The 3' end of drosophila histone H3 mRNA is produced by a processing activity in vitro.
1984,
Pubmed
Proudfoot,
The end of the message and beyond.
1984,
Pubmed
Proudfoot,
Transcriptional analysis of human zeta globin genes.
1984,
Pubmed
Pulleyblank,
A structural basis for S1 nuclease sensitivity of double-stranded DNA.
1985,
Pubmed
Rogers,
A mechanism for RNA splicing.
1980,
Pubmed
Rohrbaugh,
Transcription unit of the rabbit beta 1 globin gene.
1985,
Pubmed
Rosenberg,
Regulatory sequences involved in the promotion and termination of RNA transcription.
1979,
Pubmed
Sato,
A specific DNA sequence controls termination of transcription in the gastrin gene.
1986,
Pubmed
Sheffery,
Gene expression in murine erythroleukemia cells. Transcriptional control and chromatin structure of the alpha 1-globin gene.
1984,
Pubmed
Strub,
The cDNA sequences of the sea urchin U7 small nuclear RNA suggest specific contacts between histone mRNA precursor and U7 RNA during RNA processing.
1984,
Pubmed
,
Echinobase
Weintraub,
Alpha-Globin-gene switching during the development of chicken embryos: expression and chromosome structure.
1981,
Pubmed
