Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Proc Natl Acad Sci U S A
1987 Dec 01;8424:8937-40. doi: 10.1073/pnas.84.24.8937.
Show Gene links
Show Anatomy links
Heat-labile regulatory factor is required for 3'' processing of histone precursor mRNAs.
Gick O
,
Krämer A
,
Vasserot A
,
Birnstiel ML
.
???displayArticle.abstract???
In addition to Sm antigen-type small nuclear ribonucleoprotein particle(s) [snRNP(s)], at least one more factor is involved in the in vitro 3'' processing of histone precursor mRNAs (pre-mRNAs) in a HeLa cell nuclear extract. This factor can be completely inactivated by mild heat treatment but is resistant to digestion by micrococcal nuclease and is not immunoprecipitated by antisera of the Sm serotype. Both snRNP (the presumed human homologue of the U7 snRNP of the sea urchin) and the heat-labile factor described above show closely similar properties when fractionated on DEAE, heparin, and Mono Q columns. Fractions, after extensive purification, still contain both heat-labile factor and snRNP activity. When analyzed by gel filtration, the heat-labile component distributes bimodally, the smaller component possessing an apparent molecular weight on the order of 40,000, and the larger, of ca. 300,000.
Berget,
U1, U2, and U4/U6 small nuclear ribonucleoproteins are required for in vitro splicing but not polyadenylation.
1986, Pubmed
Berget,
U1, U2, and U4/U6 small nuclear ribonucleoproteins are required for in vitro splicing but not polyadenylation.
1986,
Pubmed
Birnstiel,
Transcription termination and 3' processing: the end is in site!
1985,
Pubmed
Chabot,
The 3' splice site of pre-messenger RNA is recognized by a small nuclear ribonucleoprotein.
1985,
Pubmed
De Robertis,
Intracellular transport of microinjected 5S and small nuclear RNAs.
1982,
Pubmed
Galli,
Biochemical complementation with RNA in the Xenopus oocyte: a small RNA is required for the generation of 3' histone mRNA termini.
1983,
Pubmed
,
Echinobase
Gerke,
A protein associated with small nuclear ribonucleoprotein particles recognizes the 3' splice site of premessenger RNA.
1986,
Pubmed
Gick,
Generation of histone mRNA 3' ends by endonucleolytic cleavage of the pre-mRNA in a snRNP-dependent in vitro reaction.
1986,
Pubmed
,
Echinobase
Hashimoto,
A small nuclear ribonucleoprotein associates with the AAUAAA polyadenylation signal in vitro.
1986,
Pubmed
Krainer,
Multiple factors including the small nuclear ribonucleoproteins U1 and U2 are necessary for pre-mRNA splicing in vitro.
1985,
Pubmed
Krämer,
Purification of a protein required for the splicing of pre-mRNA and its separation from the lariat debranching enzyme.
1985,
Pubmed
Krämer,
Fractionation of HeLa cell nuclear extracts reveals minor small nuclear ribonucleoprotein particles.
1987,
Pubmed
Lerner,
Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus.
1979,
Pubmed
Lüscher,
RNA 3' processing regulates histone mRNA levels in a mammalian cell cycle mutant. A processing factor becomes limiting in G1-arrested cells.
1987,
Pubmed
Lüscher,
Faithful cell-cycle regulation of a recombinant mouse histone H4 gene is controlled by sequences in the 3'-terminal part of the gene.
1985,
Pubmed
Maniatis,
The role of small nuclear ribonucleoprotein particles in pre-mRNA splicing.
1987,
Pubmed
Moore,
Accurate cleavage and polyadenylation of exogenous RNA substrate.
1985,
Pubmed
Mowry,
Both conserved signals on mammalian histone pre-mRNAs associate with small nuclear ribonucleoproteins during 3' end formation in vitro.
1987,
Pubmed
Schaufele,
Compensatory mutations suggest that base-pairing with a small nuclear RNA is required to form the 3' end of H3 messenger RNA.
1986,
Pubmed
,
Echinobase
Sharp,
Splicing of messenger RNA precursors.
1987,
Pubmed
Stauber,
A signal regulating mouse histone H4 mRNA levels in a mammalian cell cycle mutant and sequences controlling RNA 3' processing are both contained within the same 80-bp fragment.
1986,
Pubmed
Strub,
Genetic complementation in the Xenopus oocyte: co-expression of sea urchin histone and U7 RNAs restores 3' processing of H3 pre-mRNA in the oocyte.
1986,
Pubmed
,
Echinobase
Strub,
The cDNA sequences of the sea urchin U7 small nuclear RNA suggest specific contacts between histone mRNA precursor and U7 RNA during RNA processing.
1984,
Pubmed
,
Echinobase
Tazi,
A protein that specifically recognizes the 3' splice site of mammalian pre-mRNA introns is associated with a small nuclear ribonucleoprotein.
1986,
Pubmed
