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Production of an antibody-like factor in the sea star Asterias rubens: involvement of at least three cellular populations.
Leclerc M
,
Brillouet C
,
Luquet G
,
Binaghi RA
.
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Cells from the axial organ of sea stars stimulated in vivo with TNP coupled to polyacrylamide beads and subsequently cultured in vitro were able to produce an antibody-like factor which induced the lysis of mammalian red cells sensitized with TNP. The axial organ cells were fractionated in two populations, adherent and non-adherent to nylon wool. The release of the antibody-like factor required the contact of both populations. When the adherent cells were disrupted by sonication the factor was not produced, but the non-adherent cells could be substituted by their membranes. Destruction by silica of the phagocytic cells present in the adherent population inhibited the production of the factor. The addition of mercaptoethanol to the cultures was essential and did not neutralize the effect of silica. It is concluded that at least three types of cells are involved in the production of the antibody-like factor adherent and non-adherent cells, and phagocytes.
Allison,
An examination of the cytotoxic effects of silica on macrophages.
1966, Pubmed
Allison,
An examination of the cytotoxic effects of silica on macrophages.
1966,
Pubmed
Brillouet,
Specific immune response in the sea star Asterias rubens: production of "antibody-like" factors.
1984,
Pubmed
,
Echinobase
Brillouet,
In vitro effect of various mitogens on starfish (Asterias rubens) axial organ cells.
1981,
Pubmed
,
Echinobase
Chen,
The effects of mercaptoethanol and of peritoneal macrophages on the antibody-forming capacity of nonadherent mouse spleen cells in vitro.
1972,
Pubmed
Delfraissy,
Primary in vitro antibody response of human peripheral blood lymphocytes: role of phagocytic mononuclear cells.
1978,
Pubmed
Julius,
A rapid method for the isolation of functional thymus-derived murine lymphocytes.
1973,
Pubmed
Kågedal,
Binding of covalent proteins to polysaccharides by cyanogen bromide and organic cyanates. I. Preparation of soluble glycine-, insulin- and ampicillin-dextran.
1971,
Pubmed
Leclerc,
The starfish axial organ: an ancestral lymphoid organ.
1980,
Pubmed
,
Echinobase
Leclerc,
Properties of cell subpopulations of starfish axial organ: in vitro effect of pokeweed mitogen and evidence of lymphokine-like substances.
1981,
Pubmed
,
Echinobase
Leclerc,
Correlation between the injection of specific proteins in starfishes and the appearance of binding sites for the same proteins in cell populations of their axial organ.
1980,
Pubmed
,
Echinobase
Lemke,
Function of 2-mercaptoethanol as a macrophage substitute in the primary immune response in vitro.
1976,
Pubmed
Mishell,
Immunization of dissociated spleen cell cultures from normal mice.
1967,
Pubmed
Möller,
Spleen cells from animals tolerant to a thymus-dependent antigen can be activated by lipopolysaccharide to synthesize antibodies against the tolerogen.
1976,
Pubmed
Rittenberg,
Antitrinitrophenyl (TNP) plaque assay. Primary response of Balb/c mice to soluble and particulate immunogen.
1969,
Pubmed
